Surface micropatterning with zirconia and calcium phosphate ceramics by micromoulding in capillaries

Barata, David; Resmini, Alessandro; Pereira, Daniel de Melo; Veldhuis, Sjoerd A.; Blitterswijk, Clemens A. van; Elshof, Johan E. ten; Habibović, Pamela

doi:10.1039/c5tb02027a

Cited by 9 publications

(10 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To assess cell shape on different substrates by SEM and fluorescence microscopy, the cells were cultured for 24 hours in BM. This time point, at which a sufficient number of cells was attached on the surface to allow analysis, but the cells were not confluent yet, was selected based on the previous work, which showed that the effect of surface microfeatures on cell shape occurred early, and the maintenance of this effect was dependent on the properties of the substrate [ 32 , 33 ].…”

Section: Methodsmentioning

confidence: 99%

Controlling Growth and Osteogenic Differentiation of Osteoblasts on Microgrooved Polystyrene Surfaces

et al. 2016

View full text Add to dashboard Cite

Surface topography is increasingly being recognized as an important factor to control the response of cells and tissues to biomaterials. In the current study, the aim was to obtain deeper understanding of the effect of microgrooves on shape and orientation of osteoblast-like cells and to relate this effect to their proliferation and osteogenic differentiation. To this end, two microgrooved polystyrene (PS) substrates, differing in the width of the grooves (about 2 μm and 4 μm) and distance between individual grooves (about 6 μm and 11 μm, respectively) were fabricated using a combination of photolithography and hot embossing. MG-63 human osteosarcoma cells were cultured on these microgrooved surfaces, with unpatterned hot-embossed PS substrate as a control. Scanning electron- and fluorescence microscopy analyses showed that on patterned surfaces, the cells aligned along the microgrooves. The cells cultured on 4 μm-grooves / 11 μm-ridges surface showed a more pronounced alignment and a somewhat smaller cell area and cell perimeter as compared to cells cultured on surface with 2 μm-grooves / 6 μm-ridges or unpatterned PS. PrestoBlue analysis and quantification of DNA amounts suggested that microgrooves used in this experiment did not have a strong effect on cell metabolic activity or proliferation. However, cell differentiation towards the osteogenic lineage was significantly enhanced when MG-63 cells were cultured on the 2/6 substrate, as compared to the 4/11 substrate or unpatterned PS. This effect on osteogenic differentiation may be related to differences in cell spreading between the substrates.

show abstract

Section: Methodsmentioning

confidence: 99%

Controlling Growth and Osteogenic Differentiation of Osteoblasts on Microgrooved Polystyrene Surfaces

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Right: SEM micrograph of MG63 cells cultured on patterned ceramic for 72 h. Scale bar: 20 µm (Adapted with permission. [131] Copyright 2016, Royal Society of Chemistry) microstructure or micron-scale biomaterial particles with defined geometry. The additive manufacturing of biomaterials using microfluidic print heads [111][112][113] is an important example of the use of microfluidics in biomaterials fabrication with a resolution that is often higher than that of other additive manu-facturing techniques.…”

Section: Production Of Microstructured and Microscale Biomaterials With Defined Shape On Chipmentioning

confidence: 99%

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Guttenplan

Birgani

Giselbrecht

et al. 2021

Adv Healthcare Materials

View full text Add to dashboard Cite

In recent years, the use of microfabrication techniques has allowed biomaterials studies which were originally carried out at larger length scales to be miniaturized as so-called "on-chip" experiments. These miniaturized experiments have a range of advantages which have led to an increase in their popularity. A range of biomaterial shapes and compositions are synthesized or manufactured on chip. Moreover, chips are developed to investigate specific aspects of interactions between biomaterials and biological systems. Finally, biomaterials are used in microfabricated devices to replicate the physiological microenvironment in studies using so-called "organ-on-chip," "tissue-on-chip" or "disease-on-chip" models, which can reduce the use of animal models with their inherent high cost and ethical issues, and due to the possible use of human cells can increase the translation of research from lab to clinic. This review gives an overview of recent developments at the interface between microfabrication and biomaterials science, and indicates potential future directions that the field may take. In particular, a trend toward increased scale and automation is apparent, allowing both industrial production of micron-scale biomaterials and high-throughput screening of the interaction of diverse materials libraries with cells and bioengineered tissues and organs.

show abstract

“…3) A schematic representation of the process of surface micropatterning with CaP ceramics by micromoulding in capillaries, including a) casting of polydimethylxyloxane (PDMS) and curing to develop a mould, b) air plasma treatment, c) infiltration of a CaP solution into the channels, d) drying and e) mold removal. Adapted with permission . 2015, the Royal Society of Chemistry.…”

Section: Role Of Physical Propertiesmentioning

confidence: 99%

“…Structural patterning has already been widely used for polymeric biomaterials, since polymers are more amenable to various patterning techniques as compared to brittle and hard ceramic materials. Nevertheless, several techniques, such as micromachining or laser ablation, have been used to obtain patterned surfaces in (bioinert) ceramics, although their accuracy is below 100 µm . The use of microfabrication technologies, such as soft lithography, allows for ceramic patterning with higher accuracy.…”

Section: Role Of Physical Propertiesmentioning

confidence: 99%

“…The authors proposed micropatterning as a method to decouple chemical from geometrical cues in CaP. A recent paper by Barata et al reported patterning of CaP (dicalcium phosphate anhydrous (DCPA) and β‐TCP) on silicon substrates using a soft lithography approach (Figure .3). The linear ceramic patterns consisted of elongated crystals with radially branching morphology, occupying the volume of the channels used for the infiltration of the mineral solution.…”

Section: Role Of Physical Propertiesmentioning

confidence: 99%

See 1 more Smart Citation

Deconvoluting the Bioactivity of Calcium Phosphate‐Based Bone Graft Substitutes: Strategies to Understand the Role of Individual Material Properties

Galván-Chacón

Habibović

2017

Adv Healthcare Materials

View full text Add to dashboard Cite

Calcium phosphate (CaP)-based ceramics are the most widely applied synthetic biomaterials for repair and regeneration of damaged and diseased bone. CaP bioactivity is regulated by a set of largely intertwined physico-chemical and structural properties, such as the surface microstructure, surface energy, porosity, chemical composition, crystallinity and stiffness. Unravelling the role of each individual property in the interaction between the biomaterial and the biological system is a prerequisite for evolving from a trial-and-error approach to a design-driven approach in the development of new functional biomaterials. This progress report critically reviews various strategies developed to decouple the roles of the individual material properties in the biological performance of CaP ceramics. It furthermore emphasizes on the importance of a comprehensive and adequate material characterization that is needed to enhance our knowledge of the property-function relationship of biomaterials used in bone regeneration, and in regenerative medicine in general.

show abstract

Surface micropatterning with zirconia and calcium phosphate ceramics by micromoulding in capillaries

Abstract: Micropatterning of silicon surface with bioinert yttria-stabilised zirconia or bioactive calcium phosphate ceramic by micromoulding in capillaries.

Cited by 9 publications

References 71 publications

Controlling Growth and Osteogenic Differentiation of Osteoblasts on Microgrooved Polystyrene Surfaces

Controlling Growth and Osteogenic Differentiation of Osteoblasts on Microgrooved Polystyrene Surfaces

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Deconvoluting the Bioactivity of Calcium Phosphate‐Based Bone Graft Substitutes: Strategies to Understand the Role of Individual Material Properties

Contact Info

Product

Resources

About