Studies on the Biochemistry of Tetrahymena. Vii. Riboflavin, Pantothen, Biotin, Niacin and Pyridoxine in the Growth of T. Geleii W

Tissue Culture Nutrition

“…t u r e s is summarized in table 2. From these been shown by the studies of Kidder and Dewey studies (table 2), it seems that the cells so far (88,89) with Tetrahymena, and those of Morgan t and ortn (14,116 wih cickheat.fbro tested have a restricted and highly selective and Morton (114, 116) with chick heart fibro- Precise information on the pathways of carboi hydrate utilization in tissue cultures is extremely be regarded as largely preliminary steps inm the limited. Wilson et al (192) and Willmer (190) ultimate elucidation of precise information.…”

“…of metabolism, a suggestion that is in agreement The amino acid requirements recently deterwith the finding by Westfall et al (182) that minedfor chick embryonic heart fibroblasts (118), HeLa cells cultivated in serum and embryo the L strain (25, 32), HeLa cell (26,32), Walker extract cause an appreciable accumulation of 256 carcinosarcoma (106), and rabbit fibroblasts a-keto acids in the culture medium. Glutamic 67 poses, the amino acid requirements of Tetra- (88,89) have established the complexity of the hymena geleii (88) and of the adult human (148) amino acid interrelationships and have distinare included. In general, there is a remarkable guished between the absolutely essential amino similarity in the pattern of essential and non-acids and those which are stimulating because essential amino acids for the various types of of a slow rate of synthesis by the organism.…”