Synthesis of tethered poly(N-isopropylacrylamide) for detection of breast cancer recurrence DNA

Chen, Jem-Kun; Li, Junyan

doi:10.1016/j.jcis.2011.03.063

Cited by 23 publications

(9 citation statements)

References 40 publications

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“…This type of hydrogel has a crosslinked network structure in which the stimuli-responsive component is in the polymer chains, which causes dramatic swelling/deswelling according to the change in stimuli. Other forms of stimuli-responsive hydrogels could be reversibly transformed to solutions due to changes in environmental stimuli, showing solution-gelation (sol-gel) transition by altering the hydrophobic interactions of cross-linked areas in an aqueous system [175]. Therefore, this type of stimulus-responsive polymer has been developed for a phase change rather than a dimension change, to be used, for example, as injectable hydrogels [176].…”

Section: Application Of Nanostructured Stimuli-responsive Hydrogels (mentioning

confidence: 99%

Fabrications and Applications of Stimulus-Responsive Polymer Films and Patterns on Surfaces: A Review

2014

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In the past two decades, we have witnessed significant progress in developing high performance stimuli-responsive polymeric materials. This review focuses on recent developments in the preparation and application of patterned stimuli-responsive polymers, including thermoresponsive layers, pH/ionic-responsive hydrogels, photo-responsive film, magnetically-responsive composites, electroactive composites, and solvent-responsive composites. Many important new applications for stimuli-responsive polymers lie in the field of nano- and micro-fabrication, where stimuli-responsive polymers are being established as important manipulation tools. Some techniques have been developed to selectively position organic molecules and then to obtain well-defined patterned substrates at the micrometer or submicrometer scale. Methods for patterning of stimuli-responsive hydrogels, including photolithography, electron beam lithography, scanning probe writing, and printing techniques (microcontact printing, ink-jet printing) were surveyed. We also surveyed the applications of nanostructured stimuli-responsive hydrogels, such as biotechnology (biological interfaces and purification of biomacromoles), switchable wettability, sensors (optical sensors, biosensors, chemical sensors), and actuators.

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Section: Application Of Nanostructured Stimuli-responsive Hydrogels (mentioning

confidence: 99%

Fabrications and Applications of Stimulus-Responsive Polymer Films and Patterns on Surfaces: A Review

2014

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“…XPS was employed to determine the chemical compositions of the Au surfaces at various stages during the surface modification process as well as the presence of the grafted PNIPAAm- b -ssDNA copolymer brushes [ 8 ]. After polymerization for 20 h, the chemical compositions determined of 427 nm thick layer of PNIPAAm-N 3 and of PNIPAAm- b -ssDNA brushes at temperatures below and above the LCST was shown (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…One of the most highly characterized thermosensitive amphiphiles is poly( N -isopropylacrylamide) (PNIPAAm), which contains hydrophilic amido groups and hydrophobic isopropyl groups [ 6 , 7 ]. PNIPAAm is soluble in water up to its lower critical solution temperature (LCST), which was approximately 31–32 °C [ 8 ], because of amido groups forms hydrogen bonds (HB) with the surrounding water molecules [ 9 , 10 ]. Integration DNA with polymers can modulate the properties of biological entities, forming new hybrid materials well known as self-assembled supramolecules under thermal or pH stimulus [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of device with poly(N-isopropylacrylamide)-b-ssDNA copolymer brush for resistivity study

et al. 2017

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In this study, we grafted bromo-terminated poly(N-isopropylacrylamide) (PNIPAAm) brushes onto thin gold films deposited on silicon, and then reacted with NaN3 to produce azido-terminated PNIPAAm brushes. A probe sequence of single-stranded DNA (ssDNA) with a 4-pentynoic acid succinimidyl ester unit was grafted onto the azido-terminated PNIPAAm brushes through a click reaction, resulting in the formation of block copolymer brushes. The PNIPAAm-b-ssDNA copolymer brushes formed supramolecular complexes stabilized by bio-multiple hydrogen bonds (BMHBs), which enhanced the proton transfer and thereby decreased the resistivity of the structures. In addition, the optimal operation window for DNA detection ranges from 0 to 0.2 M of NaCl concentration. Therefore, the specimens were prepared in the PBS solution at 150 mM NaCl concentration for target hybridization. The supramolecular complex state of the PNIPAAm-b-ssDNA copolymer brushes transformed into the phase-separated state after the hybridization with 0.5 ng/µL of its target DNA sequence owing to the competition between BMHBs and complementary hydrogen bonds. This phase transformation of the PNIPAAm and probe segments inhibited the proton transfer and significantly increased the resistivity at 25 °C. Moreover, there were no significant changes in the resistivity of the copolymer brushes after hybridization with the target sequence at 45 °C. These results indicated that the phase-separated state of the PNIPAAm-b-ssDNA copolymer brushes, which was generally occurred above the LCST, can be substantially generated after hybridization with its target DNA sequence. By performing the controlled experiments, in the same manner, using another sequence with lengths similar to that of the target sequence without complementarity. In addition, the sequences featuring various degrees of complementarity were exploited to verify the phase separation behavior inside the PNIPAAm-b-ssDNA copolymer thin film.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-017-0303-4) contains supplementary material, which is available to authorized users.

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“…In the past decade, sequence-specic detection of DNA has garnered considerable interest due to its application in clinical diagnosis, gene expression analysis, and biomedical studies. [1][2][3] Identication and quantication of specic DNA sequences related to disease is the purpose of DNA detection. Mostly, detection of DNA has been from DNA sequencing 4 and DNA microarrays.…”

Section: Introductionmentioning

confidence: 99%

Identification of DNA single-base mismatches by resistivity of poly(N-isopropylacrylamide)-block-ssDNA copolymer brush films at dual temperatures

et al. 2017

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Synthesis of tethered poly(N-isopropylacrylamide) for detection of breast cancer recurrence DNA

Cited by 23 publications

References 40 publications

Fabrications and Applications of Stimulus-Responsive Polymer Films and Patterns on Surfaces: A Review

Fabrications and Applications of Stimulus-Responsive Polymer Films and Patterns on Surfaces: A Review

Fabrication of device with poly(N-isopropylacrylamide)-b-ssDNA copolymer brush for resistivity study

Identification of DNA single-base mismatches by resistivity of poly(N-isopropylacrylamide)-block-ssDNA copolymer brush films at dual temperatures

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