Spectrophotometric determination of propranolol in formulations via oxidative coupling with 3-methylbenzothiazoline-2-one hydrazone

El-Emam, Ali A.; Belal, F.; Moustafa, Mohamed A.; El‐Ashry, Saadia M.; El-Sherbiny, Dina T.; Hansen, Steen Honoré

doi:10.1016/s0014-827x(03)00199-x

Cited by 47 publications

(34 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…NBD derivatives are usually determined fluorimetrically, 29,30) however, photometric detection has also occasionally been used. 31,32) The aim of this study was to optimize the reaction between the alkaline hydrolytic products of the three studied drugs and NBD-Cl.…”

mentioning

confidence: 99%

Spectrofluorimetric and Spectrophotometric Stability-Indicating Methods for Determination of Some Oxicams Using 7-Chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl)

Taha

Salama

Fattah³

2006

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

mentioning

confidence: 99%

Spectrofluorimetric and Spectrophotometric Stability-Indicating Methods for Determination of Some Oxicams Using 7-Chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl)

Taha

Salama

Fattah³

2006

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

“…30) In recent reports, NBD-Cl was further used as a chromogenic reagent for the determination of some primary and secondary amines. [14][15][16][17][18][19][20][21][22][23][24] In the present study, DSL was found to react with NBD-Cl in borate buffer of pH 7.6 producing a yellow color with maximum absorbance at 485 nm; Method I. Figure 2a illustrates the absorption spectrum of the reaction product.…”

Section: Resultsmentioning

confidence: 87%

“…14) Moreover, the condensation product with NBD-Cl was found to be also a fluorescent derivative. NBD-Cl has been used as a derivatizing agent for many compounds either per se or in formulations and biological fluids such as lisinopril, 15) gliclazide, 16) tianeptine, 17) ranitidine and nizatidine, 18) atenolol, 19) fenetrol, 20) trimetazidine, 21) and recently befunolol, 22) some b-blockers 23) and paroxetine. 24) Sanger's reagent (DNFB) on the other hand has been applied as a chromophore reactant in the spectrophotometric determination of norfloxacin, 25) enalapril and lisinopril.…”

mentioning

confidence: 99%

Spectrophotometric, Spectrofluorometric and HPLC Determination of Desloratadine in Dosage Forms and Human Plasma

2007

View full text Add to dashboard Cite

Four sensitive, simple and specific methods were developed for the determination of desloratadine (DSL), a new antihistaminic drug in pharmaceutical preparations and biological fluids. Methods I and II are based on coupling DSL with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.6 where a yellow colored reaction product was obtained and measured spectrophotometrically at 485 nm (Method I). The same product could be measured spectrofluorometrically at 538 nm after excitation at 480 nm (Method II). Methods III and IV, on the other hand, involved derivatization of DSL with 2,4-dinitrofluorobenzene (DNFB) in borate buffer of pH 9.0 producing a yellow colored product that absorbs maximally at 375 nm (Method III). The same derivative was determined after separation adopting HPLC (Method IV). The separation was performed on a column packed with cyanopropyl bonded stationary phase equilibrated with a mobile phase composed of acetonitrile-water ( (Method IV). The four developed procedures were applied for the determination of DSL in tablets without any interference from the excipients. A low LOQ is a common requirement to support a clinical development program and when this is coupled with a simple procedure for sample preparation and feasible analytical tool, they would be the requirements of an optimized analytical method. Although the previously reported methods that were applied in pharmacokinetic studies of desloratadine possess the required low LOQ, they lack the simple sample preparation and method feasibility that were obtained in our spectrofluorometric method using NBD-Cl as a derivatizing agent where a low LOQ of 0.012 mg/ml was attained which allowed the analytical determination of DSL in spiked and real human plasma. ExperimentalMaterials and Reagents All chemicals were of analytical reagent grade. Desloratadine reference standard was kindly provided by ScheringPlough Corporation, U.S.A. Aerius tablets labeled to contain 5 mg DSL per tablet were purchased from commercial sources at a local pharmacy. Plasma samples were obtained from Mansoura University Hospital, Mansoura, Egypt, and were kept frozen until use after gentle thawing. Sep-Pak ® C18 solid phase extraction cartridges, 200 mg (SPE) were purchased from Waters Corporation (Milford, MA, U.S.A.). 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was purchased from Sigma (St. Louis, MO, U.S.A.). A stock solution containing 2 mg/ml was freshly prepared in methanol. 2,4-Dinitrofluorobenzene (DNFB) was also purchased from Sigma (St. Louis, MO, U.S.A.). A stock solution containing 0.25% (v/v) was freshly prepared in methanol. Borate buffer solutions (0.2 M) were prepared by mixing appropriate volumes of 0.2 M boric acid and 0.2 M NaOH and adjusting the pH to 7.6 and 9.0 using a pH meter.29) Formic acid 85%, acetonitrile and methanol; AR Grade, Aldrich (St. Louis, MO, U.S.A.).Apparatus A Shimadzu UV-Visible 1601 PC spectrophotometer was used for the spectrophotometric measurements (P/N 206-67001). The recording range was 0-1.0.The f...

