Soluble Neuropilin-1 in gingival crevicular fluid from periodontitis patients: An exploratory cross-sectional study

Prieto, Diego; G, Maurer; Sáez, Maximiliano; Cáceres, F.; Pino‐Lagos, Karina; Chaparro, Alejandra

doi:10.1016/j.jobcr.2020.11.015

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…Sema3A was found to be significantly elevated in groups P and G than group H. Studies around implants 37 and teeth 38 reported increased gene expression of Sema3A in peri‐implantitis sites 37 and increased GCF Sema3A receptor (neuropilin‐1; Nrp‐1) levels in periodontitis stages III and IV. Increased GCF Nrp‐1 levels were also reported in RA patients, compared with controls 39 .…”

Section: Discussionmentioning

confidence: 93%

Gingival crevicular fluid lipocalin‐2 and semaphorin3A in stage III periodontitis: Non‐surgical periodontal treatment effects

Ceylan

Yılmaz

Narin

et al. 2022

J of Periodontal Research

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Background and Objective Identification of biomarkers to assess individual risk and monitor periodontal health status is important. Research on lipocalin‐2 (LCN2) and semaphorin3A (Sema3A) is lacking. This study aimed to evaluate gingival crevicular fluid (GCF) LCN2, Sema3A, and tumor necrosis factor‐α (TNF‐α) levels in periodontally healthy (H), gingivitis (G), and periodontitis (P) patients, and their changes following non‐surgical periodontal therapy. Methods Sixty systemically healthy and non‐smoker participants, diagnosed as periodontally healthy, gingivitis, and stage III grade C periodontitis, were recruited (n = 20/group). Clinical periodontal parameters were recorded and GCF samples were obtained at baseline from all groups; for group P, these were repeated one and three months following non‐surgical periodontal treatment. GCF LCN2, Sema3A, and TNF‐α levels were evaluated with enzyme‐linked immunosorbent assay. Results GCF LCN2, Sema3A, and TNF‐α total amounts were significantly higher in disease groups than group H (p < .001). Between P and G groups, only TNF‐α levels were significantly different (p < .001). Non‐surgical periodontal therapy resulted in significant improvement of all clinical parameters and significant decreases of GCF LCN2 and TNF‐α levels, at both time points, compared with baseline (p < .001). Sema3A levels remained unchanged following treatment (p > .05). LCN2 and TNF‐α levels were significantly positively correlated with clinical parameters. LCN2 (AUC [area under the curve] = 0.94) and TNF‐α (AUC = 0.98) levels were similarly accurate in differentiating between periodontal disease (whether G or P) and healthy controls. Conclusions LCN2 and TNF‐α levels in GCF are correlated with clinical parameters and could prove useful as non‐invasive screening tools for periodontitis.

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Section: Discussionmentioning

confidence: 93%

Gingival crevicular fluid lipocalin‐2 and semaphorin3A in stage III periodontitis: Non‐surgical periodontal treatment effects

Ceylan

Yılmaz

Narin

et al. 2022

J of Periodontal Research

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“…A direct link between CP and OS-related bio- marker levels in the local site has been suggested by a significant decrease of total antioxidant capacity and a significant increase of malondialdehyde (MDA), nitric oxide, total oxidant status (TOS), and 8-hydroxy-de-oxyguanosine levels in the saliva of CP patients [ 100 ]; MicroRNAs (MiRNA-146a and miRNA155) provide consistent, non-invasive, diagnostic and prognostic biomarkers that can be used to monitor periodontal health status in saliva among diabetic and non-diabetic patients [ 101 ]; Salivary oxidative stress biomarkers and advanced glycation end products were investigated in a cross-sectional study in patients affected by periodontitis and in periodontally healthy patients with type 2 diabetes and corresponding systemically healthy controls. Salivary 8-hydroxy-2’-deoxyguanosine (8-OHdG) alone, or in combination with 4-hydroxy-2-nonenal (4-HNE), advanced glycation end products (AGE) and AGE receptor (RAGE) for diabetics, and salivary 8-OHdG alone, or in combination with malondialdehyde (MDA) and high sensitivity C-reactive protein (hsCRP) for systemically healthy persons, could potentially serve as non-invasive screening marker(s) of periodontitis [ 102 ]; Soluble Neuropilin-1 (sNRP-1) is a glycoprotein with angiogenic and immune regulatory functions positively related to periodontitis and could probably be involved in the pro-inflammatory mechanisms observed in periodontal clinical tissue inflammation [ 103 ]. …”

