Steady-State and Decay Characteristics of Protein Tryptophan Fluorescence from Bacteria

Dalterio, R. A.; Nelson, W. H.; Britt, Deborah E.; Sperry, J. F.; Psaras, Dimitrios; Tanguay, J. F.; Suib, S. L.

doi:10.1366/0003702864815439

Cited by 61 publications

(26 citation statements)

References 21 publications

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“…Shelly et al (1980a,b) and Dalterio et al (1986) proposed identification of bacteria by their fluorescence characteristics. Determann et al (1998) postulated that it may be possible to identify algal and bacterial tryptophan by emission wavelength, compared with that of tryptophan standard.…”

Section: Dissolved Organic Matter Fluorescencementioning

confidence: 99%

Fluorescence analysis of dissolved organic matter in natural, waste and polluted waters—a review

Hudson

Baker

Reynolds

2007

River Research & Apps

866

454

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Dissolved organic matter (DOM) in aquatic systems originates from a range of sources. Some is allochthonous, transported from the surrounding landscape to the water body, and is derived from and influenced by the geology, land use and hydrology of its origin. Some is created in situ through microbial activity, which may provide an independent source of organic matter, or a recycling mechanism for that which has been transported into the water body. The relative contribution of each source depends upon the location and environmental conditions within and without the water body. Human activity is also a source of DOM, much of which is believed to be labile, which can enter the aquatic system through direct point discharges, diffuse leaching and aerial dispersal. Fluorescence spectroscopy can provide an excellent tool to source DOM fractions, and to monitor and understand DOM transformations in aquatic systems, as much DOM has an intrinsic fluorescence. In particular, recent advances in optical technology, enabling rapid investigation of shorter wavelengths, have enabled more detailed characterization of organic material and its reactions in water. In this article, we review the use of fluorescence spectroscopic techniques to measure the intrinsic fluorescence of organic matter and the application of fluorescent DOM analysis in marine waters, freshwaters and wastewaters.

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Section: Dissolved Organic Matter Fluorescencementioning

confidence: 99%

Fluorescence analysis of dissolved organic matter in natural, waste and polluted waters—a review

Hudson

Baker

Reynolds

2007

River Research & Apps

866

454

View full text Add to dashboard Cite

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“…Autofluorescence excitation and emission (EEM) spectra can help in distinguishing among (64,65) different species of bacteria. Fluorescence spectra of bacteria (64)(65)(66)(67)(68)(69) are broad and tend to be similar for different species. From an instrument design viewpoint, it is much easier to measure the near ultraviolet (UV) and visible fluorescence spectra of individual airborne biological particles flowing through a laser beam than it is to rapidly measure the IR absorption spectrum of an individual particle.…”

Section: Fluorescence Of Biomaterials: Excitation-emission Spectramentioning

confidence: 99%

Fluorescence of Bacteria, Pollens, and Naturally Occurring Airborne Particles: Excitation/Emission Spectra

Hill¹,

Mayo²,

Chang³

2009

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Approved for public release; distribution unlimited. FOR OFFICIAL USE ONLYii REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number.

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“…Dalterio et al (1986) were able to establish fluorescent decay spectrums for a number of bacterial species, including E. coli. The techniques applied were significantly more complicated than sampling intrinsic fluorescence, requiring electronics and signal processing capabilities that are an order of magnitude more precise and expensive than those used in standard tryptophan fluorometers.…”

Section: Possible Mechanisms For Bacterial Tryptophan Fluorescencementioning

confidence: 99%

Assessing a Fluorescence Spectroscopy Method for In-Situ Microbial Drinking Water Quality

Sharpe¹

2000

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Waterborne disease is a significant contributor to the global burden of disease, in particular among high-risk populations in developing nations. State-of-the-art methods for the enumeration of microbial pathogens in drinking water sources have important limitations, including high initial cost, 24-48 hour delays in results, high staffing and facility requirements, and training requirements which all become especially problematic in the developing nation context.A number of alternative approaches to microbial water quality testing have been proposed, with the goal of decreasing the required testing time, decreasing overall costs, leveraging appropriate technology approaches, or improving sensitivity or specificity of the water quality testing method. One approach that may offer solutions to some of these limitations involves the deployment of sensor networks using fluorescent spectroscopy to detect intrinsic protein fluorescence in water samples as a proxy for microbial activity. In recent years, a number of researchers have found significant and meaningful correlations between indicator bacteria species and the protein fluorescence of drinking water samples. Additionally, advances in the semiconductor industry could be used to drive down the cost of such sensors. This technology may also be extensible to other water quality parameters, including dissolved organic matter or the presence of fluorescent pollutants.In this thesis, a literature review describes the fundamentals of fluorescence spectroscopy, historical and recent work regarding the fluorescence of the amino acid tryptophan and associated bacterial fluorescence, possible mechanisms for this ii association, and potential applications of this technology for drinking water quality monitoring and waste water process control. Extensibility of the technology is also discussed.Next, experimental methodology in reproduction of similar results is described.Samples were taken from seven (7) iv Acknowledgements

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Steady-State and Decay Characteristics of Protein Tryptophan Fluorescence from Bacteria

Cited by 61 publications

References 21 publications

Fluorescence analysis of dissolved organic matter in natural, waste and polluted waters—a review

Fluorescence analysis of dissolved organic matter in natural, waste and polluted waters—a review

Fluorescence of Bacteria, Pollens, and Naturally Occurring Airborne Particles: Excitation/Emission Spectra

Assessing a Fluorescence Spectroscopy Method for In-Situ Microbial Drinking Water Quality

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