Surface immobilized biochemical macromolecules studied by scanning Kelvin microprobe

Cheran, Larisa-Emilia; McGovern, Mark E.; Thompson, Michael

doi:10.1039/b003259j

Cited by 23 publications

(19 citation statements)

References 5 publications

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“…on metal conductors, 49 but has recently been applied to semiconductor and biological surfaces, including proteins and nucleic acids. [50][51][52][53] This is possible since the work function is a fundamental property of all matter, not just conducting metals. By monitoring changes in work function, it is possible to measure surface effects at the micro-and even nano-metric scale.…”

Section: Detection Techniquesmentioning

confidence: 99%

Label-free detection of neuron–drug interactions using acoustic and Kelvin vibrational fields

Cheran

Cheung

Chawaf

et al. 2007

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Kelvin and acoustic fields of high-frequency have been employed in the non-invasive investigation of immortalized hypothalamic neurons, in order to assess their response to different concentrations of specific drugs, toxins, a stress-reducing hormone and neurotrophic factors. In an analytical systems biology approach, this work constitutes a first study of living neuron cultures by scanning Kelvin nanoprobe (SKN) and thickness shear mode (TSM) acoustic wave techniques. N-38 hypothalamic mouse neurons were immobilized on the gold electrode of 9 MHz TSM acoustic wave devices and gold-coated slides for study by SKN. The neurons were exposed to the neurochemicals betaseron, forskolin, TCAP, and cerebrolysin. Signals were collected with the TSM in real-time mode, and with the SKN in scanning and real-time modes, as the drugs were applied at biologically significant concentrations. With the TSM, for all drugs, some frequency and resistance shifts were in the same direction, contrary to normal functioning for this type of instrument. Possible mechanisms are presented to explain this behaviour. An oscillatory signal with periodicity of approximately 2 min was observed for some neuron-coated surfaces, where the amplitude of these oscillations was altered upon application of certain neurotrophic factors. These two new techniques present novel and non-invasive electrodeless methods for detecting changes at the cellular level caused by a variety of neuroactive compounds, without killing or destroying the neurons.

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Section: Detection Techniquesmentioning

confidence: 99%

Label-free detection of neuron–drug interactions using acoustic and Kelvin vibrational fields

Cheran

Cheung

Chawaf

et al. 2007

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“…Further, its high voltage resolution, being about a few millivolts, allows the study of surface potential distributions or differences of a variety of thin films, including those possessing poor conductive properties. [14] Being a noncontact and non-destructive method, it does not pose the risk of desorbing or removing even weakly bound species from the surface. In addition, it does not require particular sample treatments, such as exposure to high electric fields or electron or photon beams.…”

Section: Introductionmentioning

confidence: 99%

Electronic Characterization of Organic Thin Films by Kelvin Probe Force Microscopy

2006

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This review highlights the potential of Kelvin probe force microscopy (KPFM) beyond imaging to simultaneously study structural and electronic properties of functional surfaces and interfaces. This is of paramount importance since it is well established that a solid surface possesses different properties than the bulk material. The versatility of the technique allows one to carry out investigations in a non‐invasive way for different environmental conditions and sample types with resolutions of a few nanometers and some millivolts. KPFM can be used to acquire a wide knowledge of the overall electronic and electrical behavior of a sample surface. Moreover, by KPFM it is possible to study complex electronic phenomena in supramolecular engineered systems and devices. The combination of such a methodology with external stimuli, e.g., light irradiation, opens new doors to the exploration of processes occurring in nature or in artificial complex architectures. Therefore, KPFM is an extremely powerful technique that permits the unraveling of electronic (dynamic) properties of materials, enabling the optimization of the design and performance of new devices based on organic‐semiconductor nanoarchitectures.

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“…In order to examine this effect, higher spatial resolution than confocal fluorescence microscopy, is mandatory. With respect to this requirement, we are currently evaluating other surface methods such as the scanning Kelvin microprobe, 14 which may offer a more complete assessment, and an interesting technique for correlation with TOF-SIMS.…”

Section: Resultsmentioning

confidence: 99%

Imaging TOF-SIMS analysis of oligonucleotide microarrays

Cheran¹,

Vukovich²,

Thompson³

2002

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Single strand thiolated oligonucleotide (25-mer) was printed onto chemically modified glass and silicon surfaces. Confirmation of the level of attachment attained in each case was effected through detection by conventional confocal fluorescence microscopy. Both positive-ion and negative ion imaging time-of-flight mass spectra were recorded for the visualization of micro-patterned oligonucleotide arrays. This represents the first report of such detection by this form of mass spectrometry on glass. Ultimately, we are interested in the possibility that imaging time-of-flight secondary ion mass spectrometry can discern the orientation and conformation of DNA strands present on the surface of a substrate.

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Surface immobilized biochemical macromolecules studied by scanning Kelvin microprobe

Cited by 23 publications

References 5 publications

Label-free detection of neuron–drug interactions using acoustic and Kelvin vibrational fields

Label-free detection of neuron–drug interactions using acoustic and Kelvin vibrational fields

Electronic Characterization of Organic Thin Films by Kelvin Probe Force Microscopy

Imaging TOF-SIMS analysis of oligonucleotide microarrays

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