Serological and Molecular Comparison Study for Diagnosis of Cytomegalovirus Infection in aborted Pregnant Women in Iraq

Al-Taie, Anmar; Abdullah, B. A.; Al-Attar, M. Y.

doi:10.33899/rjs.2018.159336

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…To ensure that all CMV IgM -positive samples for IgG avidity has been identified and screened. The present study found in Table 7 that the age group of 20 to 30 years recorded the highest incidence of microbial cytomegalovirus infections could increase the risk of cases of abortion in women, furthermore it is the less efficient as people grow older, and this finding was supported by our research agreement with the findings of the previous study (13) who reported that the majority of HCMV infections occurred in the 20-29 years age group. The occurrence of primary infections of CMV may be due to that most marriages in our population occurred among this age group.…”

Section: Serological Identification Of Igg and Igm Of CMVsupporting

confidence: 92%

“…Highly significant differences were detected between the studied groups regarding age (years) distribution as shown in Table5, eight of women were 17-20 years old, 24 were 20-24 years old, 24 were 25-29 years old, 21 were 30-39 and 13 were above 40 years old. The present study agrees with the study by Anmar et al(13) who found the highest seropositivity (29%) is shown in the age groups of (20-29) years.…”

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confidence: 94%

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Immunological and Molecular Diagnosis of Cytomegalovirus Infection between Aborted & Pregnant Women in Babylon City

Al-Mousawi

AL-Hajjar²

2021

Baghdad Sci.J

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Human cytomegalovirus (CMV) is the globally highly prevalent herpesvirus worldwide. CMV infects populations of all ages according to the Center for Disease Control and Prevention (CDC) and World Health Organization (WHO). CMV infections remain the most common viral complication potentially multiple in humans and are a major cause of congenital normality in women, which is why they are critical for diagnosis in several times when it happens during pregnancy. Pregnant women with CMV infection can be in charge of abortion or congenital expandaedby. This study involves the collection a total of (90) samples taken from each aborted and pregnant woman (70 with abortion cases and 20 of pregnant without history of abortion as control subjects) referring to Babylon teaching hospital for Maternity and Children, covering a period from (October 2018 to March 2019) to investigate the occurrence of Cytomegalovirus (CMV) in Babylon city. Patients and controls were evaluated for IgG, IgM antibodies and anti-HCMV IgG, IgM for (90) subjects were controlled in this study using the Enzyme Immunoassay Test Kit and read by enzyme - linked immunosorbent analyze (ELISA). In addition the polymerase chain reaction (PCR) DNA detection for CMV are based on the amplification of pathogen genomes in a particular region using different primers. The Chi-square test was used to analyze the data. The results show among 90 samples, women were evaluated for CMV infection, the seroprevalence titer was significantly higher at P <0.05 in seropositive cases ranging from 62(89%) toward positive CMV IgG, while the 65 (93%) of patients were positive CMV IgM from (70) women with abortions. By contrast, the results obtained from the controls were 9 (45%) subjects seropositive for IgG and all of them were seronegative with IgM. The anti – HCMV IgG finding showed high positivity that represents the furthermost of CMV infections among females through ages ranging between 20-29 years. Furthermore, the outcomes of molecular detection showed that a small number of samples 13 (19%) were HCMV DNA detectable in aborted women less than in pregnant women 3 (15%).

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Section: Serological Identification Of Igg and Igm Of CMVsupporting

confidence: 92%

supporting

confidence: 94%

Immunological and Molecular Diagnosis of Cytomegalovirus Infection between Aborted & Pregnant Women in Babylon City

Al-Mousawi

AL-Hajjar²

2021

Baghdad Sci.J

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“…Advantages of ELISA comprising of simplicity, cheapness and ability of determining the history of infection (early or late) make this method suitable for evaluating the presence of virus [42]. However, the less sensitivity and higher false results convinced some researchers to use other methods such as IHC or ISH to assess the presence of the virus [45]. Of the 29 studies reviewed, 13 studies used IHC or ISH techniques (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Human Cytomegalovirus in Breast Cancer: A Systematic Review

Nakhaie

Charostad

Ghaderi-Zefrehi

et al. 2021

Asian Pac J Cancer Biol

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Background: Breast cancer is the most common malignancy among women around the world, with several risk factors contributing to its progression. The role of infectious agents such as viruses in the progression of this cancer is relatively well-known, as a result of various studies in different geographical regions that reported the prevalence of cytomegalovirus in breast cancer. Objective: In this systematic review, we tried to examine the prevalence of cytomegalovirus in breast cancer. Materials and Methods: We conducted a comprehensive search of three databases: PubMed, Scopus, and Google Scolar up to January 21, 2021. Results: Out of 584 articles, 29 articles including 3158 samples were finally reviewed, of which 16 articles used PCR methods, 8 articles used serology method and 13 articles used other methods such as immunohistochemistry and in situ hybridization. The prevalence of the virus was higher in Asian and African countries such as Iraq and Egypt than other European and South American countries. Among these, the highest prevalence of the virus in different samples of patients with breast cancer was related to Iraq. Conclusion: The results of our study showed the higher prevalence of the virus in patients with breast cancer rather than normal subjects and reveal the role of the virus in the development of breast cancer.

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Association of Autoimmunity and Parvovirus B19 Infection among Spontaneous Miscarriage Women in Erbil / Iraq

Mohammed¹

2020

RJS

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The study was conducted from January 2016 to January 2017 at Maternity Teaching Hospital in Erbil city/ Iraq. One hundred and forty women who experienced spontaneous abortions were selected according to inclusion and exclusion criteria and forty normal pregnant women as a control group. Serum from patients and control group subjected for assessment of anticardiolipin IgM, antiphospholipid IgM, Parvovirus B19 IgG and IgM using enzyme linked immune-sorbent assay technique. Prevalence of positive Parvovirus B19 IgG and IgM, anticardiolipin IgM and antiphospholipid IgM, in aborted women was higher than that observed in non-aborted women (control group). Women with the highest frequency of spontaneous abortion found at age group 27-34 year. More frequent of previous spontaneous abortion was 1-2 abortion at the 1 st trimester of gestational period. Anticardiolipin antibody and antiphospholipid antibody production trigged by viral infection, and have role in spontaneous abortion in pregnant women.

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Serological and Molecular Comparison Study for Diagnosis of Cytomegalovirus Infection in aborted Pregnant Women in Iraq

Cited by 3 publications

References 19 publications

Immunological and Molecular Diagnosis of Cytomegalovirus Infection between Aborted & Pregnant Women in Babylon City

Immunological and Molecular Diagnosis of Cytomegalovirus Infection between Aborted & Pregnant Women in Babylon City

Prevalence of Human Cytomegalovirus in Breast Cancer: A Systematic Review

Association of Autoimmunity and Parvovirus B19 Infection among Spontaneous Miscarriage Women in Erbil / Iraq

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