Serial clinical, colpo-cytological and endocrinological evaluations of Cerdocyon thous bitches from the Rio de Janeiro zoo

Monteiro, Rafael Veríssimo; Santos, Márcio Ricardo Costa dos; Vianna, Eduardo Borges; Araújo, Tatiana Cardoso Soares de; Verona, Carlos Eduardo; Fedullo, Luís Paulo Luzes

doi:10.1590/s1413-95962002000200007

Cited by 7 publications

(8 citation statements)

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“…It requires few supplies, and, with some experience, the assessments can be easily performed in fresh, unstained smears, or in fixed, stained ones (Goldman et al, ). This technique has been used to establish sexual receptivity and ensure better reproductive performance and preservation of genetic variability in studies using rodents, such as C. porcellus (Lilley et al, ; Selle, ), Dasyprocta prymnolopha (Guimarães et al, ), Cavia aperea and Galea musteloides (Touma et al, ), Agouti paca (Guimarães et al, ), Myocastor coypus (Felipe et al, ) and other species of wild mammals as Cerdocyon thus (Monteiro et al, ), Bradypus torquatus (Snoeck et al, ) and domestic mammals (Allison et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Myocastor coypus (Felipe et al, 2001) and other species of wild mammals as Cerdocyon thus (Monteiro et al, 2002), Bradypus torquatus (Snoeck et al, 2011) and domestic mammals (Allison et al, 2008).…”

Section: At Proestrus Large Intermediate Cells and Nucleated And Enumentioning

confidence: 99%

“…Then, vaginal cytology has been used to determine estrous cycle phases in small rodents used as experimental model (Bastos et al, ; Goldman et al, ; Guimarães et al, ; Lilley et al, ; Mahoney et al, ; Marcondes et al, ; Touma et al, ). Other researchers also have used this tool to detect characteristics related to the receptivity of the female for copulation in order to assist captive breeding programs in endangered wild animals (Felipe et al, ; Guimarães et al, ; Monteiro et al, ) and embryo transfer and artificial insemination techniques in livestock animals (Binelli et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Other researchers also have used this tool to detect characteristics related to the receptivity of the female for copulation in order to assist captive breeding programs in endangered wild animals (Felipe et al, 1999;Guimarães et al, 2011;Monteiro et al, 2002) and embryo transfer and artificial insemination techniques in livestock animals (Binelli et al, 2014).…”

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confidence: 99%

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Follicular development and morphological changes in the vaginal epithelium during the estrous cycle ofGalea spixii

Santos¹,

Viana²,

Oliveira

et al. 2016

Microsc. Res. Tech.

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The current study aimed to determine if characteristics observed in vaginal cytology during the estrous cycle of female SYT cavies corresponded with proliferation of the vaginal epithelium, characterized by proliferating cell nuclear antigen (PCNA) immunolocalization, and with follicular development at different phases of the estrous cycle. After determining estrous cycle phases by vaginal cytology, females were euthanized at metestrus, diestrus, proestrus, and estrus. Histological study of the vaginal epithelium and ovary were then performed. Immunohistochemistry for PCNA in vaginal tissue at each cycle phase was also performed. Superficial cornified cells and early post-ovulatory follicles were found at estrus. Few nuclei below the enucleate superficial cells were immunoreactive to PCNA. At metestrus, the vaginal epithelium underwent desquamation and lost the superficial cornified cells; basal and intermediate cells appeared, and the post-ovulatory follicle formed an early corpus luteum. No PCNA immunoreactivity was observed. At diestrus, the corpus luteum was developed, and the vaginal epithelium contained basal and intermediate cells. There was PCNA immunoreactivity in the cellular nucleus in the germinative stratum of the epithelium. Because of the growth and maturation of ovarian follicles, the vaginal epithelium suffered intense proliferation at proestrus. Vaginal cytology revealed large intermediate cells and nucleated and enucleated superficial cornified cells. In the ovary, mature follicles were present. More apparent immunoreactivity of PCNA in the germinative layer was found. In summary, we inferred that vaginal exfoliative findings matched the proliferation process of the vaginal epithelium. PCNA immunolocalization occurred as well as corresponding follicular development in the ovaries.

