Stress Alterations in Growth Parameters, Pigment Content and Photosynthetic Functions of in vitro Cultured Plants

Kissimon, J.; Tantos, Á.; Mészáros, Annamária; Jámbor-Benczúr, E.; Horváth, Gábor

doi:10.1515/znc-1999-9-1033

Cited by 15 publications

(7 citation statements)

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“…Triacontanol, a long 30-carbon primary alcohol naturally occurring plant growth promoter is known to increase the growth and yield of some plants both in vivo and in vitro (Ries 1985;Kissimon et al 1999;Tantos et al 1999). We found that TRIA added to the liquid MS medium supplemented with IAA and BAP stimulated sage shoot multiplication at all concentrations tested (5, 10 or 20 µg l -1 ).…”

Section: Resultsmentioning

confidence: 75%

Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; effect of triacontanol

Grzegorczyk

Wysokińska

2011

Acta Soc Bot Pol

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Liquid shoot culture of Salvia officinalis L. in MS medium containing IAA (0.1 mg l -1 ) and BAP (0.45 mg l -1 ) was developed and evaluated in relation to shoot multiplication and antioxidant compound (carnosic acid, carnosol and rosmarinic acid) accumulation. In the liquid medium, on average, 3 new shoots per explant (shoot tip) were obtained within 3 weeks. The shoots produced 8.2±0.02 mg of diterpenoids and 31.2±0.29 mg of rosmarinic acid per gram of dry weight. Shoot proliferation and diterpenoid content increased when triacontanol (5, 10 or 20 µg l -1 ) was added to the liquid medium. In optimum conditions (at 20 µg l -1 TRIA) almost 7 shoots were formed per explant after 3 weeks. An increase in diterpenoid production (expressed as the sum of carnosol and carnosic acid) ranged from 30% to 50% and dependended on triacontanol concentration tested. The level of diterpenoids in triacontanol-treated shoots was similar to the content of compounds in commercial herbal product (dried leaves of S. officinalis) (10-12 mg g -1 dry wt). Triacontanol did not increase rosmarinic acid production, but the content of the phenolic as compound in shoots grown in liquid culture (31 mg g -1 dry wt) was even 24 times higher compared to samples of dried leaves of S. officinalis plants. We also demonstrated that the highest amounts of CA, Car and RA were accumulated in young, top parts of sage shoots. This observation could be useful for improving the selection of material for the extraction of natural antioxidants from S. officinalis.

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Section: Resultsmentioning

confidence: 75%

Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; effect of triacontanol

Grzegorczyk

Wysokińska

2011

Acta Soc Bot Pol

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“…The plant growth stimulating properties of TRIA (such as, increase in dry weight, leaf area and level of reducing sugars, amino acids, soluble proteins and total nitrogen) have been demonstrated by many researchers in various plant species (Ries 1985). Some authors have observed the role of TRIA in micropropagation of ornamental and other plants (Kissimon et al 1999;Reddy et al 2002). However, very few information is available on the effect of this natural growth regulator on biosynthesis of secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.

et al. 2011

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This report describes the effect of triacontanol on shoot multiplication and production of antioxidant compounds (carnosic acid, carnosol and rosmarinic acid) in S. officinalis cultures grown on MS basal medium (agar solidified medium supplemented with 0.1 mg l^-1 IAA, 0.45 mg l^-1 BAP). It was found that shoot proliferation significantly increased when triacontanol at concentrations of 5, 10 or 20 µg l^-1 was added to the medium. HPLC analysis of acetone and methanolic extracts of sage shoots showed that the production of diterpenoids, carnosic acid/carnosol ratio, as well as, contents of rosmarinic acid were also affected by the treatment with triacontanol. The highest stimulation effect of triacontanol was observed on the production of carnosol, where the treatment with 20 µg l l^-1 increased the content of this diterpenoid 4.5-fold compared to that in the control (sage shoots growing on MS basal medium, only)

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“…Chlorophyll a+b were measured according to Inskeep & Bloom (1985), and carotenoids were estimated by Kissimon (1999). The transpiration rate was measured as mentioned by Udayakumar et al (1998).…”

Section: Chemical Analysismentioning

confidence: 99%

The Influence of Spirulina platensis on Physiological Characterization and Mitigation of DNA Damage in Salt-stressed Phaseolus vulgaris L. Plants

Taha

Moussa²,

Dessoky³

2023

Egypt. J. Bot.

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S ALINITY severely reduced crop productivity. In this work, the effectiveness of Spirulina platensis (100mg/L) as a foliar growth stimulator was tested for its ability to reduce the harmful effect of salinity on Phaseolus vulgaris L. plants. The experiment was divided into four groups as follows: G 1 (control), G 2 (200mM NaCl), G 3 (100mg/L of Spirulina platensis), and G 4 (200mM NaCl + 100mg/L of Spirulina platensis). The results declared that shoot weight, plant height, leaves number, during the vegetative growth stage, pods number/plant, seed number/ pod, pods weight during fruiting stage, as well as content of carotenoids, chlorophyll a+b, 100seed weights, photosynthetic activity (assimilation of 14 CO 2 ), and total protein content reduced considerably in salt-stressed Phaseolus vulgaris. When compared to salt-stressed plants, the above metrics were enhanced by G 4 . In comparison to the control, the presence of salinity increased the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), peroxidase activity (POD), malondialdehyde (MDA), free proline, transpiration rate, and total phenol. Additionally, compared to plants under salt stress, G 4 application (200mM NaCl + 100mg/L of Spirulina platensis) decreased the aforementioned metrics. Treatments of Phaseolus vulgaris with 200mM NaCl + 100mg/L of Spirulina platensis considerably increased the macronutrient content and decreased the Na + and Cl − levels as compared to G 2 . Obtained results proved that foliar applications of S. platensis at 100 mg/l have a high potential for improving growth, photosynthetic capacity, yield production, decreased ROS-induced oxidative damage, and reducing DNA damage in salt-stressed Phaseolus vulgaris.

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Stress Alterations in Growth Parameters, Pigment Content and Photosynthetic Functions of in vitro Cultured Plants

Cited by 15 publications

References 0 publications

Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; effect of triacontanol

Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; effect of triacontanol

The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.

The Influence of Spirulina platensis on Physiological Characterization and Mitigation of DNA Damage in Salt-stressed Phaseolus vulgaris L. Plants

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