Simultaneous determination of water-soluble vitamins in beverages and dietary supplements by LC-MS/MS

Abstract

An LC-MS/MS method was developed for the simultaneous determination of 15 water-soluble vitamins that are widely used as additives in beverages and dietary supplements. This combined method involves the following simple pre-treatment procedures: dietary supplement samples were prepared by centrifugation and filtration after an extraction step, whereas beverage samples were diluted prior to injection. Chromatographic analysis in this method utilised a multi-mode ODS column, which provided reverse-phase, anion- and cation-exchange capacities, and therefore improved the retention of highly polar analytes such as water-soluble vitamins. Additionally, the multi-mode ODS column did not require adding ion pair reagents to the mobile phase. We optimised the chromatographic separation of 15 water-soluble vitamins by adjusting the mobile phase pH and the organic solvent. We also conducted an analysis of a NIST Standard Reference Material (SRM 3280 Multi-vitamin/Multi-element tablets) using this method to verify its accuracy. In addition, the method was applied to identify the vitamins in commercial beverages and dietary supplements. By comparing results with the label values and results obtained by official methods, it was concluded that the method could be used for quality control and to compose nutrition labels for vitamin-enriched products.

The efficiency of ultrasonic-assisted extraction of cyanocobalamin is greater than heat extraction

“…Infusion is a process that takes advantage of boiling temperature to release cyanocobalamin into the extraction solvent(s), while maceration allows soaking at certain conditions to release the compound of interest [7]. Different techniques have been documented in the literature to extract cyanocobalamin from ground dietary supplements, such as sample preparation in a 65 C water bath for 10 min [8], using a rotary shaker [9] and in an ultrasonic water bath [10]. Ultrasound-assisted extraction (UAE) has been commonly practised in analytical chemistry for sample preparation using either an ultrasonic bath or a probe-based system.…”

The Importance of Reference Materials and Method Validation for Advancing Research on the Health Effects of Dietary Supplements and Other Natural Products

“…Van Berkel and coworkers used SRM 3280 in method development/validation studies for rapid and high-throughput determination of vitamins B 1 , B 2 , B 3 , B 5 , and B 6 ( 56 ) and ascorbic acid and folic acid ( 59 ) using flow injection MS/MS without chromatographic separation and demonstrated good agreement with the certified values. Kakitani et al validated an LC-MS/MS method for 15 water-soluble vitamins in dietary supplements and beverages by comparing results from the analysis of SRM 3280 ( 66 ). Chen et al developed a single-laboratory validated method using LC with three different detection modes, i.e., diode array detection (DAD), fluorescence detection (FLD), and MS for the determination of seven B vitamins.…”

Liquid Chromatography Analysis of Common Nutritional Components, in Feed and Food

“…As an excellent example of hydrosoluble vitamins analysis using LC-MS/MS in the food industry, is the determination of 15 compounds in beverages using a multi-mode column (SM-C 18 column, 150 × 2.0 mm, 3 μm; Imtakt Co., Kyoto, Japan), which provided reverse-phase, anion- and cation-exchange capacities, and therefore improved the retention of highly polar analytes such as water-soluble vitamins. The use of this column removes the need for an ion pair reagent in the mobile phase [385]. Finally, we encourage the reader toward a recent and ample review regarding fat- and hydrosoluble vitamins, respectively [386,387].…”