Stimulation of embryogenesis in Citrus tissue culture by galactose

Kochba, J.; Spiegel-Roy, P.; Saad, S.; Neumann, H.

doi:10.1007/bf00368574

Cited by 42 publications

(15 citation statements)

References 7 publications

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“…The autonomous growth of callus indicates an auxin:cytokinin balance that favors habituation. In citrus, somatic embryogenesis from callus has been obtained by different treatments, including reduction of sucrose in the culture medium (Kochba and Button, 1974); a change in the carbohydrate source to galactose instead of sucrose (Kochba et al, 1978b(Kochba et al, , 1982Hidaka and Omura, 1989;Cabasson et al, 1995;Kunitake and Mii, 1995), or to carbohydrates that produce galactose in their metabolism, such as lactose or raffinose (Kochba et al, 1978b;447 CITRUS SOMATIC EMBRYOGENESIS Ling and Iwamasa, 1997); or by using auxin (Kochba et al, 1978a) or gibberellic acid synthesis inhibitors (Spiegel-Roy and Saad, 1986).…”

Section: Introductionmentioning

confidence: 98%

“…Citrus plant regeneration can be obtained from callus cultures by somatic embryogenesis (Kochba et al, 1978b;Cabasson et al, 1995;Ling and Iwamasa, 1997). Although somatic embryogenesis is considered widespread in citrus polyembryonic varieties (Ranga Swamy, 1958;Kochba and Spiegel-Roy, 1977;Kunitake and Mii, 1995), several factors influence this process.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Somatic embryogenesis in Citrus SPP.: Carbohydrate stimulation and histodifferentiation

Tomaz¹,

Mendes²,

Filho³

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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Somatic embryogenesis from nucellus-derived callus cultures of five cultivars, including three (Caipira, Seleta Vermelha, and Valencia) of sweet oranges (C. sinensis L. Osbeck), Rangpur lime (C. limonia L. Osbeck), and Cleopatra mandarin (C. reticulata Blanco) (lines I and II), were studied. Callus lines maintained on MT medium supplemented with 50 g l 21 sucrose were transferred to MT medium supplemented with different carbohydrate sources: galactose, glucose, lactose, maltose, or sucrose at 18, 37, 75, 110, or 150 mM, or glycerol at 6, 12, 24, 36, or 50 mM. Globular embryos were observed after approximately 4 wk, in several treatments. Cultures of Valencia and Caipira sweet oranges and Cleopatra mandarin (line I) showed high numbers of embryos on medium containing galactose, lactose, and maltose. Histological studies showed somatic embryos in all developmental stages with a normal histodifferentiation pattern. The other two cultivars (Rangpur lime and Cleopatra mandarin, line II) formed very few embryos, which did not develop further following the globular stage. Some of the abnormalities observed were lack or dedifferentiation of protoderm and absence of apical meristems and procambial strands. Embryos that followed the normal sequence of development were easily converted into plants. Non-embryogenic cultures continued as proliferating callus cultures, eventually forming a few embryos which did not convert into plants. Statistical analyses of the callus response to carbohydrate treatments was done using an overdispersion Poisson model.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in Citrus SPP.: Carbohydrate stimulation and histodifferentiation

Tomaz¹,

Mendes²,

Filho³

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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“…We have observed, in fusion experiments involving other parental combinations, that many embryos developed in the same EME basal medium when sucrose was substituted by maltose (25 g/l) (Perez et al, 1998). Several studies have demonstrated the positive influence of other carbon sources, including lactose (Ling et al, 1990;Kunitake et al, 1991), galactose (Kochba et al, 1978(Kochba et al, , 1982 and glycerol (Vu et al, 1993), on the somatic embryogenesis of citrus plants.…”

Section: Resultsmentioning

confidence: 93%

Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

et al. 2000

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Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.

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“…Villafranca lemon and Shamouti oranges. Galactose, lactose and raffinose stimulated embryogenesis considerably in Citrus (Kochba et al, 1978b(Kochba et al, , 1982. The addition of lactose (5%) in the culture medium helped embryoid formation in long term callus cultures of C. sinensis cv.…”

Section: Culture Initiation Developmentmentioning

confidence: 98%