Spectrophotometric Determination of Paracetamol and Caffeine

Abstract

Two UV-visible methods are described, method A to determine paracetamol and caffeine in tablet formulations by spectroscopic derivative. Paracetamol was determined after solving it in sodium hydroxide by taking the first order derivative spectroscopy at 314 nm and caffeine was determined after solving it in bidistilled water by taking the second order derivative spectroscopy at 280 nm. Beers law is obeyed in the concentration ranges 0.78-31.07 µg/mL and 1.51-30.23 µg/mL for caffeine and paracetamol, respectively. Method B to determine paracetamol in tablet and oral formulations. This method is based on the reduction of Fe 3+ to Fe 2+ by paracetamol, the resulting Fe 2+ reacts with 1,10-phenanthroline to give a soluble orange-red complex in acetic acid medium. The maximal absorption is at the wavelength 510 nm. The complete reaction was achieved during heating time 25 min at 45 °C. Beers law is obeyed in the concentration range 1.21-7.56 µg/mL. The limit of detection, limit of quantification and linearity are calculated for both methods.

Spectrum subtraction method for simultaneous determination of acetaminophen and caffeine in panadol extra dosage forms

“…Statistical comparison between the proposed technique and the reference one 3 was done by One-way ANOVA method through utilizing PASW statistics 18 ® software program in which there was no significant difference between them (Table 3).…”

“…The literature showed that several methods were carried out for the analysis of APAP and CAF in their mixture form. APAP and CAF have been determined by spectrophotometric methods [3][4][5][6][7][8][9][10][11][12][13][14], chromatographic methods [15][16][17][18][19][20][21][22][23], voltammetric techniques [24][25][26], Near Infrared (NIR)-chemometric method [27] and Flow-through (FI) ultraviolet plus multioptosensing device technique [28].…”

Spectrophotometric Determination of Trace Zinc in Yeast by Using 2-(5-Bromo-2-pyridylazo)-5-diethylaminophenol

“…It was found that the HNO3-H2O2 (2:1) system has the best effect of dissolving sample. A hydrogen peroxide is extremely beneficial to digest organic compound 15 , 2.0 mL was added. Both of the pressure and temperature are most important parameters in the course of microwave digestion.…”

Simultaneous determination of paracetamol and caffeine by RP-HPLC in soft gelatin capsules

“…For the quantitative estimation of caffeine and paracetamol in various pharmaceutical dosage forms, several HPLC methods [16][17] have been reported. It has been reported that paracetamol and caffeine in formulations can be estimated using spectrophotometric [18] methods. However, a more precise, simple, and widely used HPLC method for determining caffeine and paracetamol simultaneously in soft gelatin capsules has not been published.…”