Usually a MOST of I/O cells in integrated circuits will be in the form of multi-finger type. However, the non-uniform turned on phenomenon in an MOST is deeply affecting the ESD reliability robustness. Here, the impacts of pick-up stripe variation and a pWell structure adding are investigated in this paper. ESD performance of these nMOSTs fabricated by a O.351lm CMOS process is evaluated in this work. Nevertheless, it is desirous to improve the ESD capability of ESD elements. After a systematic analysis, it is found that no matter what kind of channel length of nMOSTs, the p + pick-up structure of source side and p-well structure in the O.351lm LV process are poor contributors to 1 t2 robustness of elements, i.e., the substrate pick-up/ p-well structures will obviously lower the 1 t2 level. Therefore, the source end should avoid adding any p + pick-up stripe and any p-well structure in the O.351lm process.