Survival and cell acquisition rates after preimplantation embryo biopsy: Use of two mechanical techniques and two mouse strains

Roudebush, William E.; Kim, Jong G.; Minhas, Brijinder S.; Dodson, Melvin G.

doi:10.1016/0002-9378(90)91321-3

Cited by 34 publications

(28 citation statements)

References 13 publications

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“…Mouse embryos are widely used as a model to develop techniques of blastomere biopsy and examine their safety (7,9,12). Both blastocyst formation (6,16) and hatching (17) were advocated as criteria to evaluate the influence of biopsy on the embryos.…”

Section: Discussionmentioning

confidence: 99%

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A simplified technique for embryo biopsy: Use of the same micropipette for zona drilling and blastomere aspiration

Chen

Chao

et al. 1997

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 99%

“…The purpose of our study was to simplify the blastomere biopsy by using a single micropipette for both zona drilling and blastomere aspiration. We used mouse embryos to examine the feasibility of this technique (7,9,12). Blastocyst formation (6,16) and hatching rate (17) were used as criteria to judge the safety of this technique.…”

Section: Introductionmentioning

confidence: 99%

A simplified technique for embryo biopsy: Use of the same micropipette for zona drilling and blastomere aspiration

Chen

Chao

et al. 1997

J Assist Reprod Genet

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“…Moreover, following the transfer of biopsied mouse pre-embryos to pseudopregnant recipients, the rate of fetal development is similar to that for control pre-embryos (Sasabe, 1993). No effect on their development to blastocyst stage has been reported in biopsy of two blastomeres from 8-cell pre-embryos (Roudebush et al, 1990).…”

Section: Discussionmentioning

confidence: 95%

Sexing ofin vitro-fertilized preimplantation mouse embryos by the PCR method

Yano

1993

Jap J Human Genet

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SummaryThe applicability of the dual PCR method to embryo sexing was examined with the aim of establishing a noninvasive method of preimplantation diagnosis for human genetic disorders. Mouse pre-embryos obtained by in vitro fertilization were studied. The Sry gene sequence and the myogenin sequence were amplified as the Y-specific and internal control sequences, respectively. Amplification of as little as 10 pg of mouse genomic DNA was possible with the dual PCR method, the sensitivity being 10-fold greater than that of the single PCR method. The sex was identified in 100% (24/24) and 96% (23/24) of the pre-embryos tested at the 16-and 4-cell stages, respectively. In addition, the sex of all four single blastomeres dissociated from 4-cell pre-embryos agreed in 76% (16/21) of the specimens tested and 94% (79/84) of dissociated blastomeres could be sexed. The sex of single blastomeres biopsied from pre-embryos at the 8-cell stage could be identified. After transfer of 13 male and 25 female sexed pre-embryos, six viable fetuses were obtained. Histological examination showed that all these fetuses were of the predicted sex.Sexing of biopsied single blastomeres by the dual PCR method was rapid and reliable, suggesting its feasibility for preimplantation diagnosis of in vitro fertilized human pre-embryos.

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“…Other reports using similar displacement techniques have shown 96% intact blastomere after acid Tyrode's digestion (5) and 59% intact blastomeres using partial zona dissection (3). Furthermore, Roudebush et al (3) showed that blastomere integrity was higher using a displacement method compared with direct aspiration into a micropipette (59% vs. 22%, respectively).…”

Section: Discussionmentioning

confidence: 97%

“…However, this method is very difficult to master and may cause rupture of the biopsied blastomere and damage to the embryo if not done correctly. Other methods have also been suggested, such as displacing the blastomere though the ZP with medium (3)(4)(5) or pushing against the ZP to squeeze the blastomere out (6)(7)(8). Although these biopsy techniques show similar embryo survival, the displacement method has shown higher survival of the biopsied blastomere (3).…”

mentioning

confidence: 99%