Serum albumins are the major site for in vivo formation of hapten‐carrier protein adducts in plasma from humans and guinea‐pigs exposed to type‐1 allergy inducing hexahydrophthalic anhydride

Abstract

This study shows SA to be the major protein in plasma that forms adducts in vivo with HHPA. The results also show that in an in vitro synthesized HHPA plasma protein conjugate, HHPA-specific IgE and IgG antibodies bind preferably to the SA.

Total plasma protein adducts of allergenic hexahydrophthalic and methylhexahydrophthalic anhydrides as biomarkers of long-term exposure

“…This assumption agrees with the results of other studies (24). This is an interesting finding since conjugates between HSA and anhydrides have been used for many years in tests for specific antibodies.…”

Persulphate challenge in female hairdressers with nasal hyperreactivity suggests immune cell, but no IgE reaction

“…Analysis for speciWc IgE antibodies against persulphates was performed by speciWc immunoblotting (Johannesson et al 2001). Aliquots of the conjugate with persulphate and proteins from nasal lavage Xuid (NLF) were separated by 4-12% SDS-PAGE.…”

Identification and characterisation of adducts between serum albumin and 4,4?-methylenediphenyl diisocyanate (MDI) in human plasma

“…The separation of plasma proteins were performed by ion exchange chromatography (IEC)as previously described by Johannesson et al (2001). Dialysed plasma was diluted with ethanolamine buffer and 500 ll aliquots were injected via a loop into an Ä kta purifier liquid chromatography system (Pharmacia Biotech, Uppsala, Sweden) fitted with a Mono Q HR 5/5 column.…”

“…The separation of plasma proteins were performed as previously described by Johannesson et al (2001). Aliquots of the concentrated samples were injected into the Ä kta purifier liquid chromatography system equipped with a Superdex 200 column (model 10/30; Pharmacia Biotech).…”