Signet ring stomach cancer: Morphological characterization and antigenic profile of a newly established cell line (Mz-Sto-1)

Dippold, Wolfgang; Kron, G.; Boosfeld, Erika; Dienes, Hans‐Peter; Klingel, Reinhard; Knuth, A.; Wagner, R.; Büschenfelde, Karl‐Hermann Meyer zum

doi:10.1016/0277-5379(87)90265-3

Cited by 23 publications

(12 citation statements)

References 14 publications

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“…Published data show very complex changes (Ochi et al, 1986). This cell line shows similarities to the published data with involvement of chromosomes 3, 6 , 7 , 8, 12 and 19 and a missing Y chromosome which, in one study (Ferti-Passantonopoulou et al, 1987), was seen in 3 of 4 male cases. Further studies are required to define which changes (if any) are common for stomach cancer.…”

Section: Discussionsupporting

confidence: 89%

A new gastric carcinoma cell line (LIM1839) derived from a young caucasian male

Whitehead

Novak

Thomas

et al. 1989

Intl Journal of Cancer

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We describe a new and unique gastric carcinoma cell line (LIM1839) derived from a young Caucasian male with rapidly progressing disease. The cell line grows with a pleomorphic morphology and has been in continuous culture for more than 3 years. The cells cannot be cloned in semi-solid agar or grown in nude mice despite numerous attempts. The karyotype of the cultured cells is highly abnormal with a large number of structural and numerical changes. Some chromosomes are dicentric and this feature has persisted in this culture. The cells express one of the small-intestinal dipeptidases, aminopeptidase N, but do not express dipeptidyl peptidase IV or the disaccharidases, sucrase isomaltase or maltase glucoamylase. The cells express high levels of EGF receptors and of messenger RNA for insulin-like growth factor II.

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Section: Discussionsupporting

confidence: 89%

A new gastric carcinoma cell line (LIM1839) derived from a young caucasian male

Whitehead

Novak

Thomas

et al. 1989

Intl Journal of Cancer

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“…Otherwise cells were washed three times with PBS. The staining procedure was performed as described [6]. The percentage of positive cells was estimated visually by phase contrast microscopy.…”

Section: Methodsmentioning

confidence: 99%

New cell lines of gastric and pancreatic cancer: distinct morphology, growth characteristics, expression of epithelial and immunoregulatory antigens

Heike

Röhrig

Gabbert

et al. 1995

Vichows Archiv A Pathol Anat

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Two new cell lines from stomach cancers and one from a pancreatic carcinoma are presented. MZ-GC-1 was established from a hepatic metastasis of a well differentiated gastric adenocarcinoma. MZ-GC-2 was derived from ascites induced by a poorly differentiated gastric adenocarcinoma. MZ-PC-1 originated from the pleural effusion of a moderately well differentiated pancreatic ductal adenocarcinoma. MZ-GC-1 cells were adherent and partially polarized, connected tightly via desmosomes. In contrast MZ-GC-2 cells consisted of slightly adherent or floating subpopulations and displayed no desmosomes. MZ-PC-1 cells were adherent and showed polarized growth, connected by apical junctional complexes. Cell doubling times were 7 days for MZ-GC-1 and 45 h for MZ-GC-2 and MZ-PC-1 cells. MZ-GC-2 and MZ-PC-1 gave rise to nude mouse tumours, resembling the original lesions. Chromosome analysis of the cell lines revealed a high range of numerical abnormalities. Each cell line had cytokeratin patterns fitting well to typical in vivo patterns. Furthermore the cell lines expressed a panel of antigens typical for gastrointestinal epithelia. Unique for MZ-PC-1 were high amounts of secreted Ca19-9. gamma-Interferon enhanced HLA-class I antigens up to twofold and induced ICAM-1 expression on each cell line. HLA-class II antigens were differentially enhanced by gamma-interferon. Due to their distinct characteristics the three tumour cell lines may be useful models in the investigation of the cell biology and immunogenicity of gastrointestinal tumours.

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“…SK-MEL-29 was cloned by limiting dilution, and five clones (SK-MEL-29.1 to -5) were selected for further experiments. The stomach cancer cell line MZ-Sto-1, the hepatoma cell line MZ-Hep-1, and the cell lines of extrahepatic biliary tract cancer, MZ-ChA-1 and SK-ChA-1, have been described (14)(15)(16). AK-EBV is an Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line established from a healthy donor.…”

Section: Methodsmentioning

confidence: 99%

Cytolytic T-cell clones against an autologous human melanoma: specificity study and definition of three antigens by immunoselection.

Knuth

Wölfel

Klehmann

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

162

103

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Cytolytic T-lymphocyte (CTL) clones against an autologous melanoma (SK-MEL-29) were generated by mixed lymphocyte tumor culture and subsequent cloning of responder lymphocytes at limiting dilutions. These CTL clones lysed autologous melanoma but not autologous Epstein-Barr virus-transformed B cells and none of the allogeneic tumor targets included in the specificity analysis. The lysis of autologous melanoma targets could be inhibited by monoclonal antibodies against monomorphic HLA class I determinants. For proliferation of CTLs, the stimulation with the relevant target antigen on autologous tumor cells was essential. Immunoselection experiments carried out with two CTL clones revealed the existence of melanoma subclones that were resistant to lysis by the CTL clones used for immunoselection but were still lysed by other autologous CTL clones. This analysis allowed us to identify three stable simultaneously expressed antigens on the melanoma cells defimed by autologous CTLs.The search for human cancer antigens eliciting a specific autologous immune response has been pursued with serum antibodies and T lymphocytes. The most compelling evidence to date for the existence of tumor antigens eliciting a specific autologous antibody response in patients comes from studies of Old and colleagues (1-3), demonstrating serum autoantibodies that were specific for the autologous cancer cells. Several groups obtained cytolytic T-lymphocyte (CTL) clones that showed specificity for the autologous tumor cells (4-8). We have developed techniques for the systematic production of stable CTL clones directed against autologous melanoma cells (9). In a study of a melanoma patient, MZ-2, three antigens were identified with autologous CTL clones: antigen A was present on all melanoma clones tested, antigen B could be lost during long-term culture, and antigen C was only expressed on a minority of melanoma clones (9).We report here a specificity study of autologous CTL clones obtained against a melanoma SK-MEL-29 from a patient AV. Through immunoselection with CTL clones we were able to define three stable antigens on this melanoma. MATERIALS AND METHODS

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Signet ring stomach cancer: Morphological characterization and antigenic profile of a newly established cell line (Mz-Sto-1)

Cited by 23 publications

References 14 publications

A new gastric carcinoma cell line (LIM1839) derived from a young caucasian male

A new gastric carcinoma cell line (LIM1839) derived from a young caucasian male

New cell lines of gastric and pancreatic cancer: distinct morphology, growth characteristics, expression of epithelial and immunoregulatory antigens

Cytolytic T-cell clones against an autologous human melanoma: specificity study and definition of three antigens by immunoselection.

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