Short- and long-range Ca2+-induced lateral phase separations in ternary mixtures of phosphatidic acid, phosphatidylcholine and phosphatidylethanolamine

Ohki, Kazuo; Takahashi, Kiyoko; Kato, Satoru; Maesono, Akikazu

doi:10.1016/0009-3084(89)90034-0

Cited by 10 publications

(6 citation statements)

References 31 publications

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“…Besides these studies, the effects of Ca2+ ions on the structural and thermotropic properties of a PA bilayer have been studied. At physiolog-ical pH, in the temperature range between 10 and 100°C, no gel-to-liquid crystalline phase transition has been observed for Ca2+-dimyristoylphosphatidic acid (DMPA) and Ca2+-dipalmitoylphosphatidic acid (DPPA) complexes (Van Dijck et al, 1978;Liao and Prestegard, 1981;Graham et al, 1985;Kouaouci et al, 1985;Ohki et al, 1989;Laroche et al, 1991;Vincent and Levin, 1991). In contrast, the existence of phase transition for the Ca2+-PA complex at acidic pH has been reported by several researchers (Boughriet et al, 1988;Vincent and Levin, 1991).…”

Section: Introductionmentioning

confidence: 98%

“…Thus, many studies on the interaction between PA and calcium ions have already been carried out. For example, it is well known that calcium ions induce lateral phase separation in mixed lipid systems, including PA (Graham et al, 1985;Kouaouci et al, 1985;Eklund et al, 1988;Ohki, 1988;Ohki et al, 1989). Besides these studies, the effects of Ca2+ ions on the structural and thermotropic properties of a PA bilayer have been studied.…”

Section: Introductionmentioning

confidence: 99%

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Structural and thermotropic properties of calcium-dimyristoylphosphatidic acid complexes at acidic and neutral pH conditions

Takahashi

Yasue

Ohki

et al. 1995

Biophysical Journal

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Two kinds of calcium-dimyristoylphosphatidic acid (DMPA) complexes at acidic and neutral pH conditions were prepared in the following ways. The complex at pH 4 was obtained by adding Ca2+ to DMPA dispersion in pure water. On the other hand, the complex at pH 7.4 was obtained by adding Ca2+ to DMPA dispersion in the presence of NaOH. The stoichiometries of Ca2+ ion to DMPA molecule are 0.5-0.67 and approximately 1 for the complexes at pH 4 and 7.4, respectively. Static x-ray diffraction shows that the hydrocarbon chains of the Ca(2+)-DMPA complex at pH 4 at 20 degrees C are more tightly packed than those of the complex at pH 7.4 at 20 degrees C. Furthermore, the complex at pH 4 at 20 degrees C gives rise to several reflections that might be related to the ordered arrangement of the Ca2+ ions. These results indicate that the structure of the complex at pH 4 is crystalline-like. In the differential scanning calorimetry (DSC) thermogram, the complex at pH 7.4 undergoes no phase transition in a temperature range between 30 and 80 degrees C. On the other hand, in the DSC thermogram for the complex at pH 4, a peak appears at 65.8 degrees C in the first heating scan. In the successive second heating scan, a transition peak appears at 63.5 degrees C. In connection with the DSC results, the structural changes associated with these phase transitions were studied with temperature-scan x-ray diffraction. In the first heating scan, although a peak appears at 65.80C in the DSC thermogram, the hydrocarbon chain packing gradually converts from an orthorhombic lattice to a hexagonal lattice near 52 degree C, and successively the chain melting phase transition occurs near 670C. In the second heating scan, the hydrocarbon chains are packed in a hexagonal lattice over the whole temperature range and the chain melting phase transition occurs near 63.5 degree C. Therefore,the Ca2+-DMPA complex at pH 4 has a metastable state. The metastable state transforms to a stable state by maintaining the complex at pH 4 for about 90 h at 200C.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Structural and thermotropic properties of calcium-dimyristoylphosphatidic acid complexes at acidic and neutral pH conditions

Takahashi

Yasue

Ohki

et al. 1995

Biophysical Journal

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“…All preparations contained 5 mM EDTA in order to inhibit phospholipase activity included in melittin [6]. Calorimetric scans were performed with a differential adiabatic micro-calorimeter, Privalov calorimeter DASM-4, at a heating scan rate of 0.5 K min −1 with 0.5 ml volume cells under a pressure of 2.0 atm to prevent bubble formation [7], and the data were processed by an NEC PC-9801RX personal computer (Tokyo, Japan). The heat absorption was calibrated by Jule's heat from the internal equipped circuit.…”

