Stabilitas Fisik Dan Antioksidan Mikroemulsi Minyak Nilam Dengan Variasi Tween 80 Dan PEG 400

Abstract

Patchouli oil (Pogostemon cablin Benth.) has potential as an antioxidant and high volatility due to air and light, therefore it needs to be formulated in the form of a microemulsion. This research aims to determine the physical stability and antioxidant microemulsion of patchouli oil with different concentrations of Tween 80 and PEG 400. Microemulsions was prepared with patchouli oil content of 5% along with different concentration of Tween 80: PEG 400 were F1 (26%: 13%), F2 (13%: 26%) and for F3 (26%: 26%). The patchouli oil microemulsion was tested for physical stability by the cycling test method using a climatic chamber at a temperature of 40 oC ± 2oC and 4 oC ± 2oC for 6 cycles. Furthermore, the tests include organoleptic, homogeneity, viscosity, pH, and separation. The antioxidant test was carried out using the DPPH method, and the quantitative data on physical stability and antioxidants were analyzed by a t-dependent test. The stability test results showed that F2 and F3 had decreased viscosity after 6 storage cycles (p <0.05). The results of the homogeneity, pH and separation tests did not experience significant differences. The test results of patchouli oil microemulsion antioxidant activity showed no change in antioxidant activity during 6 storage cycles with the mean inhibition percent before and after the cycling test was 73,13 ± 0,38 %. Patchouli oil microemulsion with tween 80: PEG 400 26%: 13% showed the most stable physical quality and antioxidant properties.Keywords : Microemulsion, Patchouli Oil, Physical Stability, AntioxidantsMinyak nilam (Pogostemon cablin Benth.) berpotensi sebagai antioksidan dan memiliki laju volatilitas tinggi akibat udara dan cahaya sehingga perlu diformulasikan dalam bentuk mikroemulsi. Penelitian ini bertujuan untuk mengetahui stabilitas fisik dan antioksidan mikroemulsi minyak nilam dengan variasi konsentrasi tween 80 dan PEG 400. Mikroemulsi dibuat dengan kadar minyak nilam 5% dan variasi konsentrasi campuran tween 80:PEG 400 adalah untuk F1 (26%:13%) dan F2 (13%:26%) serta untuk F3 (26%:26%). Mikroemulsi minyak nilam diuji stabilitas fisik dengan metode cycling test menggunakan climatic chamber pada suhu 40 oC ± 2oC dan 4 oC ± 2oC selama 6 siklus. Pengujian meliputi organoleptis, homogenitas, viskositas, pH, dan uji pemisahan. Uji antioksidan dilakukan menggunakan metode DPPH. Data kuantitatif dari stabilitas fisik dan antioksidan dianalisis dengan uji t-dependent. Hasil uji stabilitas menunjukkan F2 dan F3 mengalami penurunan viskositas setelah 6 siklus penyimpanan (p < 0,05). Hasil uji homogenitas,pH dan uji pemisahan tidak mengalami perbedaan bermakna. Hasil uji aktivitas antioksidan mikroemulsi minyak nilam menunjukkan tidak terjadi perubahan aktivitas antioksidan selama 6 siklus penyimpanan dengan rerata persen inhibisi sebelum dan sesudah cycling test adalah 73,13 ± 0,38 %. Mikroemulsi minyak nilam F1 dengan tween 80: PEG 400 26%:13% menunjukkan Mutu fisik dan sifat antioksidan yang paling stabil.Kata kunci : Mikroemulsi, Minyak Nilam, Stabilitas Fisik, Antioksidan

Chemical qualitative analysis and spf value stability of nutmeg seed oil in microemulsions with tween 80 and PEG 400 as surfactants and cosurfactants

“…The data also showed that each formula also did not show any significant different of the SPF value (p > 0.05) The combination of tween 80 and PEG 400 can provide thermodynamically stable dosage result and is compatible with the active substances used. The previous study of the Tween 80 and PEG 400 combination in nutmeg oil showed stable microemulsion especially on physical and antioxidant activity within 6 cycles (Shabrina et al, 2021). The Tween 80 has long-chain fatty acid and are available to decrease the interfacial tension between the nutmeg oil and cosurfactants, which provides clear Microemulsion (Handa et al, 2021).…”

“…Nutmeg oil is added into the system until a clear and transparent microemulsion was formed. Stirring was carried out at a speed of 700 rpm for 30 minutes (Shabrina et al, 2021). NSM then stored in climatic chamber for 4 weeks at 30 °C ± 2 °C with RH 65 % ± 5 %.…”

“…NSM then stored in climatic chamber for 4 weeks at 30 °C ± 2 °C with RH 65 % ± 5 %. The microemulsion had been tested for globul size using Particle Size Anlyzer with range of 72 nm to 82 nm (Shabrina et al, 2021). The reference formula used according to (Shabrina et al, 2021) can be seen in Table 1.…”

“…Microemulsion of benzophenone-3 for sunscreen can improve UV-blocking activity (Badawi et al, 2014). The combination of Tween 80 and PEG 400 in the nutmeg oil microemulsion stable after six cycles of storage (Shabrina et al, 2021). Based on the above background, it is necessary to continue the research of nutmeg seed oil microemulsion using Tween 80 and PEG 400 to determine the chemical content and SPF value stability.…”

FORMULASI EMULGEL MINYAK BIJI PALA (Myristica fragans) SEBAGAI ANTIOKSIDAN

“…Minyak biji pala bersifat tidak larut dalam air sehingga dibutuhkan suatu bentuk sediaan yang mampu memfasilitasi pelarutan dan penetrasinya. Shabrina et al, (2020) mengembangkan formula sediaan mikroemulsi dengan kandungan minyak biji pala 6,4%. Dari hasil penelitian tersebut didapatkan mikroemulsi minyak biji pala memiliki aktivitas antioksidan kuat dengan persen inhibisi 64-68%.…”