Simultaneous Determination of Tetracycline, Macrolide and Sulfonamide Antibiotics in Soils Using Accelerated Solvent Extraction Followed by Solid-Phase Extraction and High Performance Liquid Chromatography Tandem Mass Spectrometry

Abstract

This research developed and optimized the methods for simultaneous determination of tetracycline (TCs), macrolide (MLs) and sulfonamide (SAs) antibiotics in soils using accelerated solvent extraction (ASE)- solid-phase extraction (SPE)-high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Methanol-citric acid (pH=4.7) was used as extraction solvent, diatomite (washed by EDTA) was used as dispersing agent. Firstly, soil was extracted by ASE with the parameter conditions: pressure 1500 psi, temperature 70°C, static 10 min, 1 circle, then pre-concentration by SPE and followed by HPLC-MS/MS analysis. Recovery was 86.3%~97.4% for SAs, 67.3%~87.4% for TCs and 68.4%~78.3% for MLs. RSD < 9 % and r > 0.99. Limits of detection (LOD) was 0.5~0.9 ug/kg for SAs, 0.2~1.1 ug/kg for TCs and 0.2~0.3 ug/kg for MLs. This method determined 9 kinds of antibiotics within 15 min, the determination accuracy can meet the requirements of actual analysis.

Development of a Quantitative Method for Detection of Multiclass Veterinary Drugs in Feed Using Modified QuPPe Extraction and LC–MS/MS

“…Methanol, acetone, acetonitrile, dichloromethane, and ethyl acetate are solvents commonly used for the extraction of various pesticide and veterinary drugs, of which methanol is the solvent most used to dissolve and extract polar and nonpolar chemicals. In many previous studies, methanol has been widely used in the extraction of veterinary drugs, such as tetracyclines, macrolides, and sulfonamide antibiotics [ 14 , 26 , 27 , 28 , 29 , 30 ]. Extraction conditions are mainly associated with the physical and chemical features of the target compounds as well as the features of the solvents.…”

Simultaneous Extraction of Four Antibiotic Compounds from Soil and Water Matrices

“…A wide variety of methods have been employed to determine antibiotic compounds from soil and water samples. The methods utilized most frequently for soil extraction are liquid-solid extraction (LSE) [11,12], ultrasonic assisted extraction (UAE) [13][14][15], accelerated solvent extraction (ASE) [16][17][18], and pressurized liquid extraction (PLE) [19,20]. Sample clean up and concentration of water samples and soil extracts typically occurs via solid phase extraction (SPE) [18,21,22].…”

“…The methods utilized most frequently for soil extraction are liquid-solid extraction (LSE) [11,12], ultrasonic assisted extraction (UAE) [13][14][15], accelerated solvent extraction (ASE) [16][17][18], and pressurized liquid extraction (PLE) [19,20]. Sample clean up and concentration of water samples and soil extracts typically occurs via solid phase extraction (SPE) [18,21,22]. Liquid chromatography with tandem mass spectrometry (LC/MS-MS) is commonly used to determine the analytes of interest [11,13,14,18,20].…”

“…Sample clean up and concentration of water samples and soil extracts typically occurs via solid phase extraction (SPE) [18,21,22]. Liquid chromatography with tandem mass spectrometry (LC/MS-MS) is commonly used to determine the analytes of interest [11,13,14,18,20]. To date, the only standard methodology available for the simultaneous extraction of antibiotics is EPA Method 1694 [23].…”

Simultaneous determination of 13 sulfonamides at trace levels in soil by modified QuEChERS with HPLC-MS/MS

“…Sample pretreatment is an important process for enhancing the sensitivity and accuracy of analytical methods. In previous studies, various methods have been proposed, such as solid-phase extraction, 18–21 QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe), 22 and matrix solid-phase dispersion extraction (MSPD). 23 Among them, QuEChERS was considered as a preferred procedure because of its simplicity, rapidity, and environmental friendliness.…”