Section: Methodsmentioning

confidence: 99%

“…In the present study, we have investigated the effect of melittin on binary mixtures of phospholipids. Each phospholipid species in membranes has its own feature of phase transition characterized by temperature and enthalpy, so their miscibility, phase transition temperatures and phase transition enthalpies determine the thermogram of the phospholipid mixture [7]. In order to elucidate the preferential interaction between melittin and a phospholipid species, the thermograms of various binary mixtures of phospholipids were analysed in the absence and the presence of melittin.…”

Section: Preferential Interaction Of Melittin With Dimyristoylphosphamentioning

confidence: 99%

Simple Method of Measuring Alignment Directions and Retardation of Liquid Crystal Cells

Nishioka¹,

Satake²,

Maeda³

et al. 2007

jjap

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Using differential scanning calorimetry, preferential interaction between melittin and dimyristoylphosphatidylcholine was observed in various binary mixtures of phospholipids. It is shown that matching of the hydrophobic regions between melittin and fatty acyl chains of phospholipids is the most important factor. Using a microscopic imaging instrument for membrane fluidity, specific interaction between cholesterol and sphingomyelin in rafts was confirmed in living CHO cells. An environment sensitive fluorescence dye, laurdan, was used in this home-built instrument. The membrane fluidity of the cells was scarcely affected with the treatment of sphingomyelinase up to 0.1 U ml −1 . On the other hand, increase of the membrane fluidity was observed in CHO cells treated with methyl-beta-cyclodextrin, which removes cholesterol molecules from biomembranes of the cells in a concentration dependent manner up to 10 mM. But a low concentration of methyl-beta-cyclodextrin (1 mM) did not raise the membrane fluidity. However, increase of membrane fluidity was observed in CHO cells treated with sphingomyelinase and then with 1 mM methyl-beta-cyclodextrin. These results suggest specific interaction between sphingomyelin and cholesterol in the rafts.

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“…Calorimetric measurements were performed with a differential adiabatic microcalorimeter (Privalov calorimeter DASM-4, Sinku Riko, Yokohama, Japan) at a heating scan rate of 0.5 K/min with 0.5-ml-volume cells under a pressure of 2:0 Â 10 3 h Pa to prevent bubble formation. 23) The total lipid concentration was adjusted to 1 mM.…”

Section: Spectroscopic and Calorimetric Measurementsmentioning

confidence: 99%

Microscopic Imaging of Phase Separation in Giant Liposome by Using Laurdan at Video Rate

Ohba

Tsuchiya

Kumeta

et al. 2005

Jpn. J. Appl. Phys.

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Laurdan is a useful fluorescence dye for observation of physical properties of membranes. An optical system was constructed in order to image generalized polarization (GP), that characterizes the change of the spectrum of laurdan. The fluorescence images through the optical system were recorded on a digital video recorder and the images were processed to GP images, which made it possible to measure the spatial distribution of membrane fluidity quantitatively at video rate. Using this imaging instrument, the change of membrane fluidity at every moment of phase transition was observed in a giant liposome of pure dimyristoylphosphatidylcholine and the dynamics of phase separation was observed in a giant liposome composed of dimyristoylphosphatidylcholine and dimyristoylphosphatidylethanolamine after an annealing process. A clear phase separation was observed in the giant liposome in the cooling process from 43 to 40°C. The GP value of the phase-separated region on the liposome was related to the shape of the same region. The linear part of the liposome had a high GP value and the hinge part had a low GP value.

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Short- and long-range Ca2+-induced lateral phase separations in ternary mixtures of phosphatidic acid, phosphatidylcholine and phosphatidylethanolamine

Cited by 10 publications

References 31 publications

Structural and thermotropic properties of calcium-dimyristoylphosphatidic acid complexes at acidic and neutral pH conditions

Structural and thermotropic properties of calcium-dimyristoylphosphatidic acid complexes at acidic and neutral pH conditions

Simple Method of Measuring Alignment Directions and Retardation of Liquid Crystal Cells

Microscopic Imaging of Phase Separation in Giant Liposome by Using Laurdan at Video Rate

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