Serological survey of caprine arthritis-encephalitis virus in 83 goat herds of Yucatan, Mexico

Torres-Acosta, J.F.J.; Gutiérrez-Ruiz, E. J.; Butler, von; Schmidt, Ana Carolina; Evans, Jenny; Babington, J.; Bearman, K.; Fordham, T.; Brownlie, Tom; Schroer, S.; Cámara-G, E.; Lightsey, J.

doi:10.1016/s0921-4488(03)00093-2

Cited by 25 publications

(17 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The export of European breeds of goats and sheep has resulted in the spread of SRLVs to various parts of the world [25,140]. In contrast, certain indigenous goat breeds that have had no contact with imported goat breeds, such as Toggenburg and Saanen in Europe or Criollo in Mexico [140], are free of CAE. Phylogenetic evidence for the introduction of SRLVs via live animal trade is provided by the similarity of the South African sheep lentivirus with European strains such as the Scottish EV1.…”

Section: Transmission Between Different Regionsmentioning

confidence: 99%

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

et al. 2004

View full text Add to dashboard Cite

-Small ruminant lentiviruses (SRLV = maedi-visna in sheep and caprine arthritis encephalitis in goats) are distributed throughout most countries of the world, particularly Europe. Laboratories from 16 European countries established collaborations within the framework of a COST (CO-operation in the field of Scientific and Technical Research) action sponsored by the European Union in order to (i) better organize their research programmes on SRLVs and (ii) to coordinate efforts to combat these two diseases. After five years, a consensus conference -the first one in the veterinary medicine field -concluded the work of this network of laboratories by reviewing the present position and discussing three important questions in the field of SRLVs: routes of transmission, consequences of infection and potential role of eradication programmes at either a European or local level, according to the situation in each country or region. This paper brings together existing information regarding these questions and identifies areas for future research.Maedi-visna / caprine arthritis-encephalitis virus / lentivirus / small ruminants / European consensus conference

show abstract

Section: Transmission Between Different Regionsmentioning

confidence: 99%

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Detection of the infection generally is performed by means of serological methods, such as indirect and blocking enzymelinked immunosorbent assays (ELISAs), agar gel immunodiffusion testing (AGIDT), and immunoblotting (3,7,9,10,18,20,21,22).…”

mentioning

confidence: 99%

Evaluation of Five Enzyme-Linked Immunosorbent Assays and an Agar Gel Immunodiffusion Test for Detection of Antibodies to Small Ruminant Lentiviruses

Brinkhof

Maanen

2007

Clin Vaccine Immunol

View full text Add to dashboard Cite

In the framework of the Dutch control program for small ruminant lentiviral (SRLV) infections, too many drawbacks were encountered with respect to serological testing. To improve the quality of testing, five enzyme-linked immunosorbent assays (ELISAs) and an agar gel immunodiffusion test (AGIDT) were evaluated. The focus was on the sensitivity, specificity, and variances of the commercially available tests. Clear differences were found among the tests in analytical and diagnostic sensitivity and overall diagnostic performance, whereas no significant differences in specificity were found.

show abstract

“…Our results showed that the prevalence of CAEV in goats in Iran was 15.7% which was higher than Mexico (3.6%) [22], Italy (4.0%) [23] and Great Britain (10.3%) [24] but entirely lower than USA (73%) [25] and Norway (86%) [26]. Serological study in Hungary showed that 30% of the Hungarian goat population was infected with CAEV [27].…”

Section: Discussionmentioning

confidence: 85%

The First Molecular Detection of Caprine Arthritis Encephalitis Virus (Caev) in Iran

Emami¹

2014

J Veterinar Sci Technol

View full text Add to dashboard Cite

Caprine arthritis encephalitis virus (CAEV) is a retrovirus that naturally infects goats and induces chronic inflammatory and degenerative disease. Infection of goats has a worldwide distribution but it has not been previously recognized by molecular method in Iran. For this reason, 95 blood samples were taken from 4 months to 3 years goats to determine naturally infected animals with caprine arthritis-encephalitis virus (CAEV) by using polymerase chain reaction (PCR) method. The PCR primers were designed to amplify a 433 base pair region of the proviral gag gene. Results showed that 15.7 percent of sampled goats (15 cases) were positive for CAEV. The present study was confirmed the presence of CAEV genome in goats flock of Iran for the first time. samples were taken from jugular vein and mononuclear cells (PBMC) were isolated from 10 ml EDTA-blood on a Ficoll-Hypaque gradient. They were then destroyed in buffer (100 mM Tris-HCl pH 7.5; 12.5mM EDTA-Na 2 ; 150 mM NaCl; 0.5% SDS) containing Proteinase K (Sigma) (50 µg per 10 7 PBMC) at 55° for 2 h. Genomic DNA was subsequently extracted twice with phenol-chloroform-isoamylic acid alcohol (25:24:1, v/v/v, Sigma). It was then again extracted twice with chloroform-isoamylic acid alcohol (24:1, v/v, Sigma). Following treatment with 96% ethanol (Sigma) and centrifugation, the precipitate was re-suspended in distilled water (40 µl) and stored at 4°C till the day of the PCR test [15]. The First Molecular Detection of Caprine Arthritis Encephalitis Virus (Caev) in Iran Gag gene-PCRPrimers used to amplify the group associated antigen (gag) gene were those described by Clavijo and Thorsen [16], their sequence as follows:Forward: 5' AGGAGGAGGATTAACAGTGG-3' Reverse: 5' TCCTGGCCTTAATGCTTGTG-3' PCR amplificationThe PCR reaction was carried out in primus 96 thermal cycler. DNA fragment were amplified in 25 µL reaction mixtures containing approximately 3 µL of genomic DNA, 1.5 mM MgCl 2 , 0.2 mM each Deoxynucleoside Triphosphates (dNTPs), 400 Nm of each primer and 2.5U Taq DNA polymerase and 5% DMSO. The PCR amplification was achieved by 35 cycles each including a denaturation step at 94°C for 1 min, annealing step at 50°C for 30 sec and extension step at 72°C for 1 min. PCR amplified DNA fragments were analyzed by electrophoresis on 1.5% agarose gels stained with ethidium bromide and visualized on a UV trans illuminator [15].

show abstract

Serological survey of caprine arthritis-encephalitis virus in 83 goat herds of Yucatan, Mexico

Cited by 25 publications

References 7 publications

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

Evaluation of Five Enzyme-Linked Immunosorbent Assays and an Agar Gel Immunodiffusion Test for Detection of Antibodies to Small Ruminant Lentiviruses

The First Molecular Detection of Caprine Arthritis Encephalitis Virus (Caev) in Iran

Contact Info

Product

Resources

About