Species Distribution, Antimicrobial Resistance, and Enterotoxigenicity of Non-aureus Staphylococci in Retail Chicken Meat

Lee, Soo In; Kim, Sun Do; Park, Ji Heon; Yang, Soo-Jin

doi:10.3390/antibiotics9110809

Cited by 16 publications

(19 citation statements)

References 59 publications

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“…No SCC mec type was identified in the case of the four mec A-positive CoNS. These findings are consistent with the results of previous studies in which non-typeable ccr genes were shown to be associated with the heterogeneity of SCC mec elements in methicillin-resistant CoNS strains [ 31 , 33 ].…”

Section: Discussionsupporting

confidence: 93%

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Garbacz

Wierzbowska

Kwapisz

et al. 2021

Journal of Oral Microbiology

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Background: The use of antibiotics in dentistry is associated with the emergence and spread of antibiotic-resistant microorganisms, including commensal staphylococci. Methods: A total of 367 oral samples were collected, from which staphylococci were isolated and identified by using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF). The antibiotic susceptibility of the isolates was determined and molecular characteristics for methicillin-resistant staphylococci was performed. Results: A total of 103 coagulase-negative staphylococci (CoNS), among them S. warneri, S. haemolyticus, S. saprophyticus, S. pasteuri, S. epidermidis, S. hominis, S. xylosus, S. equorum, S. kloosii, S. succinus, S. cohnii , and S. simulans , were confirmed by MALDI-TOF. Resistance to most tested antibiotics was statistically higher in CoNS than in S. aureus isolates ( P -value < 0.05). CoNS isolates showed high resistance to penicillin ( S. saprophyticus 88.9%), erythromycin ( S. haemolyticus 84.6%), fusidic acid ( S. saprophyticus 77.8%), co-trimoxazole ( S. epidermidis 71.4%), gentamicin ( S. warneri 63.8%), and tetracycline ( S. saprophyticus 55.6%). Multidrug resistance was largely observed, especially among S. haemolyticus and S. saprophyticus species. Methicillin-resistance in S. haemolyticus (38.5%), S. saprophyticus (22.2%) and S. aureus (13.5%) was associated with the presence of the mec A gene and SCC mec type IV or V. Conclusion: Coagulase-negative staphylococci, especially S. haemolyticus and S. saprophyticus , seem to be a reservoir of methicillin resistance and multidrug resistance in the oral cavity.

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Section: Discussionsupporting

confidence: 93%

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Garbacz

Wierzbowska

Kwapisz

et al. 2021

Journal of Oral Microbiology

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“…In addition to resistance to β-lactamates, resistance to tetracycline (86%) and erythromycin (78%) was also detected to a greater extent in the isolates we examined. Resistance to erythromycin and tetracycline in S. chromogenes was also detected by Lüthje et al and Lee et al [73,74]. However, in a study by Devries et al, out of 73 staphylococcal strains from different dairy farms, 70 strains of S. chromogemes sensitive to neomycin, gentamicin, erythromycin, enrofloxacin, and penicillin were detected [75].…”

Section: Discussionmentioning

confidence: 84%

Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep’s Milk and Cheese

et al. 2021

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Antimicrobial and multidrug resistance is detected in nonaureus staphylococci, including Staphylococcus chromogenes, which commonly causes intramammary infections. Recent clinical studies point to the presence of methicillin-resistant S. chromogenes. Therefore, this study aims to determine the prevalence of this species in samples of sheep‘s milk and cheeses made from them. Isolates were identified by polymerase chain reaction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF). A total of 208 staphylococcal isolates were identified. Of these, 18% were identified as S. chromogenes. The antimicrobial resistance of the identified isolates was determined using the agar dilution method against penicillin, ceftaroline, teicoplanin, gentamicin, erythromycin, tetracycline, and ofloxacin. The highest resistance was found to penicillin (95%), tetracycline (86%), and oxacillin (81%). The highest sensitivity was confirmed for gentamicin (55%). The study also confirmed the presence of methicillin resistant staphylococcal isolates (30%) based on the phenotypic manifestation of antimicrobial resistance and detection of the presence of the mecA gene. The study shows that the tested isolates (62%) were multidrug resistant. Resistance to two antibiotics was most often found (39%).

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“…Resistance to penicillin, ciprofloxacin, and tetracycline of analysed Staphylococcus succinus subsp. casei strains was similar to those observed in other CNS species (Lee et al ., 2020). They showed susceptibility to oxacillin and many other tested antibiotics, which is consistent with the characteristics of methicillin‐sensitive (MS) staphylococcal strains (Kwapisz et al ., 2020).…”

Section: Discussionmentioning

confidence: 99%

Novel staphylococci nucH taxonomical marker used in identification of human‐associated Staphylococcus succinus subsp. casei

Garbacz

Kotłowski

Kwapisz

2021

Environ Microbiol Rep

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Summary The aim of our study was to assess the sequencing of unique nucH gene fragment based on performed bioinformatics analysis as a novel diagnostic method for the identification of difficult to identify staphylococcal human pathogenic strains. Initially, PCR‐RFLP‐rrn analysis specific to the spacers between 16SrDNA and 23SrDNA followed by HhaI restriction analysis was performed. Further, sequencing of nucH and 16S rDNA genes fragments was carried out. Blast analysis from the NCBI showed 99% similarity of nucH gene fragment with reference genomic DNA for S. succinus with the accession no. CP018199. This result was also confirmed by MALDI‐TOF analysis. Sequencing analysis of 16S rDNA gene fragment allowed for 100% identification of two strains isolated from human samples as Staphylococus succinus subsp. casei. Sequencing of identified unique nucH gene fragment seems to be a promising diagnostic assay for the identification of Staphylococcus species. Based on our results, we can assume that probably other Staphylococcus species originated from different clinical samples could be identified using nucH gene sequencing method we developed. However, an extension of the genetic databases with a substantially bigger number of reference staphylococcal species for nucH gene is needed to make this method better than widely used standard 16S rDNA sequencing assay. To the best of our knowledge, it is the second published isolation of S. succinus subsp. casei from human clinical specimens. Moreover, possibility of decreasing the number of dimensions from multi‐PCR‐bands results using ribotyping analysis is also described.

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Species Distribution, Antimicrobial Resistance, and Enterotoxigenicity of Non-aureus Staphylococci in Retail Chicken Meat

Cited by 16 publications

References 59 publications

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep’s Milk and Cheese

Novel staphylococci nucH taxonomical marker used in identification of human‐associated Staphylococcus succinus subsp. casei

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