Sensitive determination of reactive oxygen species in cigarette smoke using microchip electrophoresis–localized surface plasmon resonance enhanced fluorescence detection

Abstract

A sensitive approach to the determination of reactive oxygen species (ROS) in puffs of cigarette smoke (CS) has been developed. The experimental system consists of a microfluidic chip electrophoresis and a laser induced fluorescence (LIF) device enhanced by localized surface plasmon resonance. Core-shell Ag@SiO2 nanoparticles were prepared and then immobilized on the surface of the microchannel to increase the fluorescence intensity based on localized surface plasmon resonance-enhanced fluorescence (LSPREF) effect. The ROS in puffs of CS were trapped via the oxidation of 2',7'-dichlorodihydrofluorescein (DCHF) that had been loaded on polyacrylonitrile (PAN) nanofibers in a micro-column. Determination of ROS was based on the amount of 2',7'-dichlorofluorescein (DCF), which is the sole product from DCHF oxidation. With the optimization of the trapping efficiency, we detected about 8.0 pmol of ROS per puff in the mainstream CS. This microchip electrophoresis-SPREF system enables sensitive quantitation of ROS in CS with low consumption of reagent, material, and analysis time.

Fluorescent Sulfur-Tagged Europium(III) Coordination Polymers for Monitoring Reactive Oxygen Species

“…The gasphase trapping is completed using a device similar as described in our previous work ( Figure 4A). 25,30 The main stream CS is sampled using an improved puffing method, i.e., drawing 35 mL of smoke every 20 s within 16 s duration. 25 After trapping the ROS in main stream CS, the fluorescence emission of CP-25 superstructures is then detected with an inverted fluorescence microscope.…”

“…25,30 The main stream CS is sampled using an improved puffing method, i.e., drawing 35 mL of smoke every 20 s within 16 s duration. 25 After trapping the ROS in main stream CS, the fluorescence emission of CP-25 superstructures is then detected with an inverted fluorescence microscope. As shown in Figure 4B, the fluorescent images of CP-25 superstructures are continuously quenched with the increase of numbers of puffs.…”

“…The prepared SPME fiber was fabricated in a smoke-trapping device. As shown in Figure 4A, during the ROS trapping progress, the mainstream CS was passed through a microcolumn, which was loaded with 3 mg of polyacrylonitrile (PAN) nanofibers 25 for blocking the particulate matters. When the mainstream CS flowed out the microcolumn, the CP-25 superstructures, positioned at the end of the microcolumn, can react with the ROS in due time.…”

Utilisation of micro- and nanoscaled materials in microfluidic analytical devices

“…The system was used to analyse reactive oxygen species (ROS) in puffs of cigarette smoke. Determination of ROS was based on the amount of 2′, 7′-dichlorofluorescein that had been loaded on polyacrylonitrile nanofibers in a micro-column and which trapped the ROS [101].…”

Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection

“…[2][3][4][5][6] Highly enhanced local electromagnetic fields created by the resonantly pumped plasmonic nanostructures result in greatly increased incident light absorption and enhanced emitter excitation, supporting surface-enhanced spectroscopies including surface-enhanced Raman scattering, 7,8 surface-enhanced infrared spectroscopy, [9][10][11] and surface plasmon-enhanced fluorescence (SPFS). [12][13][14] Surface plasmons (SPs) with field E SP excited by an incoming light E 0 can enhance fluorophore excitation rate and Raman scattering by factors up to |E sp /E 0 | 2 and |E sp /E 0 | 4 , respectively. [15][16][17][18] Various metal nanostructures have been reported for the enhancement of fluorescence emission, such as nanoholes, [19][20][21] nanorods, 22,23 core-shell nanoparticles, [24][25][26] bowtie nanoantennas, 27,28 DNA-assembled nanoparticles, 29 and antennas-in-box.…”