Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales

Abstract

The relatedness among methanol-assimilating yeasts assigned to the genus Ogataea and neighboring taxa (Phylum Ascomycota, Subphylum Saccharomycotina, Class Saccharomycetes, Order Saccharomycetales) was determined from phylogenetic analyses of gene sequences for nuclear large and small subunit (SSU) rRNAs, translation elongation factor-1alpha and mitochondrial SSU rRNA. On the basis of the analyses, Williopsis salicorniae and seven species of Pichia are proposed for transfer to the genus Ogataea, which has been emended, and Pichia angophorae, a nonhyphal species, is proposed for transfer to the mycelium forming genus Ambrosiozyma. Pichia toletana and Pichia xylosa form an independent lineage and are assigned to the genus Peterozyma, which is newly proposed.

Ogataea kolombanensis sp. nov., Ogataea histrianica sp. nov. and Ogataea deakii sp. nov., three novel yeast species from plant sources

“…The differing results can partly be explained by the fact that the fragments of the EF-1a gene analysed by Kurtzman & Robnett (2010) and in the current study are not exactly the same. Approximately 930 and 820 nt were amplified by Kurtzman & Robnett (2010) and during the present study, respectively, and about 640 bp are overlapping.…”

Description of Ambrosiozyma oregonensis sp. nov., and reassignment of Candida species of the Ambrosiozyma clade to Ambrosiozyma kashinagacola f.a., comb. nov., Ambrosiozyma llanquihuensis f.a., comb. nov., Ambrosiozyma maleeae f.a., comb. nov., Ambrosiozyma pseudovanderkliftii f.a., comb. nov., and Ambrosiozyma vanderkliftii f.a., comb. nov.

“…An earlier phylogenetic analysis of the Ambrosiozyma clade determined with concatenated gene sequences from LSU rRNA, SSU rRNA, EF-1a and mitochondrial SSU rRNA also placed A. angophorae within the Ambrosiozyma clade and showed C. llanquihuensis to be a well-supported basal member of the clade (Kurtzman & Robnett, 2010). For reference, Ogataea minuta NRRL Y-411 T , the type species of the genus Ogataea, was included in the present analysis (Figs 1 and S1).…”

“…However, inclusion of this single species of Ogataea does not convey the large separation between Ogataea and Ambrosiozyma that is shown when the majority of Ogataea species are included in an analysis ( Fig. 1; Kurtzman & Robnett, 2010).…”

“…Members of the Ambrosiozyma clade appear worldwide in distribution with species reported from South Africa (van der Walt, 1972), Europe (Smith, 2011), South America (Ramírez & González, 1984), North America (Smith, 2011) and Asia (Endoh et al, 2011;Nakase et al, 2011). Multigene phylogenetic analyses have placed Ambrosiozyma as an early diverging member in a clade that includes the genera Pichia, Ogataea, Kregervanrija and Saturnispora (Kurtzman & Robnett, 2010, 2013. Included in the Ambrosiozyma clade are several non-ascospore-forming species that are classified as Candida kashinagacola, Candida llanquihuensis, Candida maleeae, Candida pseudovanderkliftii and Candida vanderkliftii.…”

Characterization of a New Clinical Yeast Species, Candida tunisiensis sp. nov., Isolated from a Strain Collection from Tunisian Hospitals

“…Including JEY63, this alignment included 435 taxa. A second data set (4 loci/79 taxa) was built combining JEY63 sequences of the 18S, 26S, 12S, and TEF1 loci with sequence data of the same loci sampled in GenBank for 77 taxa representative of the major clades identified in the Saccharomycetes in previously published phylogenetic studies (22)(23)(24) and for an outgroup, Schizosaccharomyces pombe (see Table S1 in the supplemental material). Alignment of nucleotide sequences was performed manually using the MacClade 4.06 editor (25).…”