Surface coating of hydroxyapatite on silk nanofiber through biomineralization using ten times concentrated simulated body fluid and the evaluation for bone regeneration

Lee, Minjung; Park, Jong Bo; Kim, Hyung Hwan; Ki, Chang Seok; Park, Sook Young; Kim, Hyun Jeong; Park, Young Hwan

doi:10.1007/s13233-014-2114-x

Cited by 31 publications

(12 citation statements)

References 27 publications

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“…Recently, silk‐based composite nanofibers incorporated in HA nanoparticles and bone morphogenic protein 2 (BMP‐2) have been fabricated as to enhance bone formation . As expected, the addition of HA and BMP‐2 in electrospun scaffolds caused higher rate of calcium deposition than that on pristine samples . Human mesenchymal stem cells were cultured on eri silk fibroin (ESF) (diameter = 800 m) and ESF/HA (diameter = 1000–1200 nm) scaffolds; then, cytocompatibity, blood compatibility, cells attachment, and growth were studied .…”

Section: Most Electrospun Polymers Used For Bone Regenerationmentioning

confidence: 93%

Electrospun biodegradable nanofibers scaffolds for bone tissue engineering

Khajavi

Abbasipour

Bahador

2015

J of Applied Polymer Sci

150

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Many polymeric materials have been developed and introduced for bone regeneration. Especially, their nanofibrous forms are mostly applied for artificial extracellular matrices. Polymeric materials in their nanofibrous form show some potent properties such as high surface-to-volume ratio, tunable porosity, and ease of surface functionalization. Benefiting from the properties of their main polymer and additives, they can provide new opportunities for cell seeding, proliferation, and new 3D-tissue formation. This article focuses on most cited polymeric nanofibrous scaffolds fabricated by electrospinning and recent achievements. They were divided into two main categories: natural (collagen, silk, keratin, gelatin, chitosan, and alginate) and synthetic (e.g., polycaprolactone, polylactic acid, and polyglycolic acid) polymers. The role of several additives like hydroxyapatite, bone morphogenetic proteins (BMPs), tricalcium phosphate, and collagen type I in improving the adhesion, differentiation, and tissue formation of stem cells were discussed. Finally, the osteogenic capacity and ability of nanofibrous scaffolds to support the growth of clinically relevant bone tissue were briefly studied.

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Section: Most Electrospun Polymers Used For Bone Regenerationmentioning

confidence: 93%

Electrospun biodegradable nanofibers scaffolds for bone tissue engineering

Khajavi

Abbasipour

Bahador

2015

J of Applied Polymer Sci

150

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“…Silk fibroin, another fibrous protein that closely mimics collagen type I of bone has gained popularity in tissue engineering due to interesting intrinsic properties such as resorbability [5], toughness [6], minimal immunogenicity [7,8], cytocompatibility with abundant polar, hydrophilic groups [9], strong chemical bonding with HA [10][11][12][13] and versatility in processing depending upon the target application.…”

Section: Introductionmentioning

confidence: 99%

“…14 Silk fibroin template fabricated in the form of nano fibres [12], films [15], porous 3D matrices [16] and composites [17] exposed to supersaturated ions, SBF [4,10] or fetal bovine serum [11], have been tested before for HA deposition. Silk fibroin plays a crucial role in regulating the synthesis and growth of HA nanocrystals [18], as HA combines with fibroin through chemical interactions which have been identified by FTIR analyses whereby the strong chemical bonding between the two caused peak shifts in the amide bonds of fibroin, commonly referred to as the 'blue shift' [2].…”

Section: Introductionmentioning

confidence: 99%

“…Also, the reason as to why mineralisation could not be observed on methanol treated pure fibroin films (without calcium chloride) which contained at least some amount of â-sheet content remained unexplained. Nevertheless, most studies have reported an additional requirement of either pre-exposure of fibroin or its association with calcium-and phosphate-based solutions to promote nucleation and growth of HA nanocrystals [12,13,16,22]. Whether it is the processing parameters during dissolution and reconstitution that disrupt the native structure of the fibroin chain; firstly by dissociating the specific amino acid chains and secondly by hampering their tendency to re-assemble the necessary â-sheet conformation, or if it is the inherent inert nature of fibroin chain that modulates the extent of mineralisation is still under wraps.…”

Section: Introductionmentioning

confidence: 99%

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Elucidation of differential mineralisation on native and regenerated silk matrices

