Serum levels of IL-32 in patients with coronary artery disease and its relationship with the serum levels of IL-6 and TNF-α

Mohammad-Rezaei, Mina; Ahmadi, Reza; Rafiei, Ali; Khaledifar, Arsalan; Fattahi, Soheila; Samiei-Sefat, Azadeh; Emami, Shohreh; Bagheri, Nader

doi:10.1007/s11033-021-06441-7

Cited by 16 publications

(11 citation statements)

References 21 publications

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“… 24 Furthermore, the result of our recent study showed an increase in the serum concentrations of TNF-α, IL-6, and IL-32 in the CAD group compared with the control group and there was a significant association between the serum TNF-α and IL-32 concentration in the CAD group with the grades of arterial stenosis. 18 The results of this studies showed that serum levels of IL-37 were negatively correlated with the serum levels of IL-6, TNF-α, and IL-32 in patients with CAD.…”

Section: Discussionmentioning

confidence: 67%

“…In another research, we find out that serum levels of TNF-α, IL-6, and IL-32 significantly elevated in CAD group subject compared with control group 18. As shown in figure 5A–C, our results demonstrated that IL-37 had a significant negative correlation with IL-6 (r=−0.429; p=0.011), TNF-α (r=−0.386; p=0.021), and IL-32 (r=−0.408; p=0.016).…”

Section: Resultsmentioning

confidence: 84%

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Serum Levels of IL-37 and Correlation with Inflammatory Cytokines and Clinical Outcomes in Patients with Coronary Artery Disease

Rafiei

Ahmadi

Kazemian

et al. 2022

Journal of Investigative Medicine

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Coronary artery disease (CAD) due to atherosclerosis is one of the important reasons for death worldwide. Recent evidence has suggested the essential role of inflammation in the progression of atherosclerosis. Interleukin (IL)-37 is a critical anti-inflammatory member of the IL-1 family which regulates the inflammatory processes. The aim of this study was to compare the serum levels of IL-37 in patients with CAD compared with the control group and its correlation with oxidative stress, cholesterol homeostasis, and inflammation in patients with CAD. A total of 42 patients with CAD and 42 sex-matched and age- matched controls who underwent coronary angiography were included in this study. The serum levels of IL-37 were evaluated via ELISA. Serum levels of biochemical risk factors were determined by enzymatic methods. Serum levels of IL-37 in the CAD group subjects were significantly lower than in the control group and IL-37 was significantly increased in men with CAD than in women with CAD. IL-37 significantly had an inverse correlation with IL-6, tumor necrosis factor-α, IL-32, high-sensitivity C reactive protein, oxidized low-density lipoprotein, and malondialdehyde. Also, IL-37 had a significantly positive correlation with ferric-reducing antioxidant power (FRAP) assay. In addition, IL-37 has positively correlated with ATP-binding cassette transporter A1 and G1 gene expression in peripheral blood mononuclear cells and serum levels of the FRAP. A receiver operating characteristic test displayed that IL-37 level ratios were a relatively significant CAD predictor. Our results indicated that decreased serum levels of IL-37 in patients with CAD and its relationship with inflammatory cytokines and reverse cholesterol transport genes are more likely to be associated in the inflammatory process with disease pathology.

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Section: Discussionmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 84%

Serum Levels of IL-37 and Correlation with Inflammatory Cytokines and Clinical Outcomes in Patients with Coronary Artery Disease

Rafiei

Ahmadi

Kazemian

et al. 2022

Journal of Investigative Medicine

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“…Furthermore, our recent study showed an increase in the serum levels of TNF-α, IL-6, and IL-32 in the CAD group compared to the control group. There was a significant association between the serum TNF-α and IL-32 concentration in the CAD group with a grade of arterial stenosis [27]. It is hypothesized that IL-36 increases inflammatory cytokines, such as TNF-α, IL-6, and IL-32, in patients with atherosclerosis, which needs to be proven in the related mice model.…”

Section: Discussionmentioning

confidence: 95%

“…In our recent study, the serum levels of IL-32, IL-6, and TNF-α were measured. It was found that when comparing the CAD group to the control group, the serum levels of these cytokines increased 3, 3.32, and 2.55 times, respectively (IL-32, p < 0.0001; IL-6, p < 0.0001; and TNF-α, p = 0.0029) [27].…”

Section: The Serum Levels Of Il-32 Il-6 and Tnf-αmentioning

confidence: 96%

“…In our recent study on the same population, the serum levels of IL-32 were evaluated by an enzyme-linked immunosorbent assay (ELISA) kit (ZellBio GmbH, Germany) with intra-assay coefficients of variability (CV) of <10% and inter-assay CV of <12%. Also, the serum levels of IL-6 and TNF-α were evaluated using an ELISA kit (Carmania Pars Gene, Kerman, Iran) according to the company's instructions with an intra-assay CV of <3% and interassay CV of <8% [27]. The secreted concentrations of total IL-36 in the subjects' serum were measured using the available ELISA kit (ZellBio, GmbH, Veltinerweg, Germany, and Cat.…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

