Simultaneous determination of quercetin and its glycosides from the leaves of Nelumbo nucifera by reversed-phase high-performance liquid chromatography

Goo, Hyun Ryul; Choi, Jae Sue; Na, Dong Hee

doi:10.1007/s12272-009-1136-y

Cited by 32 publications

(20 citation statements)

References 15 publications

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“…In a phytochemical study, the simultaneous determination of active components is critical to ensure the quality of plant materials and to find key components manifesting pharmacological effects (Goo et al, 2009). In this study, HPLC conditions for separating phlorotannins were optimized after several trials with mobile phases of acetonitrile-water or methanol-water containing formic acid in various proportions.…”

Section: Resultsmentioning

confidence: 99%

Quantitative determination of major phlorotannins in Ecklonia stolonifera

Goo

Choi

2010

Arch. Pharm. Res.

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Ecklonia stolonifera is a rich source of phlorotannins, which are responsible for the potent pharmacological effects associated with this seaweed. The purpose of this study was to develop a reversed-phase high-performance liquid chromatography method for the simultaneous determination of three major phlorotannins, eckol, dieckol, and phlorofucofuroeckol-A, in the extracts of Ecklonia stolonifera. The optimal chromatographic conditions were achieved on a Thermo Hypersil Gold C-18 column (250 x 4.6 mm i.d., 5 microm) using linear gradient elution of acetonitrile and water containing 0.1% formic acid at UV 254 nm. The separated phlorotannins were identified by liquid chromatography-mass spectrometry. The high-performance liquid chromatography method showed good linearity (r2 > 0.998), precision (1.4-9.5%), and accuracy (93.9-108.7%). The limits of detection ranged from 0.06 to 0.30 microg/mL and the lower limits of quantitation ranged from 0.2 to 1.0 microg/mL. Among phlorotannins, dieckol was the most abundant in both ethanol and ethyl acetate extracts of Ecklonia stolonifera.

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Section: Resultsmentioning

confidence: 99%

Quantitative determination of major phlorotannins in Ecklonia stolonifera

Goo

Choi

2010

Arch. Pharm. Res.

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“…The identification of polyphenolic compounds was carried out by comparing their retention times and mass spectra with the authentic standards, where available. The remaining unidentifiable compounds were searched and verified by referring to the data obtained from the literature that had been supported by sufficient UV–visible information and mass spectra , , . Triplicate determinations were performed.…”

Section: Methodsmentioning

confidence: 99%

Unique bioactive polyphenolic profile of guava (Psidium guajava) budding leaf tea is related to plant biochemistry of budding leaves in early dawn

et al. 2012

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“…Quercetin and baicalein (Supplementary data -S1) are both bioflavonoids that are lipophilic. They are the most common polyphenolic compounds in nature and are found ubiquitously in plants, including food products like onions, many fruits, or in herbs [32][33][34][35]. Previous studies have shown that quercetin and baicalein can act as competitive inhibitors for the signalling compound towards LasR/Rh1R and orphan regulator QscR receptors pathway commonly found with P. aeruginosa and can serve as novel QS-based antivirulence agents to manage such groups of pathogens [19, 36 -38].…”

Section: Introductionmentioning

confidence: 99%

Chitosan nanoencapsulation of flavonoids enhances their quorum sensing and biofilm formation inhibitory activities against an E.coli Top 10 biosensor

Omwenga

Hensel

Shitandi

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Phytochemicals have been found to be promising alternatives to conventional antibiotic therapies for the control of bacterial infections, as they may entail less selective pressure and hence reduce the development of resistance. This study involved examining the inhibition of biofilm formation and of quorum sensing (QS), and the cytotoxicity on mammalian cells of two flavonoids, quercetin and baicalein, in free form and associated into chitosan-based nanocapsules. This was done by use of a transformed E. coli Top 10 biosensor strain, while the cytotoxicity was evaluated on MDCK-C7 cells. In free form, application both flavonoids exhibited slight inhibitory activity on the QS response and biofilm formation, a scenario that was improved positively upon encapsulation with chitosan (Mw ∼115,000 g/mol and DA ∼42%). The association efficiency of 99% (quercetin) and 87% (baicalein) was determined, and each formulation had an average diameter of 190 ± 4 and 187 ± 2 nm, and zeta (ζ) potential of +48.1 ± 2.03 and +48.4 ± 3.46 mV, respectively. Both types of systems were stable against aggregation in M9 and MEM media. The in vitro release kinetics data of both flavonoids seemed to be similar with only ∼20% released over the first 5 h, or ∼10% over the first 4 h, respectively, with subsequent sudden release increase up to ∼40% in both cases. The free phytochemicals seemed to be cytotoxic to MDCK-C7 cells at higher doses, however, upon nanoencapsulation, a cytoprotective effect was evidenced. We have gained proof-of-principle of the advantages of encapsulation of two bioactive flavonoids.

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Simultaneous determination of quercetin and its glycosides from the leaves of Nelumbo nucifera by reversed-phase high-performance liquid chromatography

Cited by 32 publications

References 15 publications

Quantitative determination of major phlorotannins in Ecklonia stolonifera

Quantitative determination of major phlorotannins in Ecklonia stolonifera

Unique bioactive polyphenolic profile of guava (Psidium guajava) budding leaf tea is related to plant biochemistry of budding leaves in early dawn

Chitosan nanoencapsulation of flavonoids enhances their quorum sensing and biofilm formation inhibitory activities against an E.coli Top 10 biosensor

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