Small-Diameter Subchondral Drilling Improves DNA and Proteoglycan Content of the Cartilaginous Repair Tissue in a Large Animal Model of a Full-Thickness Chondral Defect

Abstract

This study quantified changes in the DNA content and extracellular matrix composition of both the cartilaginous repair tissue and the adjacent cartilage in a large animal model of a chondral defect treated by subchondral drilling. Content of DNA, proteoglycans, and Type II and Type I collagen, as well as their different ratios were assessed at 6 months in vivo after treatment of full-thickness cartilage defects in the femoral trochlea of adult sheep with six subchondral drill holes, each of either 1.0 mm or 1.8 mm in diameter by biochemical analyses of the repair tissue and the adjacent cartilage and compared with the original cartilage. Only subchondral drilling which were 1.0 mm in diameter significantly increased both DNA and proteoglycan content of the repair tissue compared to the original cartilage. DNA content correlated with the proteoglycan and Type II collagen content within the repair tissue. Significantly higher amounts of Type I collagen within the repair tissue and significantly increased DNA, proteoglycan, and Type I collagen content in the adjacent cartilage were identified. These translational data support the use of small-diameter bone-cutting devices for marrow stimulation. Signs of early degeneration were present within the cartilaginous repair tissue and the adjacent cartilage.

rAAV-Mediated Human FGF-2 Gene Therapy Enhances Osteochondral Repair in a Clinically Relevant Large Animal Model Over Time In Vivo

“…14,25 Although histological scoring represents the gold standard of cartilage repair assessment, 47,48 these data support the concept of complementary evaluations of both the biochemical composition and histological structure of the repair tissue. 43,48 Similarly, type I collagen deposition was significantly decreased, mirroring reduced degeneration, in good agreement with previous work. 14,25 Taken together, the sustained expression of FGF-2 possibly triggered multiple reparative and protective cascades by sequentially releasing growth and other factors, inducing a more permanent osteochondral repair phenotype.…”

Decellularized Wharton Jelly Implants Do Not Trigger Collagen and Cartilaginous Tissue Production in Tracheal Injury in Rabbits

“…The trachea is a complex system that provides tissue support and maintains cell-cell interactions, cell-matrix interactions, cell differentiation, and tissue organization. Despite all efforts, repair of the defect in cartilaginous tissues is unsatisfactory due to the avascular nature and limited regenerative capacity of this cartilage [ 18 ]. Several methods have been proposed to replace a defective trachea [ 1 , 16 , 19 , 20 , 21 , 22 ].…”

Repair of articular cartilage defect using adipose‐derived stem cell‐loaded scaffold derived from native cartilage extracellular matrix

“…Histological evaluation mainly included the evaluation of major components of chondro‐extracellular matrix, mainly including Type II collagen and proteoglycan (Orth, Eldracher, Cucchiarini, & Madry, 2020). Type II collagen is mainly used for immunohistochemical staining and indirect reaction content.…”