Simultaneous determination of 14-thienyl methylene matrine and matrine in rat plasma by high-performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study

Jiang, Minjie; Wang, Lisheng; Jiang, Weizhe; Huang, Shulin

doi:10.1016/j.jchromb.2014.10.041

Cited by 11 publications

(3 citation statements)

References 16 publications

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“…To investigate the antibiotic-resistance reversal effect of MT, the clinical strain C7 was treated with various concentrations of MT (0, 0.125, 0.25, 2, 8, and 16 μg/mL) for predetermined periods of time (1, 4, 7, 10, and 13 h). The concentrations and incubation time were chosen according to the plasma pharmacokinetic (PK) profiles of MT in rat (Jiang et al, 2015) and human (Zhang et al, 2009). When rat was administered orally at a dose of 10 mg/kg, the peak plasma concentration was 1.44 ± 0.14 μg/mL, and the elimination half-life was 7.80 ± 2.25 h. The corresponding PK parameters were 2.38 ± 0.72 μg/ mL and 7.80 ± 0.70 h after the volunteers received a single oral dose of MT 400 mg soft gelatin capsule.…”

Section: Discussionmentioning

confidence: 99%

Matrine reverses the resistance of Haemophilus parasuis to cefaclor by inhibiting the mutations in penicillin-binding protein genes (ftsI and mrcA)

Zhao,

Yang,

Deng

et al. 2024

Front. Microbiol.

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IntroductionMatrine (MT) is a potential resistance reversal agent. However, it remains unclear whether MT can reverse the resistance of Haemophilus parasuis (H. parasuis) to β-lactams, and, if so, by what mechanism MT works.MethodsWe screened one cefaclor (CEC)-resistant strain (clinical strain C7) from eight clinical (H. parasuis) strains and determined the underlying resistance mechanism. Then, we investigated the reversal effect of MTon the resistance of this strain to CEC.Results and DiscussionThe production of β-lactamase, overexpression of AcrAB-TolC system, and formation of biofilm might not be responsible for the resistance of clinical strain C7 to CEC. Fourteen mutation sites were found in four PBP genes (ftsI, pbp1B, mrcA, and prcS) of clinical strain C7, among which the mutation sites located in ftsI (Y103D and L517R) and mrcA (A639V) genes triggered the resistance to CEC. The minimum inhibitory concentration (MIC) of CEC against clinical strain C7 was reduced by two to eight folds after MT treatment, accompanied by the significant down-regulated expression of mutated ftsI and mrcA genes. Based on such results, we believed that MT could reverse the resistance of H. parasuis to CEC by inhibiting the mutations in ftsI and mrcA genes. Our research would provide useful information for restoring the antimicrobial activity of β-lactams and improving the therapeutic efficacy of Glässer’s disease.

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Section: Discussionmentioning

confidence: 99%

Matrine reverses the resistance of Haemophilus parasuis to cefaclor by inhibiting the mutations in penicillin-binding protein genes (ftsI and mrcA)

Zhao,

Yang,

Deng

et al. 2024

Front. Microbiol.

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“…In addition to being hepatotoxic, the bioavailability of MT is not ideal and has a short in vivo half-life (Jiang et al, 2015b). The pharmacokinetics of MT in rats were studied using the UPLC-MS/MS method, and it was found that at the dose of 2 mg/kg, its absolute oral bioavailability was 17.1 ± 5.4% (Yang et al, 2010).…”

Section: Pharmacokinetics Of Matrinementioning

confidence: 99%

Research progress on the pharmacological effects of matrine

Sun

Cai

et al. 2022

Front. Neurosci.

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Matrine possesses anti-cancer properties, as well as the prevention and treatment of allergic asthma, and protection against cerebral ischemia-reperfusion injury. Its mechanism of action may be (1) regulation of cancer cell invasion, migration, proliferation, and cell cycle to inhibit tumor growth; (2) reduction of oxidized low-density lipoprotein and advanced glycation end products from the source by exerting anti-inflammatory and antioxidant effects; (3) protection of brain damage and cortical neurons by regulating apoptosis; (4) restoration of the intestinal barrier and regulation of the intestinal microbiota. This article aims to explore matrine’s therapeutic potential by summarizing comprehensive information on matrine’s pharmacology, toxicity, and bioavailability.

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“…Although there are many studies on the chemical constituents of “Dogel ebs” , at present, it is essential to obtain its pharmacokinetic parameters and metabolism information to fully understand the pharmacological effects and mechanism of “Dogel ebs.” Unfortunately, the identification of metabolites and illustration of its metabolic patterns have not yet been studied, except the pharmacokinetics investigations on matrine , which restricted the therapeutic applications and development of “Dogel ebs.” In order to clarify the metabolic mechanism of “Dogel ebs,” a target‐group‐change couple with mass defect filtering strategy was proposed based on high performance liquid chromatography–quadrupole exactive Orbitrap mass spectrometry (HPLC‐Q‐Exactive/MS) and Compound Discoverer software. As previously reported , Compound Discoverer software could provide elemental composition assignment, exact mass measurement, and fragmentation information in Traditional Chinese medicines and their metabolites in biological matrices .…”

Section: Introductionmentioning

confidence: 99%

A target‐group‐change couple with mass defect filtering strategy to identify the metabolites of “Dogel ebs” in rats plasma, urine and bile

Dong

et al. 2019

J of Separation Science

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“Dogel ebs” was known as Sophora flavescens Ait., a classical traditional Chinese Mongolian herbal medicine, which had the effects on damp‐heat dysentery, scrofula, and syndrome of accumulated dampness toxicity. Although the chemical constituents have been clarified by our previous studies, the metabolic transformation of “Dogel ebs” in vivo was still unclear. To explore the mechanism of “Dogel ebs,” the metabolites in plasma, bile, and urine samples were investigated. A fast positive and negative ion switching technology was used for the simultaneous determination of flavonoids and alkaloids in “Dogel ebs” in a single run. And a target‐group‐change coupled with mass defect filtering strategy was utilized to analyze the collected data. 89 parent compounds and 82 metabolites were characterized by high‐performance liquid chromatography with quadrupole exactive Orbitrap mass spectrometry. Both phase I and phase II metabolites were observed and the metabolic pathways involved in oxidation, demethylation, acetylation, and glucuronidation. 69 metabolites of “Dogel ebs,” including three hydroxyls bonding xanthohumol, formononetin‐7‐O‐glucuronide, 2′‐hydroxyl‐isoxanthohumol decarboxylation metabolite, oxysophocarpine dehydrogen, 9α‐hydroxysophoramine‐O‐glucuronide, etc. were reported for the first time.

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Simultaneous determination of 14-thienyl methylene matrine and matrine in rat plasma by high-performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study

Cited by 11 publications

References 16 publications

Matrine reverses the resistance of Haemophilus parasuis to cefaclor by inhibiting the mutations in penicillin-binding protein genes (ftsI and mrcA)

Matrine reverses the resistance of Haemophilus parasuis to cefaclor by inhibiting the mutations in penicillin-binding protein genes (ftsI and mrcA)

Research progress on the pharmacological effects of matrine

A target‐group‐change couple with mass defect filtering strategy to identify the metabolites of “Dogel ebs” in rats plasma, urine and bile

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