Size Reduction of Dried Pellets Containing <i>Serratia marcescens</i>

Pfeifer, V. F.; Vojnovich, C.; Maister, H. G.; Heger, E. N.; Bogart, W. M.

doi:10.1021/ie50580a034

Cited by 3 publications

(3 citation statements)

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“…Size Reduction of Dried Pellets. Details of grinding studies carried out in a ball mill, a fluid energy mill, and a high-speed centrifugal mill with dried pellets containing V. marcescens have been reported (9); only a summation of the findings is given here.…”

Section: Resultsmentioning

confidence: 99%

Pilot-Plant Production of Ground Serratia marcescens

Pfeifer¹,

Vojnovich²,

Maister³

et al. 1958

Ind. Eng. Chem.

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Section: Resultsmentioning

confidence: 99%

Pilot-Plant Production of Ground Serratia marcescens

Pfeifer¹,

Vojnovich²,

Maister³

et al. 1958

Ind. Eng. Chem.

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“…Storage of ground material vs. whole pellets. To test the relative survival of ground material as compared with that of whole pellets, a considerable number of samples were reduced to fine powders in the Spinco10 mill as described by Pfeifer et al (1958) and, subsequently, were stored at 50 C. Originally these samples were stored under vacuum but, wjhen storage under nitrogen was found to give equally good results, its routine use was adopted, since it was difficult to obtain a vacuumtight seal on the straight-neck tubes containing powder. Results of 24 experiments with dried pellets and powders prepared from them gave the following average ST50 values at 50 C: dried pellets, 6.7 weeks; powders, 3.8 weeks.…”

Section: Storage Of S Marcescehnsmentioning

confidence: 99%

“…Storage tests were conducted on cells of the organism in the form of dried pellets or powders; the latter were obtained by grinding dried pellets in various milling devices (Pfeifer et al, 1958). Samples of these materials were sealed in Pyrex glass tubes under reduced pressure (40 ,u of mercury) or under atmospheres of dry air, oxygen, nitrogen, or helium at 760 mm of mercury.…”

mentioning

confidence: 99%

Survival During Storage of Serratia marcescens Dried by Continuous Vacuum Sublimation1

Maister¹,

Pfeifer²,

Bogart³

et al. 1958

Applied Microbiology

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An investigation was made of the various factors affecting survival during storage of Serratia marcescens strain 8 UK after freeze-drying by a continuous vacuum sublimation method. Rtogers (1914), Heller (1941), Fry and Greaves (1951), and Proom and Hemmons (1949) reported on environmental conditions affecting the storage survival of various types of bacteria; Naylor and Smith (1946) made a similar study of S. marcescens. Fry (1954) summarized the factors affecting survival as: 1. Nature of the organism. 2. Suspending medium. 3. Conditions of growth and age of culture. 4. Cell concentration of the suspension. 5. Degree of vacuum and drying temperature. 6. Atmosphere of storage. 7. Temperature of storage. 8. Residual moisture content. However, Fry does not distinguish between those factors affecting survival during drying aind those affecting survival during storage. Our objective was to determine as accurately as possible those conditions most favorable to the storage survival of a strain of S. marcescens. The variables investigated were: residual moisture content, age of culture, control of pH during growth of the organism, concentration of cell suspension, atmosphere of storage, storage of whole pellets vs. ground powder, additioni of glucose to the medium at the end of the usual sugarutilization cycle, and temperature of storage. MATERIALS AND MIETHODS The organisms used in our investigation.s wvere propagated by methods described by Benedict et al. (1957) on a medium consisting of an aqueous mixture of 5 per cent dried skim milk solids, 3 per cent Wilson's protopeptone no. 343,5 and 2.2 per cent glucose (mono-1 This work was supported by a contract with the Chemical Corps,

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