Severe Chlorotic Spot Symptoms in Cucumber Mosaic Virus Strain Y-Infected Tobaccos Are Induced by a Combination of the Virus Coat Protein Gene and Two Host Recessive Genes

Takahashi, Hideki; Ehara, Yoshio

doi:10.1094/mpmi-6-182

Cited by 44 publications

(31 citation statements)

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“…yielded deduced amino acid sequences for the region from 108 to 161 ; these sequences were identical to those of CMV-M and CMV-P6. The amino acid at position 158 in CMV-O was initially reported to be a Phe (Hayakawa et al, 1989), and later shown to be a Ser (Takahashi & Ehara, 1993). Our culture of CMV-O also had a Ser at this position.…”

Section: Discussionsupporting

confidence: 58%

“…Chlorosis is one of the symptoms induced by CMV in tobacco that has been extensively investigated (Rao & Francki, 1982;Shintaku, 1991;Shintaku et al, 1992;Takahashi & Ehara, 1993). By using classical pseudorecombinant analysis, Rao & Francki (1982) partially mapped the determinant for chlorosis induction by the tripartite virus, CMV-M, to RNA 3.…”

Section: Introductionmentioning

confidence: 99%

“…Fax +81 45 972 6205. reciprocally changes the phenotype from chlorosis to green mosaic or vice versa on tobacco (Shintaku et al, 1992). Induction of severe yellow chlorotic spots on tobacco leaves inoculated with CMV-Y, which is known to be a strain inducing chlorosis on tobacco; has been reported to be associated with the amino acid at position 129 and with two nuclear-coded recessive host genes (Takahashi & Ehara, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco

Suzuki

Kuwata

Masuta

et al. 1995

Journal of General Virology

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco

Suzuki

Kuwata

Masuta

et al. 1995

Journal of General Virology

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“…RNA 4, which is transcribed from the negative strand of RNA 3, serves as a messenger RNA for the viral coat protein (CP). CP is involved in symptom determination in several plants (Shintaku et al, 1992;Takahashi & Ehara, 1993;Suzuki et al, 1995;Takeshita et al, 1998Takeshita et al, , 2001. Another subgenomic RNA, RNA 4A, which is transcribed from RNA2, encodes the 2b protein, which is not necessary for replication (Ding et al, 1994(Ding et al, , 1995.…”

Section: Introductionmentioning

confidence: 99%

Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus

Suzuki

Yoshida

Yoshinuma

et al. 2003

Journal of General Virology

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Cucumber mosaic virus (CMV) and Peanut stunt virus (PSV) each have genomes consisting of three single-stranded RNA molecules: RNA 1, 2 and 3. RNAs 1 and 2 encode the 1a and 2a proteins, respectively, which are necessary for replication of the viral genome. Although RNA 3 is exchangeable between CMV and PSV, exchange of RNA 1 and 2 between the two viruses has been unsuccessful. In this study, reassortants containing PSV RNA 1 and CMV RNA 2 together with RNA 3 of CMV or PSV were shown to be able to replicate their genomic RNA, but not to transcribe subgenomic RNA 4 in tobacco protoplasts. Conversely, the reassortant consisting of CMV RNA 1 and PSV RNA 2 together with RNA 3 of CMV or PSV could not replicate. Subsequently, a yeast two-hybrid system was used to analyse the in vivo interaction between the 1a and 2a proteins. The C-terminal half of PSV-1a protein interacted with the N-terminal region of 2a protein of both PSV and CMV, but the C-terminal half of CMV-1a and the N-terminal region of PSV-2a did not interact. These results suggest that RNA replication in the interspecific reassortant between CMV and PSV requires compatibility between the C-terminal half of the 1a protein and the N-terminal region of the 2a protein, and this compatibility is insufficient for transcription of subgenomic RNA 4. INTRODUCTIONCucumber mosaic virus (CMV) is one of the most important pathogens of many crops, and Peanut stunt virus (PSV) is also an economically important pathogen of legumes worldwide. CMV and PSV are taxonomically related virus species, as shown serologically and by nucleic acid hybridization. They are members of the genus Cucumovirus, together with Tomato aspermy virus (TAV), in the family Bromoviridae, which belongs to the alphavirus-like superfamily (Goldbach, 1987;Koonin & Dolja, 1993). CMV strains are classified into subgroups IA, IB and II (reviewed in Palukaitis et al., 1992;Roossinck et al., 1999). PSV isolates are also classified into two distinct subgroups (Hu et al., 1997).Cucumoviruses have tripartite genomes that consist of three positive-sense, single-stranded RNA molecules, which are designated RNA 1 to 3 in decreasing order of molecular mass. RNA 1 and 2 encode the 1a and 2a proteins, respectively, both of which are necessary for virus replication (Nitta et al., 1988;Hayes & Buck, 1990). RNA 3 encodes the 3a protein, which is involved in virus movement (Suzuki et al., 1991;Canto et al., 1997;Kaplan et al., 1997). RNA 4, which is transcribed from the negative strand of RNA 3, serves as a messenger RNA for the viral coat protein (CP). CP is involved in symptom determination in several plants (Shintaku et al., 1992;Takahashi & Ehara, 1993;Suzuki et al., 1995;Takeshita et al., 1998Takeshita et al., , 2001. Another subgenomic RNA, RNA 4A, which is transcribed from RNA2, encodes the 2b protein, which is not necessary for replication (Ding et al., 1994(Ding et al., , 1995. The RNA 4A of subgroup I CMV was reported to accumulate to a very low level compared with that of subgroup II CMV and TAV (Shi et al., ...

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“…Similar monogenic controls, however recessive, were reported in the case of the symptom development during infection of A. thaliana by Beet curly top virus and Tobacco mosaic virus (Dardick et al 2000;Park et al 2002). On Nicotiana tabacum infected with Cucumber mosaic virus (CMV), the genetic determinism of symptoms was shown to be digenic and recessive (Takahashi and Ehara 1993). Finally, even more complex mechanisms controlling symptom development have been observed, with multiple contributing genes.…”

mentioning

confidence: 99%

Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

Sicard¹,

Keurentjes²,

Candresse³

et al. 2008

MPMI

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In compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. However, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. In a previous study on the Arabidopsis thaliana-Plum pox virus (PPV) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. Two basic types of interaction exist between a virus and its host plant: i) a compatible interaction associated with plant invasion and viral multiplication, followed in most cases by the induction of symptoms, and ii) an incompatible interaction with no or only limited viral multiplication or movement in the host plant. Plant-virus interactions are relatively well documented, but efforts have so far focused on resistance (or resistancebreaking) mechanisms and on the identification of their genetic determinants. Conversely, the one or more mechanisms controlling symptom development in plants are still largely unknown, so that only a very patchy picture of the interaction between a plant and a virus can be presented.

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Severe Chlorotic Spot Symptoms in Cucumber Mosaic Virus Strain Y-Infected Tobaccos Are Induced by a Combination of the Virus Coat Protein Gene and Two Host Recessive Genes

Cited by 44 publications

References 0 publications

Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco

Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco

Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus

Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

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