Separation and simultaneous determination of seven bioactive components in <i>Tripterygium wilfordii</i> Hook. F. and <i>Tripterygium</i> preparations by micellar electrokinetic capillary chromatography

Guo, Haitao; Wang, Zhiying; Xu, Lu; Zhang, Hongfen; Chang, Ruimiao; Chen, Anjia

doi:10.1002/elps.201800455

Cited by 5 publications

(4 citation statements)

References 26 publications

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“…Further, the complex structure of the pentacyclic triterpenoid celastrol highlights the complexity of plant extracts. After induction of CMCs of T. wilfordii in our work, UPLC results showed that the production of triptolide, celastrol and triptophenolide in CMCs was 53, 10 and 4.62 times higher than those of the T. wilfordii plant which has been reported previously, respectively [38, 39].…”

Section: Discussionsupporting

confidence: 81%

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A novel strategy to enhance terpenoids production using cambial meristematic cells of Tripterygium wilfordii Hook. f.

et al. 2019

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Background Tripterygium wilfordii Hook. f. (T. wilfordii) is an important medicinal plant with anti-inflammatory, immunosuppressive and anti-tumor activities. The main bioactive ingredients are diterpenoids and triterpenoids, such as triptolide, triptophenolide and celastrol. However, the production of terpenoids from original plants, hairy roots and dedifferentiated cells (DDCs) are not satisfactory for clinical applications. To find a new way to further improve the production of terpenoids, we established a new culture system of cambial meristematic cells (CMCs) with stem cell-like properties, which had strong vigor and high efficiency to produce large amounts of terpenoids of T. wilfordii. Results CMCs of T. wilfordii were isolated and cultured for the first time. CMCs were characterized consistent with stem cell identities based on their physiological and molecular analysis, including morphology of CMCs, hypersensitivity to zeocin, thin cell wall and orthogonal partial least square-discriminant analysis, combination of transcriptional data analysis. After induction with methyl jasmonate (MJ), the maximal production of triptolide, celastrol and triptophenolide in CMCs was 312%, 400% and 327% higher than that of control group, respectively. As for medium, MJ-induced CMCs secreted 231% triptolide and 130% triptophenolide at the maximum level into medium higher than that of control group. Maximal celastrol production of induced CMCs medium was 48% lower than that of control group. Long-term induction significantly enhanced the production of terpenoids both in cells and medium. The reason for increasing the yield of terpenoids was that expression levels of 1-deoxy-d-xylulose-5-phosphate synthase (DXS), 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR) and hydroxymethylglutaryl-CoA synthase (HMGS) were upregulated in CMCs after induction. Conclusions For the first time, CMCs of T. wilfordii were isolated, cultured, characterized and applied. Considering the significant enrichment of terpenoids in CMCs of T. wilfordii, CMCs could provide an efficient and controllable platform for sustainable production of terpenoids, which can be a better choice than DDCs.

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Section: Discussionsupporting

confidence: 81%

“…Most bioactive terpenoids are present in low amounts in the T. wilfordii plant with the mean concentrations of 7.61, 53.83 and 179 μg g −1 for triptolide, celastrol and triptophenolide, respectively [38, 39]. Further, the complex structure of the pentacyclic triterpenoid celastrol highlights the complexity of plant extracts.…”

Section: Discussionmentioning

confidence: 99%

A novel strategy to enhance terpenoids production using cambial meristematic cells of Tripterygium wilfordii Hook. f.

et al. 2019

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“…In MEKC, the surfactants are used as additives in the running buffer and the concentration higher than the critical micelle concentration (CMC) to provide a micellar pseudo‐stationary phase. The analytes are distributed between the aqueous phase and the micellar phase and migrated with the EOF in the capillary to achieve separation . Two kinds of surfactant, SDS and SC were investigated.…”

Section: Resultsmentioning

confidence: 99%

“…The analytes are distributed between the aqueous phase and the micellar phase and migrated with the EOF in the capillary to achieve separation. 23,24 Two kinds of surfactant, SDS and SC were investigated. SDS was ineffective for the separation of hirsutine and hirsuteine.…”

