Structure and function of the nontranscribed spacer regions of yeast rDNA

Skryabin, K. G.; Eldarov, M. A.; Larionov, Vladimir; Bayev, A.A.; Klootwijk, J.; Regt, V.C.H.F. de; Veldman, Geertruida M.; Planta, Rudi J.; Georgiev, O.I.; Hadjiolov, A.A.

doi:10.1093/nar/12.6.2955

Cited by 133 publications

(86 citation statements)

References 48 publications

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“…The S. cerevisiae rDNA enhancer sequence is unique in the rDNA repeat, bearing little or no homology to the downstream promoter region (45). In contrast, the Xenopus spp.…”

Section: Resultsmentioning

confidence: 99%

An RNA polymerase I enhancer in Saccharomyces cerevisiae.

Elion¹,

Warner²

1986

Mol. Cell. Biol.

160

133

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By the use of an artificial gene coding for rRNA (rDNA gene), we found that transcription of the major precursor rRNA in Saccharomyces cerevisiae cells is stimulated 15-fold by a positive control element located 2 kilobases upstream of the transcription initiation site. Analysis of in vitro runon transcripts suggests that this promoter element increases the frequency of initiation by RNA polymerase I molecules. A 190-base-pair fragment encompassing the promoter element can stimulate transcription on a centromere plasmid in either orientation, upstream or downstream of the transcription initiation site, suggesting that it is an enhancer element. Integration of artificial rDNA genes into a nonribosomal locus in the genome demonstrates that the rDNA enhancer functions either 5' or 3' to an rRNA transcription unit, suggesting it may operate in both directions within the rDNA tandem array. This is the first observation in S. cerevisiae of the stimulation of transcription by an element placed downstream. Finally, enhancer activity is dependent upon sequences that lie at both boundaries of the 190-base-pair fragment. In particular, a 5-base-pair deletion at the extreme 3' boundary of the 190-base-pair fragment greatly reduces the activation of transcription and implicates a set of inverted repeats.

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“…The S. cerevisiae rDNA enhancer sequence is unique in the rDNA repeat, bearing little or no homology to the downstream promoter region (45). In contrast, the Xenopus spp.…”

Section: Resultsmentioning

confidence: 99%

An RNA polymerase I enhancer in Saccharomyces cerevisiae.

Elion¹,

Warner²

1986

Mol. Cell. Biol.

160

133

View full text Add to dashboard Cite

show abstract

“…Many of these origins (or ARSs) were identified fortuitously because of their proximity to studied genes (e.g. Feldman et al 1984;Kearsey 1984;Skryabin et al 1984), or through random, plasmid-based assays (e.g. Chan & Tye 1980).…”

Section: Discussionmentioning

confidence: 99%

Replication profile of Saccharomyces cerevisiae chromosome VI

1997

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“…Chromosome XII contains ∼100-200 tandem copies of the 9.1-kb rDNA repeat (Skryabin et al 1984) as well as ∼1 Mb of the non-rDNA sequence. Each rDNA repeat has six Rrm3p-dependent sites; resolution of forks converged at the replication fork barrier (RFB) is particularly delayed in rrm3 cells (Ivessa et al 2000).…”

Section: The Rrm3p Helicase Affects Replication Of Every Yeast Chromomentioning

confidence: 99%

TheS. cerevisiaeRrm3p DNA helicase moves with the replication fork and affects replication of all yeast chromosomes

Azvolinsky¹,

Dunaway²,

Torres³

et al. 2006

Genes Dev.

169

172

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The Saccharomyces cerevisiae DNA helicase Rrm3p is needed for normal fork progression through >1000 discrete sites scattered throughout the genome. Here we show that replication of all yeast chromosomes was markedly delayed in rrm3 cells. Delayed replication was seen even in a region that lacks any predicted Rrm3p-dependent sites. Based on the pattern of replication intermediates in two-dimensional gels, the rate of fork movement in rrm3 cells appeared similar to wild-type except at known Rrm3p-dependent sites. These data suggest that although Rrm3p has a global role in DNA replication, its activity is needed only or primarily at specific, difficult-to-replicate sites. By the criterion of chromatin immunoprecipitation, Rrm3p was associated with both Rrm3p-dependent and -independent sites, and moved with the replication fork through both. In addition, Rrm3p interacted with Pol2p, the catalytic subunit of DNA polymerase , in vivo. Thus, rather than being recruited to its sites of action when replication forks stall at these sites, Rrm3p is likely a component of the replication fork apparatus.[Keywords: Rrm3p; Mrc1p; DNA replication; helicase; yeast; chromatin] Supplemental material is available at http://www.genesdev.org.

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Structure and function of the nontranscribed spacer regions of yeast rDNA

Cited by 133 publications

References 48 publications

An RNA polymerase I enhancer in Saccharomyces cerevisiae.

An RNA polymerase I enhancer in Saccharomyces cerevisiae.

Replication profile of Saccharomyces cerevisiae chromosome VI

TheS. cerevisiaeRrm3p DNA helicase moves with the replication fork and affects replication of all yeast chromosomes

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