Structural Changes in the Cell Wall of<i>Schizosaccharomyces pombe</i>During Cell Division

Streiblová, Eva; Málek, I; Beran, K.

doi:10.1128/jb.91.1.428-435.1966

Cited by 41 publications

(12 citation statements)

References 25 publications

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“…Besides that, as the cell wall extension proceeds, the new mannan molecules are incorporated also over the whole surface of the cell wall in accordance with the rate of growth at the given site. The observed mode of growth in S. cerevisiae differs from that observed in hyphae (1, 6) or in the fission yeast, Schizosaccharomyces pombe (7,14,18), in that, besides the tip growth, the non-polarized or spherical extension also plays an important role. The ellipsoidal shape of the S. cerevisiae cells is thus acquired by a combination of these two modes of wall growth.…”

Section: Resultscontrasting

confidence: 57%

Autoradiographic Study of Mannan Incorporation into the Growing Cell Walls of Saccharomyces cerevisiae

et al. 1974

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The incorporation of mannan labeled by D-[1-3H]mannose into the growing cell walls of Saccharomyces cerevisiae has been followed by means of high-resolution autoradiography. The results show that, in the very first stage of budding, when the shape of the bud is nearly spherical, the new mannan is incorporated uniformly over the whole surface of the emerging bud. Later on, as the bud growth proceeds, the polarized tip growth predominates. In the maturation phase of bud growth, when the size of the bud is about two-thirds that of the mother cell, again a considerable incorporation of the labeled substrate over the whole bud surface was observed. The cell wall of the mother cell remained largely unlabeled. These facts indicate that the ellipsoidal shape of the cell walls in S. cerevisiae is the result of the combination of polarized tip growth and nonpolarized spherical extension.

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Section: Resultscontrasting

confidence: 57%

Autoradiographic Study of Mannan Incorporation into the Growing Cell Walls of Saccharomyces cerevisiae

et al. 1974

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“…D, Erosion of outer surface of old cell wall and first appearance of mtd at outside (now fuscannel). [24], Fig. 3C, D), somewhat more transparent than the innermost parts of the old cell wall but still less transparent than the AR.…”

Section: Resultsmentioning

confidence: 94%

“…D, Establishment of dense fillets at base of AR. E, Initiation of scar plug layer (SP [24]); fusion of dense body (DB) with dark layer (DL, arrow). F, Migration of dense fillet into wall to become materiel triangulaire dense (MTD [19]); closure of pore.…”

Section: Resultsmentioning

confidence: 99%

“…Nuclear events at mitosis are now well described (14), but the analysis of cytokinesis (fission in this case) is incomplete. For example, an interesting and heuristically useful study (24) emphasized the final product, whereas others (19) studied mostly the early stages of formation of the cell plate.…”

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confidence: 99%

“…Our intention is to blend some older observations of fission (12,15,16,19,24) with new data, to attempt a more complete description. For instance, an "electron-dense ring" (24), hereafter called "fuscannel" (fuscus, L., being dark; anellus, L., small ring, diminutive of anus) by us, external to the cell wall at the division scar (15,16), will be seen to derive from "materiel triangulaire dense" (19) found within ' Issued as National Research Council of Canada publication no.…”

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confidence: 99%

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Cell Division in Yeasts: Movement of Organelles Associated with Cell Plate Growth of Schizosaccharomyces pombe

1973

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Electron microscopy of dividing fission yeast cells shows establishment of an annular rudiment (AR) of electron-transparent material under the old cell wall as the first sign of elaboration of the cell plate. The AR grows centripetally, finally closing at the mid-point of the cell. During the inward growth of the AR it is thickened by addition of denser material which becomes the scar plug after fission; the electron-transparent material is lost at fission. Lying always between the cytoplasmic membrane and the cell wall is a dark layer of variable thickness. This layer becomes markedly thickened into a fillet at the base of the centripetally growing cell plate. The fission process begins after the cell plate is completely elaborated. One striking feature of fission is the migration of dense material from the fillet at the base of the cell plate outwardly through the matrix of the cell wall to its final resting place as a dark ring, a "fuscannel," adjacent to the fission scar. The inclusion of Golgi bodies in many sections suggests their involvement in cell plate elaboration, presumably through production of the dense bodies which are seen to fuse with the dark layer proximal to the growing cell plate.

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Cell wall growth during the cell cycle of Schizosaccharomyces pombe

Streiblová

Wolf

1972

J of Basic Microbiology

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Cell wall growth of an asynchronous culture of Schizosaccharomyces pombe was studied by fluorescence microscopy and time‐lapse cinematography. The cell wall growth of individual cells is a time‐ordered process which can be divided into 4 stages: post‐division stage, stage of growth initiation, stage of extension growth, and constant length stage. Different aspects of these stages are discussed. During the exponential phase of growth the population has a rather stable structure from the point of view of division scar numbers. The significance of the division scars for the study of cell wall growth and multiplication is discussed.

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Structural Changes in the Cell Wall ofSchizosaccharomyces pombeDuring Cell Division

Cited by 41 publications

References 25 publications

Autoradiographic Study of Mannan Incorporation into the Growing Cell Walls of Saccharomyces cerevisiae

Autoradiographic Study of Mannan Incorporation into the Growing Cell Walls of Saccharomyces cerevisiae

Cell Division in Yeasts: Movement of Organelles Associated with Cell Plate Growth of Schizosaccharomyces pombe

Cell wall growth during the cell cycle of Schizosaccharomyces pombe

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