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The Serum Levels of IL-36 in Patients with Coronary Artery Disease and Their Correlation with the Serum Levels of IL-32, IL-6, TNF-α, and Oxidative Stress

Kazemian¹,

Ahmadi²,

Rafiei³

et al. 2022

Int Arch Allergy Immunol

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Introduction: Atherosclerosis is a chronic inflammatory process maintained during all stages of the disease by several proinflammatory mediators, such as cytokines and chemokines. Interleukin (IL)-36 cytokines are proinflammatory and have an essential role in innate and adaptive immunity, but the role of IL-36 has not been determined in coronary artery disease (CAD). This study aimed to measure the serum levels of IL-36 in patients with CAD and their association with the serum levels of tumor necrosis factor (TNF)-α, IL-6, and IL-32 and also investigate their correlation with the serum levels of malondialdehyde (MDA) and ferric reducing antioxidant power (FRAP). Methods: A total of 168 subjects (84 CAD and 84 control subjects) were examined in this research. The total serum levels of IL-36 were measured using the enzyme-linked immunosorbent assay (ELISA). Also, some oxidative stress parameters were evaluated by FRAP and MDA assays in the serum. Results: The serum levels of IL-36 and MDA were significantly higher, and FRAP was significantly lower in the CAD group compared to the controls. Furthermore, the serum levels of IL-36, MDA, and FRAP significantly correlated with the CAD group’s cardiac arterial stenosis. Also, the serum levels of IL-36 had a positive and significant correlation with the serum levels of TNF-α, IL-6, IL-32, and biochemical parameters in the CAD group. Conclusion: Higher serum levels of IL-36 and its association with the serum levels of TNF-α, IL-32, and IL-6 may play a key role in the pathogenesis of CAD, leading to an increased risk of clogged arteries and oxidative stress.

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Orally administered yeast‐derived β‐glucan alleviates mast cell‐dependent airway hyperresponsiveness and inflammation in a murine model of asthma

Zheng,

Bai,

Xia

et al. 2024

Immunity Inflam & Disease

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BackgroundParticulate β‐glucans (WGP) are natural compounds with regulatory roles in various biological processes, including tumorigenesis and inflammatory diseases such as allergic asthma. However, their impact on mast cells (MCs), contributors to airway hyperresponsiveness (AHR) and inflammation in asthma mice, remains unknown.MethodsC57BL/6 mice underwent repeated OVA sensitization without alum, followed by Ovalbumin (OVA) challenge. Mice received daily oral administration of WGP (OAW) at doses of 50 or 150 mg/kg before sensitization and challenge. We assessed airway function, lung histopathology, and pulmonary inflammatory cell composition in the airways, as well as proinflammatory cytokines and chemokines in the bronchoalveolar lavage fluid (BALF).ResultsThe 150 mg/kg OAW treatment mitigated OVA‐induced AHR and airway inflammation, evidenced by reduced airway reactivity to aerosolized methacholine (Mch), diminished inflammatory cell infiltration, and goblet cell hyperplasia in lung tissues. Additionally, OAW hindered the recruitment of inflammatory cells, including MCs and eosinophils, in lung tissues and BALF. OAW treatment attenuated proinflammatory tumor necrosis factor (TNF)‐α and IL‐6 levels in BALF. Notably, OAW significantly downregulated the expression of chemokines CCL3, CCL5, CCL20, CCL22, CXCL9, and CXCL10 in BALF.ConclusionThese results highlight OAW's robust anti‐inflammatory properties, suggesting potential benefits in treating MC‐dependent AHR and allergic inflammation by influencing inflammatory cell infiltration and regulating proinflammatory cytokines and chemokines in the airways.

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Serum levels of IL-32 in patients with coronary artery disease and its relationship with the serum levels of IL-6 and TNF-α

Cited by 16 publications

References 21 publications

Serum Levels of IL-37 and Correlation with Inflammatory Cytokines and Clinical Outcomes in Patients with Coronary Artery Disease

Serum Levels of IL-37 and Correlation with Inflammatory Cytokines and Clinical Outcomes in Patients with Coronary Artery Disease

The Serum Levels of IL-36 in Patients with Coronary Artery Disease and Their Correlation with the Serum Levels of IL-32, IL-6, TNF-α, and Oxidative Stress

Orally administered yeast‐derived β‐glucan alleviates mast cell‐dependent airway hyperresponsiveness and inflammation in a murine model of asthma

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