Midha¹,

Tripathi²,

Geng

et al. 2016

Materials Science and Engineering: C

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Bone mineralisation is a well-orchestrated procedure triggered by a protein-based template inducing the nucleation of hydroxyapatite (HA) nanocrystals on the matrix. In an attempt to fabricate superior nanocomposites from silk fibroin, textile braided structures made of natively spun fibres of Bombyx mori silkworm were compared against regenerated fibroin (lyophilised and films) underpinning the influence of intrinsic properties of fibroin matrices on HA nucleation. We found that native braids could bind Ca 2+ ions through electrostatic attraction, which initiated the nucleation and deposition of HA, as evidenced by discrete shift in amide peaks via ATR-FTIR. This phenomenon also suggests the involvement of amide linkages in promoting HA nucleation on fibroin. Moreover, CaCl 2 -SBF immersion of native braids resulted in preferential growth of HA along the c-axis, forming needle-like nanocrystals and possessing Ca/P ratio comparable to commercial HA. Though regenerated lyophilised matrix also witnessed prominent peak shift in amide linkages, HA growth was restricted to (211) plane only, albeit at a significantly lower intensity than braids. Regenerated films, on the other hand, provided no crystallographic evidence of HA deposition within 7 days of SBF immersion. The present work sheds light on the primary fibroin structure of B.mori which probably plays a crucial role in regulating template-induced biomineralisation on the matrix. We also found that intrinsic material properties such as surface roughness, geometry, specific surface area, tortuosity and secondary conformation exert influence in modulating the extent of mineralisation. Thus our work generates useful insights and warrants future studies to further investigate the potential of bone mimetic, silk/mineral nanocomposite matrices for orthopaedic applications.

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“…One way is to directly electrospin suspension solutions containing biodegradable polymer and inorganic nanoparticles to get composite nanofibers . The other method is to soak electrospun polymeric nanofibers in simulated body fluid (SBF) to get biomineralized nanofibers . In the former case, however, poor dispersion of inorganic nanoparticles in polymeric matrixes is remaining a primary obstacle in obtaining high performance composite nanofibers .…”

Section: Introductionmentioning

confidence: 99%

Regulating proliferation and differentiation of osteoblasts on poly(l-lactide)/gelatin composite nanofibers via timed biomineralization

Zhang

Cao

Lan

et al. 2016

J. Biomed. Mater. Res.

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Mimicking the natural bone extracellular matrix, biomineralized nanofibers are envisioned as good choices for bone regeneration. Herein, composite nanofibers composed of poly(L-lactide) (PLLA) and gelatin (50/50, w/w) were electrospun and soaked in a modified five times simulated body fluid (SBF) for 6-24 h. Along with the soaking time, the amounts of deposited minerals increased, and the minerals transformed from dicalcium phosphate dehydrate (DCPD) to hydroxyapatite (HA). Mineral dissolution and Ca(2+) ion release of these biomineralized nanofibers were investigated by putting them in deionized water or Hank's balanced salt solution. MC3T3-E1 osteoblasts were cultured in transwell chambers without contacting the materials or on the biomineralized nanofibers directly. In the noncontact culture, the released ions were found able to enhance osteogenic differentiation more significantly in comparison with cell proliferation. In the contact culture, all biomineralized nanofibers demonstrated strong ability in promoting both cell proliferation and osteogenic differentiation. Due to the fast dissolution of DCPD, the biomineralized nanofibers obtained from short SBF soaking was found to be inferior in enhancing osteogenic differentiation. Whereas the cells displayed high levels of alkaline phosphatase activity and collagen I synthesis when the material had abundant deposition of apatite. The results revealed that cell biological behaviors were synergistically influenced by the ionic dissolution products, the composition, and the morphology of biomineralized nanofibers, which could be regulated by timed biomineralization. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1968-1980, 2016.

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Surface coating of hydroxyapatite on silk nanofiber through biomineralization using ten times concentrated simulated body fluid and the evaluation for bone regeneration

Cited by 31 publications

References 27 publications

Electrospun biodegradable nanofibers scaffolds for bone tissue engineering

Electrospun biodegradable nanofibers scaffolds for bone tissue engineering

Elucidation of differential mineralisation on native and regenerated silk matrices

Regulating proliferation and differentiation of osteoblasts on poly(l-lactide)/gelatin composite nanofibers via timed biomineralization

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