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Section: Discussionmentioning

confidence: 99%

Section: At Proestrus Large Intermediate Cells and Nucleated And Enumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Follicular development and morphological changes in the vaginal epithelium during the estrous cycle ofGalea spixii

Santos¹,

Viana²,

Oliveira

et al. 2016

Microsc. Res. Tech.

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“…Esta técnica, fácil e de baixo custo, consiste na pesquisa de diferentes células do epitélio vaginal e é usada normalmente para determinar as fases do ciclo estral em cadelas e gatas (Johnston et al 2001), a fim de predizer o melhor momento para realização da có-pula ou inseminação artificial. Algumas pesquisas têm sido realizadas com ursos panda (Durrant et al 2002, Durrant et al 2006, leões africanos (Silva et al 2006), caititus (Lima Neto & Nogueira Filho 2002), tamanduás (Soboll 2008) e cachorro do mato (Monteiro et al 2002). Todas buscaram observar se houve a existência de um mesmo padrão citológico encontrado em cães e gatos e identificar o melhor momento para realizar reprodução assistida ex situ nessas espécies.…”

Section: Introductionunclassified

Citologia vaginal de preguiça-de-coleira (Bradypus torquatus)

Snoeck

Cruz²,

Catenacci³

et al. 2011

Pesq. Vet. Bras.

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RESUMO.-As preguiças-de-coleira (Bradypus torquatus) são mamíferos arborícolas da família Bradypodidae. Podem ser encontradas nos trechos de Mata Atlântica do Brasil e a maior diversidade genética de suas populações ocorre em matas do sul da Bahia. A observação desses animais na natureza é muito difícil, pois passam a maior parte da vida escondidos no denso emaranhado das copas, por isso, dados sobre aspectos reprodutivos são escassos e não existem informações sobre ciclo estral dessa espécie. Este trabalho teve por objetivo identificar as células do epitélio vaginal da preguiça-de-coleira (Bradypus torquatus) como forma de viabilizar o uso dessa técnica para estudar as fases do ciclo estral desses animais. As amostras para citologia Maned sloths (Bradypus torquatus) are arboreal mammals of the family Bradypodidae. They can be only found in the Atlantic coast forest of Brazil and its most genetically diverse populations occur in forests of southern Bahia. The observation of these animals in the wild is very difficult as they spend most of their lifetime hidden in the dense forest canopy. Data on their reproductive aspects are scarce, and there is none information about their estrous cycle. This research aimed at identifying the vaginal epithelial cells of maned sloths (Bradypus torquatus) as a possible way to study the phases of the estrous cycle of this animal. The samples for vaginal cytology were obtained from four free ranging maned sloths living in a protected area of coastal forest in the South of Bahia. The sterile gynecological brush was inserted up to the necessary distance to reach the pelvic channel. For each sample two smears were made by rotating the tip of the brush onto each glass slide, producing in general three linear impressions. Staining was performed using rapid Panotic Kit (Laborclin®). Maned sloths BT033, BT065, and BT042 presented, respectively, 30%, 33%, and 7% of parabasal epithelial cells (PB); 56%, 22%, and 10% of small intermediate cells (IP); 6%, 18%, and 6% of large intermediate cells (IG); 2%, 13%, and 24% of superficial epithelial cell with a nucleus (SN); 6%, 14%, and 53% of anucleated superficial epithelial cell (AS). Two cell samples were collected for maned sloth BT464 with a 13 months interval. Cytological differences were observed between the two samples (1st and 2nd): 6% and 17,5% of PB cells, 5% and 25% of IP cells, 11% and 15,5% of IG cells, 8% and 19,5% of SN cells and 70% and 22,5% of AS cells, respectively. It's interesting to remark that the percentage of vaginal epithelial cells varied among sloths and also for the same animal. This result suggests that vaginal cytology of maned sloth can be used as a tool to evaluate of estrous cycle.INDEX TERMS: Vaginal epithelial cells, reproduction, maned sloth, Bradypus torquatus, Bradypodidae.

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