Studies on the Nutritional Requirements of Streptomyces griseus for the Formation of Streptomycin

Abstract

In view of the marked activity of streptomycin in vitro against a number of species of organisms relatively resistant to penicillin, such as Escherichia coli

Improvement in Streptomycin-Producing Strains of Streptomyces Griseus by Ultraviolet and X-Ray Energy

“…Since 1945, when the first encouraging reports on streptomycin appeared, improvements in its production by fermentation have been primarily medium improvements. Rake and Donovick (1946) have shown the value of a soybean meal medium. A yeast extract and salts medium has been developed by LePage and Campbell (1946) for greater ease of extraction.…”

UTILIZATION OF CARBOHYDRATES BY STRAINS OF STREPTOMYCES GRISEUS

“…Two basal media were used in these experiments. The soybean meal medium, based on that described by Rake and Donovick (1946), contained 30 g of soybean meal, 20 g of carbohydrate under study, and 1 L of distilled water. In this medium the soluble carbohydrates contributed by the soybean meal were negligible, amounting to less than 1 g per L (Street and Bailey, 1915).…”

Observations on the Multiplication of Phages Affecting Streptomyces Griseus

“…Wheat grain growth, 5 to 7 days old, of the cultures to be tested was used to inoculate the germination flasks (6 to 8 grains per flask). The germination medium, patterned after the one described by Rake and Donovick (1946), consisted of soybean meal, 1.5 g; NaCl, 0.5 g; cerelose, 2.16 g; distilled H20, 100 ml; final pH 5.7 to 6.0. After 40 hours' growth on a reciprocating shaker at 25 C, 10-ml transfers were made to fermentation flasks whose formula was soybean meal, 1.5 g; NaCl, 0.1 g; cerelose, 2.16 g; distilled H20, 100 ml; final pH 6.7 to 7.0.…”