Sterols of<i>Kalanchoe pinnata</i>: First report of the isolation of both C‐24 epimers of 24‐alkyl‐Δ<sup>25</sup>‐sterols from a higher plant

Akihisa, Toshihiro; Kokke, W. C. M. C.; Tamura, Toshitake; Matsumoto, Tarô

doi:10.1007/bf02536432

Cited by 42 publications

(13 citation statements)

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“…brasiliensis are mainly glycosylated flavonoids derived from aglycone patuletin [9], while for K . pinnata the constituents described are mainly steroids, bufadienolids, and glycosylated flavonoid derivatives of quercetin, kaempferol and luteolin aglycones [12, 16, 17, 18]. The main pharmacological studies of K .…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

et al. 2016

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The species Kalanchoe brasiliensis and Kalanchoe pinnata, both known popularly as “Saião,” are used interchangeably in traditional medicine for their antiophidic properties. Studies evaluating the anti-venom activity of these species are scarce. This study aims to characterize the chemical constituents and evaluate the inhibitory effects of hydroethanolic leaf extracts of K. brasiliensis and K. pinnata against local effects induced by Bothrops jararaca snake venom. Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography coupled with Diode Array Detection and Electrospray Mass Spectrometry (HPLC-DAD-MS/MS) were performed for characterization of chemical markers of the extracts from these species. For antiophidic activity evaluation, B. jararaca venom-induced paw edema and skin hemorrhage in mice were evaluated. In both models, hydroethanolic extracts (125–500 mg/kg) were administered intraperitoneally in different protocols. Inhibition of phospholipase enzymatic activity of B. jararaca was evaluated. The HPLC-DAD-MS/MS chromatographic profile of extracts showed some particularities in the chemical profile of the two species. K. brasileinsis exhibited major peaks that have UV spectra similar to flavonoid glycosides derived from patuletin and eupafolin, while K. pinnata showed UV spectra similar to flavonoids glycosides derived from quercetin and kaempferol. Both extracts significantly reduced the hemorrhagic activity of B. jararaca venom in pre-treatment protocol, reaching about 40% of inhibition, while only K. pinnata was active in post-treatment protocol (about 30% of inhibition). In the antiedematogenic activity, only K. pinnata was active, inhibiting about 66% and 30% in pre and post-treatment protocols, respectively. Both extracts inhibited phospholipase activity; however, K. pinnata was more active. In conclusion, the results indicate the potential antiophidic activity of Kalanchoe species against local effects induced by B. jararaca snake venom, suggesting their potential use as a new source of bioactive molecules against bothropic venom.

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Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

et al. 2016

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“…All the isolated compounds were identified by comparison of their MS and NMR spectra compared with data from the literature (McLean et al, 1987;Akihisa et al, 1991;Pouchert and Behnke, 1993;Faizi et al, 2001). Copies of the original spectra are obtainable from the author for correspondence.…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory compounds from leaves and root bark of Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg.

Mavar-Manga

Haddad

Pieters

et al. 2008

Journal of Ethnopharmacology

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“…In addition, Bufadienolides, bryophyllin-A, -B, -C and bersaldegenin-1, 3, 5-orthoacetate were isolated from the plant of Taiwan origin and was shown to possess hepato-protective activity and cytotoxicity [31,39-41,31]. Apart from this bryophyllol, bryophollone, bryophollenone, bryophynol, 2(9-decenyl) phenenthrene (I), 2(undecenyl) phenenthrene (II), 18α-oleanane, ψ-taraxasterol, α-and β-amyrins and their acetates, 24-epiclerosterol [24(R) stigmasta-5, 25-dien-3β-ol], 24(R) 5α-stigmasta-7, 25-dien-3β-ol, 5α-stigmast-24-en-3β-ol and 25-methyl-5α-ergost-24(28)-en-3β-ol were isolated from the plant leaves and other aerial parts [42,43]. Therefore, it is difficult to conclude from the present study that anti-HPV/anticancer activity observed in crude leaf extract or fraction F4 is displayed specifically by any single chemical entity.…”

Section: Discussionmentioning

confidence: 99%

Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells

Mahata

Maru

Shukla

et al. 2012

BMC Complement Altern Med

103

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Background Bryophyllum pinnata ( B. pinnata ) is a common medicinal plant used in traditional medicine of India and of other countries for curing various infections, bowel diseases, healing wounds and other ailments. However, its anticancer properties are poorly defined. In view of broad spectrum therapeutic potential of B. pinnata we designed a study to examine anti-cancer and anti-Human Papillomavirus (HPV) activities in its leaf extracts and tried to isolate its active principle. Methods A chloroform extract derived from a bulk of botanically well-characterized pulverized B . pinnata leaves was separated using column chromatography with step- gradient of petroleum ether and ethyl acetate. Fractions were characterized for phyto-chemical compounds by TLC, HPTLC and NMR and Biological activity of the fractions were examined by MTT-based cell viability assay, Electrophoretic Mobility Shift Assay, Northern blotting and assay of apoptosis related proteins by immunoblotting in human cervical cancer cells. Results Results showed presence of growth inhibitory activity in the crude leaf extracts with IC 50 at 552 μg/ml which resolved to fraction F4 (Petroleum Ether: Ethyl Acetate:: 50:50) and showed IC 50 at 91 μg/ml. Investigations of anti-viral activity of the extract and its fraction revealed a specific anti-HPV activity on cervical cancer cells as evidenced by downregulation of constitutively active AP1 specific DNA binding activity and suppression of oncogenic c-Fos and c-Jun expression which was accompanied by inhibition of HPV18 transcription. In addition to inhibiting growth, fraction F4 strongly induced apoptosis as evidenced by an increased expression of the pro-apoptotic protein Bax, suppression of the anti-apoptotic molecules Bcl-2, and activation of caspase-3 and cleavage of PARP-1. Phytochemical analysis of fraction F4 by HPTLC and NMR indicated presence of activity that resembled Bryophyllin A. Conclusions Our study therefore demonstrates presence of anticancer and anti-HPV an activity in B . pinnata leaves that can be further exploited as a potential anticancer, anti-HPV therapeutic for treatment of HPV infection and cervical cancer.

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Sterols ofKalanchoe pinnata: First report of the isolation of both C‐24 epimers of 24‐alkyl‐Δ²⁵‐sterols from a higher plant

Cited by 42 publications

References 42 publications

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

Anti-inflammatory compounds from leaves and root bark of Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg.

Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells

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Sterols ofKalanchoe pinnata: First report of the isolation of both C‐24 epimers of 24‐alkyl‐Δ25‐sterols from a higher plant

Cited by 42 publications

References 42 publications

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom

Anti-inflammatory compounds from leaves and root bark of Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg.

Anticancer property of Bryophyllum pinnata (Lam.) Oken. leaf on human cervical cancer cells

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Sterols ofKalanchoe pinnata: First report of the isolation of both C‐24 epimers of 24‐alkyl‐Δ²⁵‐sterols from a higher plant