Self-Assembled Magnetic Matrices for DNA Separation Chips

Doyle, Patrick S.; Bibette, Jérôme; Bancaud, Aurélien; Viovy, Jean‐Louis

doi:10.1126/science.1068420

Cited by 456 publications

(313 citation statements)

References 6 publications

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“…This represents a convenient solution, since no microlithography is required to define geometrical constrictions that are simply defined by the porous magnetic matrix (Doyle et al 2002). Figure 11a is a schematic diagram of the microchannel and magnetic coil system.…”

Section: Separation Using Magnetic Supraparticle Structuresmentioning

confidence: 99%

Magnetic bead handling on-chip: new opportunities for analytical applications

Gijs

2004

Microfluid Nanofluid

497

555

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This review describes recent advances in the handling and manipulation of magnetic particles in microfluidic systems. Starting from the properties of magnetic nanoparticles and microparticles, their use in magnetic separation, immuno-assays, magnetic resonance imaging, drug delivery, and hyperthermia is discussed. We then focus on new developments in magnetic manipulation, separation, transport, and detection of magnetic microparticles and nanoparticles in microfluidic systems, pointing out the advantages and prospects of these concepts for future analysis applications.

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Section: Separation Using Magnetic Supraparticle Structuresmentioning

confidence: 99%

Magnetic bead handling on-chip: new opportunities for analytical applications

Gijs

2004

Microfluid Nanofluid

497

555

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“…As compared to our earlier work on self-assembly (21)(22)(23), the use of magnetic ink templates has two advantages: (i) First the magnetic potential wells impose onto the magnetic columns a perfectly predefined order, instead of the glass-like order obtained at long range with spontaneous self-assembly (21); (ii) Second, the magnetic wells stabilize the array, and allow much higher flow rates.…”

mentioning

confidence: 99%

“…The stability of this array decreases when the microchannel thickness and/or the column spacing increase. In practice, arrays with a spacing of 2-5 μm are easy to achieve with a good reproducibility, and were previously used in our laboratory for the separation of DNA (22,23). In contrast, arrays with column spacing larger than 10 μm are irregular and fragile.…”

mentioning

confidence: 99%

Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays

Saliba

Saias

Psychari

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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We propose a unique method for cell sorting, "Ephesia," using columns of biofunctionalized superparamagnetic beads selfassembled in a microfluidic channel onto an array of magnetic traps prepared by microcontact printing. It combines the advantages of microfluidic cell sorting, notably the application of a well controlled, flow-activated interaction between cells and beads, and those of immunomagnetic sorting, notably the use of batch-prepared, well characterized antibody-bearing beads. On cell lines mixtures, we demonstrated a capture yield better than 94%, and the possibility to cultivate in situ the captured cells. A second series of experiments involved clinical samples-blood, pleural effusion, and fine needle aspirates-issued from healthy donors and patients with B-cell hematological malignant tumors (leukemia and lymphoma). The immunophenotype and morphology of B-lymphocytes were analyzed directly in the microfluidic chamber, and compared with conventional flow cytometry and visual cytology data, in a blind test. Immunophenotyping results using Ephesia were fully consistent with those obtained by flow cytometry. We obtained in situ high resolution confocal three-dimensional images of the cell nuclei, showing intranuclear details consistent with conventional cytological staining. Ephesia thus provides a powerful approach to cell capture and typing allowing fully automated high resolution and quantitative immunophenotyping and morphological analysis. It requires at least 10 times smaller sample volume and cell numbers than cytometry, potentially increasing the range of indications and the success rate of microbiopsy-based diagnosis, and reducing analysis time and cost.lab-on-a-chip | magnetic beads | cell sorting | cancer diagnosis C ell-based screening is a major tool in medicine and pharmaceutical research. In oncology and haematology, the morphological and phenotypic typing of cancer cells is already used routinely for diagnostic and therapeutic purposes. This typing is made all the more relevant by the development of "personalized medicine" approaches, that of new anticancer drugs targeting specific mutations, such as trastuzumab or rituximab, which require specific tumor cell typing regarding HER2 and CD20 expression, respectively

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“…9 Recent advances in photolithography allow the production of magnetic gradients exceeding 10 5 T / m in microelectromechanical systems ͑MEMS͒ devices, 10 enabling the manipulation of isolated magnetic microspheres in a controlled fashion. Functionalized walls or magnetic microspheres can be used for protein microarrays; 11 DNA purification; 12 DNA sequencing and separation; 13 in vitro cell manipulation and separation; 14 and in vivo drug targeting. 15 The predominant transport issue here is the precise magnetic manipulation of microbeads in a microfluidic flow.…”

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confidence: 99%

Single magnetic particle dynamics in a microchannel

Sinha

Ganguly

et al. 2007

Physics of Fluids

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Functionalized magnetic particles are used in micrototal analysis systems since they act as magnetically steered mobile substrates in microfluidic channels, and can be collected for bioanalytical processing. Here, we examine the motion of magnetic microbeads in a microfluidic flow under the influence of a nonuniform external magnetic field and characterize their collection in terms of the magnetic field strength, particle size, magnetic susceptibility, host fluid velocity and viscosity, and the characteristic length scale. We show that the collection efficiency of a magnetic collector depends upon two dimensionless numbers that compare the magnetic and particle drag forces.

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Self-Assembled Magnetic Matrices for DNA Separation Chips

Cited by 456 publications

References 6 publications

Magnetic bead handling on-chip: new opportunities for analytical applications

Magnetic bead handling on-chip: new opportunities for analytical applications

Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays

Single magnetic particle dynamics in a microchannel

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