Section: The Most Promising Host Derived Biomarkers As Candidates mentioning

confidence: 99%

“…Soluble Neuropilin-1 (sNRP-1) is a glycoprotein with angiogenic and immune regulatory functions positively related to periodontitis and could probably be involved in the pro-inflammatory mechanisms observed in periodontal clinical tissue inflammation [ 103 ].…”

Section: The Most Promising Host Derived Biomarkers As Candidates mentioning

confidence: 99%

Predictive Periodontitis: The Most Promising Salivary Biomarkers for Early Diagnosis of Periodontitis

Cafiero

Spagnuolo

Marenzi

et al. 2021

JCM

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The primary cause of tooth loss in the industrialized world is periodontitis, a bacterial anaerobic infection whose pathogenesis is characterized by composite immune response. At present, the diagnose of periodontitis is made by a complete status check of the patient’s periodontal health; full-mouth plaque score, full-mouth bleeding score, probing depth, clinical attachment level, bleeding on probing, recessions, mobility, and migration are evaluated in order to provides a clear picture of the periodontal conditions of a single patient. Chair-side diagnostic tests based on whole saliva could be routinely used by periodontists for a very early diagnosis of periodontitis, monitoring, prognosis, and management of periodontal patients by biomarker detection, whose diagnostic validity is related to sensitivity and specificity. Recent paper reviews and meta-analyses have focused on five promising host derived biomarkers as candidate for early diagnosis of periodontitis: MMP-8 (Metalloproteinase-8), MIP-1α (Macrophage inflammatory protein-1 alpha), IL-1 β (Interleukin-1 beta), IL-6 (Interleukin-6), and HB (Hemoglobin), and their combinations. Chair-side Lab-on-a-chip (LOC) technology may soon become an important part of efforts to detect such biomarkers in saliva medium to improve worldwide periodontal health in developed nations as well as in underserved communities and poor countries. Their applications in preventive and predictive medicine is now fundamental, and is aimed at the early detection of risk factors or the presence or evolution of the disease, and in personalized medicine, which aims to identify tailor-made treatments for individual patients. The aim of the present paper is to be informative about host derived periodontal biomarkers and, in particular, we intend to report information about the most important immune response derived biomarkers and Hemoglobin as candidates to be routinely utilized in order to obtain a chair-side early diagnosis of periodontal disease.

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“…This was the first time that a biosensor was used to determine the concentration of NRP-1 in saliva. The concentration of sNRP-1 in gingival crevicular fluid (GCF) samples from periodontitis patients was determined [37]. This study discovered a good correlation between the results obtained from the SPRi and the ELISA measurements of NRP-1 levels.…”

Section: Discussionmentioning

confidence: 85%

New Biosensor for Determination of Neuropilin-1 with Detection by Surface Plasmon Resonance Imaging

Sankiewicz

Żelazowska-Rutkowska

Górska

et al. 2023

Sensors

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Neuropilin-1 is transmembrane protein with soluble isoforms. It plays a pivotal role in both physiological and pathological processes. NRP-1 is involved in the immune response, formation of neuronal circuits, angiogenesis, survival and migration of cells. The specific SPRI biosensor for the determination of neuropilin-1 was constructed using mouse monoclonal antibody that captures unbound NRP-1 form body fluids. The biosensor exhibits linearity of the analytical signal between 0.01 and 2.5 ng/mL, average precision value 4.7% and recovery between 97% and 104%. The detection limit is 0.011 ng/mL, and the limit of quantification is 0.038 ng/mL. The biosensor was validated by parallel determination of NRP-1 in serum and saliva samples using the ELISA test, with good agreement of the results.

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Soluble Neuropilin-1 in gingival crevicular fluid from periodontitis patients: An exploratory cross-sectional study

Cited by 8 publications

References 17 publications

Gingival crevicular fluid lipocalin‐2 and semaphorin3A in stage III periodontitis: Non‐surgical periodontal treatment effects

Gingival crevicular fluid lipocalin‐2 and semaphorin3A in stage III periodontitis: Non‐surgical periodontal treatment effects

Predictive Periodontitis: The Most Promising Salivary Biomarkers for Early Diagnosis of Periodontitis

New Biosensor for Determination of Neuropilin-1 with Detection by Surface Plasmon Resonance Imaging

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