Synthesis and Functional Characterization of MAF-1A Peptide Derived From the Larvae of Housefly,Musca domestica(Diptera: Muscidae)

Abstract

The Musca domestica antifungal peptide-1A (MAF-1A peptide) from housefly larvae was synthesized by solid-phase synthesis technique, and antiviral, antioxidant, and antifungal properties were evaluated in this study. Present results indicated that it could significantly inhibit the infection of influenza virus HN, Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), and Bombyx mori nuclear polyhydrosis virus (BmNPV), which displayed excellent virucidal activities. Antioxidant results demonstrated that the MAF-1A peptide had effective scavenging activity for hydroxyl and superoxide radicals, which were similar to that of ascorbic acid. Besides, antifungal results showed that it can also significantly inhibit the growth of four fungi, and the half inhibitory concentrations (IC) values were ∼59.3, 84.2, 144.9, and 48.5 μg/ml, respectively, highlighting an important role of MAF-1A peptide in the defense of M domestica against pathogenic microorganisms. These results revealed that the MAF-1A peptide from housefly larvae has great potential as a natural ingredient for the exploitation of antiviral and antifungal therapeutic agents, avoiding abuse of chemical agents and environmental pollution.

Transcriptional Responses of Candida albicans to Antimicrobial Peptide MAF-1A

“…MAF-1A peptide was derived from the structure domain of MAF-1 and contained 26 amino acid residues (KKFKETADKLIESAKQQLESLAKEMK). MAF-1A showed its remarkable antifungal effect (Luo et al, 2013; Zhou et al, 2016), but the detailed antifungal actions of MAF-1A remain unclear.…”

Identification of proteins in housefly (Musca domestica) larvae powder by LC-MS/MS and their potential medical relevance

“…Zhou et al revealed that the antifungal peptide-1A from HL has excellent antiviral activities and antifungal activities. 43 The antifungal peptide-1 identied in this study may be one of the material basis for HL to exert its antibacterial effect. In order to carry out further in-depth research, we published its peptide sequences (Table 2).…”

Image recognition of automatic evisceration of Cherry Valley ducks and biological activities of protein extracts isolated from the duck meat

“…Superoxide anion scavenging capacity was carried out with reference to the method by Zhou et al (). First, the mixed reaction solution contained the protein samples (1 mL, 0.0625, 0.125, 0.25, 0.5, 1.0, and 2.0 mg/mL), phenazine‐methosulfate (1 mL, 30 μM), nicotinamide adenine dinucleotide (1 mL, 338 μM), and nitroblue tetrazolium (1 mL, 72 μM) in PBS buffer (0.1 M, pH 7.4).…”