Signal-to-noise ratio optimization for detecting bacteria with interdigitated microelectrodes

Couniot, Numa; Flandre, Denis; Francis, Laurent; Afzalian, Aryan

doi:10.1016/j.snb.2012.12.008

Cited by 42 publications

(28 citation statements)

References 24 publications

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“…3c) and confirmed by simulations in Fig. S1b (Couniot et al, 2013). In contrast to Δ rt , only the remaining adherent bacteria on the sensor affect Δ w , justifying its smaller value.…”

Section: Shifts and Slopes In Real-timesupporting

confidence: 64%

Lytic enzymes as selectivity means for label-free, microfluidic and impedimetric detection of whole-cell bacteria using ALD-Al2O3 passivated microelectrodes

Couniot

Vanzieleghem

Rasson

et al. 2015

Biosensors and Bioelectronics

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“…3c) and confirmed by simulations in Fig. S1b (Couniot et al, 2013). In contrast to Δ rt , only the remaining adherent bacteria on the sensor affect Δ w , justifying its smaller value.…”

Section: Shifts and Slopes In Real-timesupporting

confidence: 64%

Lytic enzymes as selectivity means for label-free, microfluidic and impedimetric detection of whole-cell bacteria using ALD-Al2O3 passivated microelectrodes

Couniot

Vanzieleghem

Rasson

et al. 2015

Biosensors and Bioelectronics

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“…For the sake of simplicity, a single bacterial cell lies between electrodes in this study (the sensitivity is only slightly reduced when lying atop electrodes). The impact of the number and positions of bacteria between and atop electrodes has been studied in [16]. Chosen as a model for Gram-positive bacteria, Staphylococcus epidermidis features diameter d bact of 1.2 µm.…”

Section: A Frameworkmentioning

confidence: 99%

“…It is known that electrode dimensions must be chosen accordingly to confine the electric field in a layer of similar thickness than target species [14], [15]. Position and orientation of biological species, such as bacteria and DNA strands, have also shown to impact the sensitivity [16], [17]. In addition, the immobilization of bioreceptors has also shown to play a significant role towards N. Couniot a maximal sensitivity [15].…”

Section: Introductionmentioning

confidence: 99%

Capacitive Biosensing of Bacterial Cells: Sensitivity Optimization

Couniot

Afzalian²,

Overstraeten-Schlögel

et al. 2016

IEEE Sensors J.

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Capacitive biosensors are promising tools towards detection of bacterial cells. However, their sensitivity remains insufficient compared to more conventional techniques. To understand how to optimize it, we propose a complete and quantitative analysis of the sensitivity of capacitive biosensors using a previously developed 2D numerical simulator based on Poisson-Nernst-Planck equations. All key parameters of the electrolyte, bacterial cell and electrode design are investigated, studied and optimized. For each, an analytical expression relating the sensitivity to the parameter of interest is given. The guidelines and design tools, provided throughout the manuscript for the designer of capacitive biosensors, are eventually demonstrated by improving the sensitivity of our device under test by a factor of 4. Discussions based on experimental values and analytical models is provided throughout this paper.Index Terms-Bacterial detection, AC impedance spectroscopy, CMOS interdigital capacitor modeling, Metal-Insulator-Electrolyte (MIE), electrical double layer, planar electrodes 1530-437X (c)

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“…The detection limit of 6 ng lL À1 was estimated from the signal-to-noise characteristics of these data (S/N = 3). The signal-to-noise ratio was obtained from the impedimetric variation divided by the standard deviation [41]. The increase in DR CT seemed to present a profile of saturation (Fig.…”

Section: Detection Of Tb Genomic Dna Targetmentioning

confidence: 99%

Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor

Costa

Andrade

Montenegro³

et al. 2014

Journal of Colloid and Interface Science

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In this work, a genosensor for the electrochemical detection of genomic DNA from Mycobacterium tuberculosis was developed. The biosensor is based on self-assembled monolayers of mercaptobenzoic acid (MBA) and magnetite nanoparticles (Fe3O4Nps) on bare gold electrode for immobilization of DNA probe. The aim of this work was the development of a platform based on cysteine-coated magnetic Fe3O4Nps linked via the carboxylate group from MBA to the work electrode surface and subsequently to the DNA probe. The probe-genome interaction was evaluated using a [Fe(CN)6](4-)/[Fe(CN)6](3-) redox pair. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to evaluate the bioelectrochemical behavior of the sensor. Atomic force microscopy images showed Fe3O4Nps immobilized across the electrode surface. The interaction of the sensor with different genome DNA concentrations resulted in changes in the charge transfer resistance, indicating a possible use for tuberculosis detection at low concentrations (detection limit of 6ngμL(-1)).

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Signal-to-noise ratio optimization for detecting bacteria with interdigitated microelectrodes

Cited by 42 publications

References 24 publications

Lytic enzymes as selectivity means for label-free, microfluidic and impedimetric detection of whole-cell bacteria using ALD-Al2O3 passivated microelectrodes

Lytic enzymes as selectivity means for label-free, microfluidic and impedimetric detection of whole-cell bacteria using ALD-Al2O3 passivated microelectrodes

Capacitive Biosensing of Bacterial Cells: Sensitivity Optimization

Self-assembled monolayers of mercaptobenzoic acid and magnetite nanoparticles as an efficient support for development of tuberculosis genosensor

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