Senyawa Steroid Spons Xestospongia sp. dari Perairan Sulawesi Tenggara

Sadarun, Baru; Malaka, Muhammad Hajrul; Wahyuni, Wiwik; Sahidin, S

doi:10.33772/pharmauho.v4i1.4628

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…These bonds activate the NF-κB as cytokines transcription factors thus increase the secretion of IFN-γ levels to extra cells. In addition, it also increases the T-cell stimulation activity as a response in pathogenesis (Egbuna, 2019;Delves, 2017;Sadarun, 2018). IFN-γ is the main MAC cytokine (macrophage activation cytokine) that activates macrophages thus phagocytosis progresses rapidly and increases the differentiation of CD4+ cells into Th1 cell subset.…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory Activity of Xestospongia sp. Ethanolic Extract Towards Interferon-gamma (IFN- γ) and Tumor Necrosis Factor-alpha (TNF-α) Levels in Wistar Male Rats

Fristiohady¹,

Jumadil

Wahyuni

et al. 2020

JFG

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Xestospongia sp. is one of marine sponge belongs to demosponges class that mainly found in Southeast Sulawesi and the secondary metabolites contained in Xestospongia sp. are suspected of having immunomodulatory activity. A previous study exhibited the immunomodulatory of Xestospongia sp. ethanolic extract (XEE) at dose of 300 and 400 mg/KgBW by affecting the phagocytic activity of macrophages. Thus, this study aims to investigate the effect of XEE towards interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) at dose of 300 and 400 mg/KgBW. Wistar male rats are divided into 4 groups (n=6) randomly and treated for 7 days orally each as follow: group I (XEE dose of 300 mg/KgBW); group II (XEE dose of 400 mg/KgBW); group III (0.5% NaCMC); and group IV (Phylantii extract commercial). On day 8, animals were infected with Staphylococcus aureus and left for 1 hour. Animals were sacrificed and the blood was drawn by cardiac puncture (3 mL), followed by analyzed under ELISA Kit for IFN-γ and TNF-α. Collected data were analyzed statistically using SPSS®. The IFN-γ levels obtained were 350.113; 392.970; 118.416; and 61.958 ρg/mL, respectively and the TNF-α were 2808; 1308; 778; and 845.5 ρg/mL, respectively. According to results obtained, both doses of XEE are affecting the IFN-γ and TNF-α levels (p<0.05) compared to group III as negative control, and group IV as positive control. Concluding that XEE of both doses is increasing IFN-γ and TNF-α levels of animals that respond to phagocytic activity.

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Section: Resultsmentioning

confidence: 99%

Immunomodulatory Activity of Xestospongia sp. Ethanolic Extract Towards Interferon-gamma (IFN- γ) and Tumor Necrosis Factor-alpha (TNF-α) Levels in Wistar Male Rats

Fristiohady¹,

Jumadil

Wahyuni

et al. 2020

JFG

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“…Sebarannya mulai dari zona intertidal hingga zona subtidal pada suatu perairan (Haedar et al, 2016). Spons diketahui memiliki banyak senyawa aktif yang mempunyai potensi sebagai obat-obatan (Sadarun et al, 2017(Sadarun et al, , 2018Sahidin et al, 2018) Genus Petrosia merupakan salah satu jenis spons yang tingkat kemunculannya tinggi khususnya jenis di perairan Sulawesi Tenggara (Rahman et al, 2020). Potensi pengembangan Petrosia sp.…”

Section: Pendahuluanunclassified

Isolation and Identification of Secondary Metabolite From Ethyl Acetate Extract of Petrosia Sp. And Its Antioxidant Activity

Malaka

Hartina

Fristiohady

et al. 2022

JFSP

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Sponges are marine organism that contain various chemical compound with potential pharmacological activities. This study aims to isolate and identify secondary metabolites from ethyl acetate extract of Petrosia sp. and its antioxidant properties. Extraction was performed by maceration using ethyl acetate. Isolation methods were carried out using Thin Layer Chromatography (TLC), Vacuum Liquid Chromatography (VLC), and Radial Chromatography (RC) technique. Isolated compound was identified by 1D-NMR (1H and 13C NMR) and 2D-NMR (HMQC and HMBC). Antioxidant assay was determined by its activity against ABTS radical. Isolated compound identified as an alkaloid namely 6-hydroxy-1,3,7-trimethyl-3,5-dihydro-1-Himidazo [4,5-c] piridine -2,4-dione. Antioxidant activity test showed Petrosia sp. extract was active as antioxidant with IC50 values 27.20 μg/mL against DPPH and 27.53 μg/mL against ABTS, whereas isolated compound was inactive against DPPH and ABTS with IC50 values 242.64 μg/mL. Meanwhile, Vitamin C was very active against DPPH and ABTS with IC50 21.51 μg/mL and 22.21 μg/mL, respectively.

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Anti-inflammatory Activity of Pharmaceutical Gel of Ethanolic Extract from Marine Sponge Xestospongia Sp

et al. 2019

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The aim of this study was to determine the characteristics of the gel formula based on it�s organoleptic properties, pH, viscosity, dispersion and homogenity by stability test carried out using the cycling test method and to determine the anti-inflammatory activity of the Xestospongia Sp. in male white mice (Mus musculus) by creating an artificial edema on the mice left foot induced by 1% ?-carrageenan. The gel formula from the ethanol extract of Xestospongia Sp. sponge was physically stable in terms of its organoleptic observation, homogenity, pH and viscosity test. However, the results that were obtained after dispersion test did not fulfill the requirements. In this study, the gel formula of the ethanol extract of Xestospongia Sp. sponge was administered on the mice left foot by using the variations in extract concentration of 0.02%, 0.03%, and 0.04%, and the gel without extract as a negative control and Galtaren�gel (1% Diclofenac Sodium) as a positive control. The evaluated data were in the form of mice leg edema volume measured based on its percent of inflammation and percent of inflammatory inhibition and observed for 360 minutes. The data were analyzed by using the Kruskal-Wallis test followed by the Mann-Whitney test with a confidence level of 95%. The results of this study showed that the gel formula of ethanol extract of Xestospongia Sp. sponge has an anti-inflammatory effect on each concentration and the formula that has a large anti-inflammatory effect was obtained at extract concentration of 0.04%.

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Senyawa Steroid Spons Xestospongia sp. dari Perairan Sulawesi Tenggara

Cited by 3 publications

References 6 publications

Immunomodulatory Activity of Xestospongia sp. Ethanolic Extract Towards Interferon-gamma (IFN- γ) and Tumor Necrosis Factor-alpha (TNF-α) Levels in Wistar Male Rats

Immunomodulatory Activity of Xestospongia sp. Ethanolic Extract Towards Interferon-gamma (IFN- γ) and Tumor Necrosis Factor-alpha (TNF-α) Levels in Wistar Male Rats

Isolation and Identification of Secondary Metabolite From Ethyl Acetate Extract of Petrosia Sp. And Its Antioxidant Activity

Anti-inflammatory Activity of Pharmaceutical Gel of Ethanolic Extract from Marine Sponge Xestospongia Sp

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