Section: Concentration Of Scmentioning

confidence: 99%

Simultaneous separation and determination of hirsutine and hirsuteine by cyclodextrin‐modified micellar electrokinetic capillary chromatography

Guo

Wang

Zhang

et al. 2019

Phytochemical Analysis

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Introduction Hirsutine and hirsuteine are the main pharmacological activity ingredients of Uncaria rhynchophylla (UR), playing an important role in treating mental and cardiovascular diseases, such as Alzheimerʼs disease, hypertension, Parkinson's disease, potential anti‐cancer activities and so on. Objective To develop a cyclodextrin‐modified micellar electrokinetic capillary chromatography (CD‐MEKC) method for the simultaneous separation and determination of hirsutine and hirsuteine from UR and its formulations. Methodology The optimal method was developed by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of hirsutine and hirsuteine in UR and its formulations. Results The optimal background electrolyte (BGE) consisted of 40 mM sodium dihydrogen phosphate (pH 7.0), 150 mM 2,6‐dimethyl‐β‐cyclodextrin (DM‐β‐CD), 3 mM mono‐(6‐ethylenediamine‐6‐deoxy)‐β‐cyclodextrin (ED‐β‐CD), and 30 mM sodium cholate (SC). Under these conditions, hirsutine and hirsuteine were successfully separated within 13 min at the separation voltage of 15 kV, temperature of 25°C and the detection wavelength of 224 nm. For the analytes, linear calibration curves were performed within the range 5.0–160.0 μg/mL. The limit of detection (LOD, S/N = 3) and the limit of quantitation (LOQ, S/N = 10) were 0.41, 1.42 μg/mL for hirsutine and 0.60, 2.17 μg/mL for hirsuteine, respectively. The recoveries of three samples were from 97.9% to 102.3%. Conclusion The method was successfully applied to the determination of hirsutine and hirsuteine in UR and its formulations. Meanwhile, it provides an effective reference of the quality control of UR and its formulations.

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Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography

Wang

Yang

Guo

et al. 2021

Phytochemical Analysis

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Introduction: Picfeltarraenins IA, IB and IV and acteoside are the four bioactive ingredients of Picria fel-terrae Lour. Their pharmacological effects include central inhibitory, cardiovascular, anti-inflammatory, anti-pyretic, analgesic, anti-bacterial, antioxidative and anti-tumor effects.Objective: We aimed to develop an efficient micellar electrokinetic chromatography (MEKC) method modified with mixed organic solvents for the simultaneous separation and determination of the four components in Picriae Herba and its formulations.Methods: Method optimization was carried out by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of the four components in Picriae Herba and its formulations.Results: The optimal running buffer was composed of 20 mM sodium tetraborate, 40 mM sodium cholate, 10% (v/v) methanol and 10% (v/v) isopropanol (pH 9.76).The separation voltage was 18 kV, the temperature was 25 C and the detection wavelength was 266 nm. Under the optimal separation conditions, the baseline separation of four components was achieved in less than 14 min. The correlation coefficients of the calibration curves were 0.9984-0.9995 for the analytes. The intraday and interday precision ranged from 1.5% to 2.5% and from 1.4% to 5.0%, respectively. Recoveries of analytes varied from 96.6% to 104.1%. Conclusion:The method was proved suitable for the determination of four components in Picriae Herba and its formulations. Good performance was obtained under optimal conditions, and the method provides an effective tool for the quality control of Picriae Herba and its formulations.

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Separation and simultaneous determination of seven bioactive components in Tripterygium wilfordii Hook. F. and Tripterygium preparations by micellar electrokinetic capillary chromatography

Cited by 5 publications

References 26 publications

A novel strategy to enhance terpenoids production using cambial meristematic cells of Tripterygium wilfordii Hook. f.

A novel strategy to enhance terpenoids production using cambial meristematic cells of Tripterygium wilfordii Hook. f.

Simultaneous separation and determination of hirsutine and hirsuteine by cyclodextrin‐modified micellar electrokinetic capillary chromatography

Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography

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