show abstract

“…The analysis of different compounds or drugs either in pharmaceuticals or in biological samples was performed after derivatization with NBD-Cl followed by measuring the resulted product by means of spectrophotometry [46][47][48][49], Spectrofluorimetry [50][51][52], HPLC as in determination aliphatic amines [53].…”

Section: Resultsmentioning

confidence: 99%

“…The reaction products must be acidified to pH = 2 using HCl before measurement, in order to change NBD-OH to NBD-OCH3 [66,46]. It was found that maximum absorbance readings were obtained on using 0.5 and 1.0 mL 1 M HCl for propafenone HCl and diltiazem HCl, respectively.…”

Section: Effect Of Hcl Molarity and Volumementioning

confidence: 99%

Application of 4-chloro-7-nitrobenzofurazan for the analysis of propafenone and diltiazem hydrochlorides using kinetic spectrophotometric and spectrofluorimetric methods

et al. 2013

View full text Add to dashboard Cite

KEYWORDSSeveral simple, sensitive, accurate and inexpensive spectrophotometric and spectrofluorimetric methods were developed for the determination of propafenone HCl and diltiazem HCl using 4-chloro-7-nitrobenzofurazan (NBD-Cl) accompanied with kinetic study, either in pure form or in pharmaceutical preparations. In this work, the cited drugs react with (NBD-Cl) in presence of borate buffer of pH = 7.6 at a fixed time of 30 minutes on thermostated water bath at (75-80 °C). The absorbance was measured using spectrophotometric technique at 489 and 481 nm for propafenone HCl and diltiazem HCl, respectively, or by using spectrofluorimetric technique after dilution at the specific wavelength of excitation and emission.The calibration curves were linear in the range of 4-44, 16-96 µg/mL when using spectrophotometric method, and 0.4-3.6, 1.6-8.8 µg/mL when spectrofluorimetric method was applied for propafenone HCl and diltiazem HCl, respectively. The limit of quantitation and the limit of detection were also calculated. The methods were applied successfully to commercial dosage form and can be further applied for their determination on a large scale in quality control laboratories. The obtained results statistically agreed with those obtained by reference methods. The determination of the studied drugs by the fixed concentration and rate constant methods is feasible with the calibration equations obtained, but the fixed time method proves to be more applicable.Diltiazem HCl Propafenone HCl Spectroflourimetric Spectrophotometric Kinetic determination 4-Chloro-7-nitrobenzofurazan

show abstract

Spectrophotometric determination of propranolol in formulations via oxidative coupling with 3-methylbenzothiazoline-2-one hydrazone

Cited by 47 publications

References 26 publications

Spectrofluorimetric and Spectrophotometric Stability-Indicating Methods for Determination of Some Oxicams Using 7-Chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl)

Spectrofluorimetric and Spectrophotometric Stability-Indicating Methods for Determination of Some Oxicams Using 7-Chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl)

Spectrophotometric, Spectrofluorometric and HPLC Determination of Desloratadine in Dosage Forms and Human Plasma

Application of 4-chloro-7-nitrobenzofurazan for the analysis of propafenone and diltiazem hydrochlorides using kinetic spectrophotometric and spectrofluorimetric methods

Contact Info

Product

Resources

About