Sintesis Dan Uji Porositas Scaffold Hidroksiapatit/Alginat

Milla, Lalita El; Indrani, Decky Joesiana; Irawan, Bambang

doi:10.30659/odj.5.1.49-53

Cited by 6 publications

(5 citation statements)

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“…During the freeze-drying process, the water contained will sublimate and produce a larger pore size and porosity. The results of this study are following other studies that stated an increase in the percentage of porosity due to the reduction in the concentration of alginate as a polymer [27]. The porosity, which is suitable for application in the tissue engineering field, is around 50-90%.…”

Section: Discussionsupporting

confidence: 84%

The Characteristics, Swelling Ratio and Water Content Percentage of Chitosan-gelatin/limestone-based Carbonate Hydroxyapatite Composite Scaffold

Rianti

Fanny

Nathania

et al. 2022

IJIE

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The tissue engineering field has developed a scaffold that can be used to increase the bone regeneration process. Carbonate hydroxyapatite (CHA) is a well-known scaffold due to its human bones resembling components. The scaffold was synthesized from K, G, and limestone-based CHA using a freeze-drying method with K-G/CHA ratios (w/w) of 40:60, 30:70, 20:80, and 10:90. A Fourier transform infrared spectroscopy (FTIR), a scanning electron microscope-energy dispersive X-ray (SEM-EDX), and X-ray diffraction (XRD) were used to characterize the scaffold. The FTIR test showed some functional groups, such as hydroxyl, amide I, amide II, carbonate, and phosphate. The SEM-EDX test showed micropore (<50 um) and macropores (>50 um) structures as well as elements of C, N, O, Mg, Al, Si, P, and Ca. The XRD analysis obtained crystalline and amorphous particles. The water content percentage (WCP) values obtained were 61.29%, 64.30%, 67.71%, and 67.78%. The K-G/CHA composite scaffold with a ratio of 30:70 has ideal characteristics, a swelling ratio, and a water content percentage.

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Section: Discussionsupporting

confidence: 84%

The Characteristics, Swelling Ratio and Water Content Percentage of Chitosan-gelatin/limestone-based Carbonate Hydroxyapatite Composite Scaffold

Rianti

Fanny

Nathania

et al. 2022

IJIE

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“…The ideal porosity percentage for bone healing is in the range of 80%-90%. This percentage of porosity will cause a larger surface area for bio-material, which is more favourable for early cell attachment [23,46].…”

Section: Discussionmentioning

confidence: 99%

“…Material characterisation provides information about the physical and chemical properties of the material that can be influenced by the size and components [19]. In this study, a characterisation test will be carried out using Fourier transform infrared (FTIR) to analyse functional groups of bio-materials [20]; X-ray diffraction (XRD) to study the crystal structure of a material by using X-rays [21]; scanning electron microscope (SEM) to analyse the shape of the surface, topography, morphology and composition of a material [22]; and porosity analysis to study the pore structure of materials that are suitable for cells to grow and develop [23].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of Bovine Amniotic Membrane with Hydroxyapatite Bio-Composite

et al. 2022

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The fabrication of bio-composite-derived bovine amniotic membrane (BAM) with hydroxyapatite (HAp) is an approach to combining organic and inorganic bio-material to improve the properties of both materials. This research aims to combine, fabricate and characterise the bio-composite of BAM–HA. The combination of bio-composite is made from BAM and HAp in a ratio of 30:70, 35:65, and 40:60. Dried BAM is immersed in saline and then blended until it forms an amniotic slurry with a jelly-like consistency. At this stage, HAp is added so that it can bind to BAM. After the mixture is homogeneous, the freeze-drying process is carried out. After fabrication, all the bio-composites were characterised using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and porosity analysis, and biological activity was conducted using fibroblasts. The bio-composite has functional groups of amides I, II, III, A, B, OH, CO32− and PO43− according to the results of the FTIR. The XRD analysis showed the presence of HAP crystals. This functional group and the crystal HAP indicate that these two materials are bound. An SEM examination revealed a variety of porous patterns on the surface area. The bio-composite with BAM and HAp at a ratio of 35:65 has a higher mean pore size of 155.625 µm with mean porosity of 89.23% and can maintain the fibroblast viability of 95.14%. In conclusion, the study successfully combined both bio-materials BAM and HAp, which have potential synergistic effects on soft and hard tissue regeneration. The ratio of 35:65 showed good characteristics and was non-toxic.

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“…al. stated that a lower concentration of alginate generated lower viscosity because of less PBS consumption, which sublimated easily in the freeze-drying method [18].…”

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Hydroxyapatite/Zinc Composites with Alginate for Bone Scaffold Application

Ramadhani

Tajalla

Parmita

et al. 2023

Advances in Science and Technology

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Studies on hydroxyapatite are plentiful since the compounds are alike to the bone and sometimes combined with Zn to fabricate a longstanding structure and improve cell regeneration. However, construct a 3D model of HAp-Zn through sintering required high energy. Therefore, this study performed a low-temperature process using alginate, taking a role as a matrix, to achieve an interconnected porous structure while maintaining mechanical properties. The variations of alginate concentrations (0%, 2%, 4%, and 6%) were mixed with HAp-Zn powder using PBS and CaCl2. The bone scaffold was then characterized using XRD, FTIR, SEM, and compressive test. The XRD result showed that alginate addition did not change the HAp-Zn structure and phase. The degree of crystallinity and crystallite size was found to decrease with the increasing composition of alginate. The FTIR spectra also displayed an intermolecular interaction between HAp-Zn and SA. On the other hand, the SEM observation contained well interconnectivity with 50-200 μm of pore size. It led to an incline in ultimate compressive strength and modulus of elasticity with the uppermost value was 2.05 ± 0.06 MPa and 0.037 ± 0.001 GPa respectively in 6% of alginate.

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Sintesis Dan Uji Porositas Scaffold Hidroksiapatit/Alginat

Cited by 6 publications

References 0 publications

The Characteristics, Swelling Ratio and Water Content Percentage of Chitosan-gelatin/limestone-based Carbonate Hydroxyapatite Composite Scaffold

The Characteristics, Swelling Ratio and Water Content Percentage of Chitosan-gelatin/limestone-based Carbonate Hydroxyapatite Composite Scaffold

Characterisation of Bovine Amniotic Membrane with Hydroxyapatite Bio-Composite

Preparation and Characterization of Hydroxyapatite/Zinc Composites with Alginate for Bone Scaffold Application

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