Silencing of &lt;i&gt;Nicotiana benthamiana&lt;/i&gt; SEC14 phospholipid transfer protein reduced jasmonic acid dependent defense against &lt;i&gt;Pseudomonas syringae&lt;/i&gt;

Kiba, Akinori; Imanaka, Yu; Nakano, Masahito; Gàlis, Ivan; Hojo, Yuko; Shinya, Tomonori; Ohnishi, Kouhei; Hikichi, Yasufumi

doi:10.5511/plantbiotechnology.16.0503a

Cited by 7 publications

(2 citation statements)

References 18 publications

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“…We have previously identified the SEC14 gene in N. benthamiana . Suppression of PLC and PLD activities, and production of diacylglycerol (DAG) and PA were observed in NbSEC14- silenced plants, resulting in compromised disease resistance against phytopathogenic bacteria through the jasmonic acid (JA)-dependent pathway ( Kiba et al, 2012 , 2014 , 2016 ). Thus, phospholipid turnover may play an important role in the induction of immune responses in N. benthamiana .…”

Section: Introductionmentioning

confidence: 99%

Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana

Kiba

Nakano

Hosokawa

et al. 2020

Journal of Experimental Botany

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Phospholipid signaling plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here, we isolated two phospholipase C2 (PLC2) orthologs in the N. benthamiana genome, designated as PLC2-1 and 2-2. Both NbPLC2-1 and NbPLC2-2 were expressed in most tissues and were induced by infiltration with bacteria and flg22. NbPLC2-1 and NbPLC2-2 (NbPLC2s) double-silenced plants showed a moderately reduced growth phenotype. The induction of the hypersensitive response was not affected, but bacterial growth and the appearance of bacterial wilt were accelerated in NbPLC2s-silenced plants when they were challenged with a virulent strain of Ralstonia solanacearum that was compatible with N. benthamiana. NbPLC2s-silenced plants showed reduced expression levels of NbPR-4, a marker gene for jasmonic acid signaling, and decreased jasmonic acid and jasmonoyl-L-isoleucine contents after inoculation with R. solanacearum. The induction of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) marker genes was reduced in NbPLC2s-silenced plants after infiltration with R. solanacearum or Pseudomonas fluorescens. Accordingly, the resistance induced by flg22 was compromised in NbPLC2s-silenced plants. In addition, the expression of flg22-induced PTI marker genes, the oxidative burst, stomatal closure, and callose deposition were all reduced in the silenced plants. Thus, NbPLC2s might have important roles in pre- and post-invasive defenses, namely in the induction of PTI.

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Section: Introductionmentioning

confidence: 99%

Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana

Kiba

Nakano

Hosokawa

et al. 2020

Journal of Experimental Botany

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“…Recently, we identified the SEC14 gene, encoding a phospholipid transfer protein, in Nicotiana benthamiana (NbSEC14). The suppression of phospholipase C and D activities and a reduction in the PA content have been observed in NbSEC14-silenced plants, resulting in compromised resistance against phytopathogenic bacteria (Kiba et al 2012(Kiba et al , 2014(Kiba et al , 2016. We also reported that phosphoinositide-specific phospholipase C2 in N. benthamiana participates in basal disease resistance (Kiba et al 2020).…”

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confidence: 65%

Trigalactosyldiacylglycerol 3 protein orthologs are required for basal disease resistance in <i>Nicotiana benthamiana</i>

Tagami

Ohnishi

Hikichi

et al. 2021

Plant Biotechnology

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Phosphatidic acid plays an important role in Nicotiana benthamiana immune responses against phytopathogenic bacteria. We analyzed the contributions of endoplasmic reticulum-derived chloroplast phospholipids, including phosphatidic acid, to the resistance of N. benthamiana against Ralstonia solanacearum. Here, we focused on trigalactosyldiacylglycerol 3 (TGD3) protein as a candidate required for phosphatidic acid signaling. On the basis of Arabidopsis thaliana TGD3 sequences, we identified two putative TGD3 orthologs in the N. benthamiana genome, NbTGD3-1 and NbTGD3-2. To address the role of TGD3s in plant defense responses, we created double NbTGD3-silenced plants using virus-induced gene silencing. The NbTGD3-silenced plants showed a moderately reduced growth phenotype. Bacterial growth and the appearance of bacterial wilt disease were accelerated in NbTGD3-silenced plants, compared with control plants, challenged with R. solanacearum. The NbTGD3-silenced plants showed reduced both expression of allene oxide synthase that encoded jasmonic acid biosynthetic enzyme and NbPR-4, a marker gene for jasmonic acid signaling, after inoculation with R. solanacearum. Thus, NbTGD3-mediated endoplasmic reticulum-chloroplast lipid transport might be required for jasmonic acid signaling-mediated basal disease resistance in N. benthamiana.

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The SEC14 phospholipid transfer protein regulates pathogen-associated molecular pattern-triggered immunity in Nicotiana benthamiana

Kiba

Nakano

Ohnishi

et al. 2018

Plant Physiology and Biochemistry

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Silencing of <i>Nicotiana benthamiana</i> SEC14 phospholipid transfer protein reduced jasmonic acid dependent defense against <i>Pseudomonas syringae</i>

Cited by 7 publications

References 18 publications

Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana

Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana

Trigalactosyldiacylglycerol 3 protein orthologs are required for basal disease resistance in <i>Nicotiana benthamiana</i>

The SEC14 phospholipid transfer protein regulates pathogen-associated molecular pattern-triggered immunity in Nicotiana benthamiana

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