Tapered Microfluidic for Continuous Micro-Object Separation Based on Hydrodynamic Principle

Ahmad, Ida Laila; Ahmad, Mohd Ridzuan; Takeuchi, Masaru; Nakajima, Masahiro; Hasegawa, Yasuhisa

doi:10.1109/tbcas.2017.2764118

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…The "hydrodynamic separations" are supported by a coupling mechanism and sedimentation, which help to achieve the required sample purity. The process is supported by technology in the form of computer-aided design (CAD) [190,191] and finite element analysis (FEA) [192,193]. The device is fabricated using soft lithography.…”

Section: Cell Sorting and Single-cell Analysismentioning

confidence: 99%

Microfluidics for Peptidomics, Proteomics, and Cell Analysis

et al. 2021

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Microfluidics is the advanced microtechnology of fluid manipulation in channels with at least one dimension in the range of 1–100 microns. Microfluidic technology offers a growing number of tools for manipulating small volumes of fluid to control chemical, biological, and physical processes relevant to separation, analysis, and detection. Currently, microfluidic devices play an important role in many biological, chemical, physical, biotechnological and engineering applications. There are numerous ways to fabricate the necessary microchannels and integrate them into microfluidic platforms. In peptidomics and proteomics, microfluidics is often used in combination with mass spectrometric (MS) analysis. This review provides an overview of using microfluidic systems for peptidomics, proteomics and cell analysis. The application of microfluidics in combination with MS detection and other novel techniques to answer clinical questions is also discussed in the context of disease diagnosis and therapy. Recent developments and applications of capillary and microchip (electro)separation methods in proteomic and peptidomic analysis are summarized. The state of the art of microchip platforms for cell sorting and single-cell analysis is also discussed. Advances in detection methods are reported, and new applications in proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices and determination of their physicochemical parameters are highlighted.

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Section: Cell Sorting and Single-cell Analysismentioning

confidence: 99%

Microfluidics for Peptidomics, Proteomics, and Cell Analysis

et al. 2021

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“…The entire fluidic network was simplified to five resistive elements: on-chip Leg 1, cantilever Leg 1, on-chip Leg 2, cantilever Leg 2 and apertures & pyramid. 35,41,42 Their relative contribution to the total resistance of the probe is given in Fig. 3.…”

Section: Fluidic Resistancementioning

confidence: 99%

Volume and concentration dosing in picolitres using a two-channel microfluidic AFM cantilever

Verlinden

Madadelahi

Sarajlic³

et al. 2020

Nanoscale

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“…However, centrifugation requires professional equipment, laboratory facilities, and operators, while also being expensive and not suitable for point-of-care testing (POCT) [ 6 , 7 ]. Consequently, methods to separate biological particles have been extensively explored, with microfluidic technology [ 8 , 9 , 10 ] emerging as a promising means for separating particles.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis Separation of Platelets Using a Novel Zigzag Microchannel

et al. 2020

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Platelet separation and purification are required in many applications including in the detection and treatment of hemorrhagic and thrombotic diseases, in addition to transfusions and in medical research. In this study, platelet separation was evaluated using a novel zigzag microchannel fluidic device while leveraging a dielectrophoresis (DEP) electric field using the COMSOL multiphysics software package and additional experimentation. The zigzag-shaped microchannel was superior to straight channel devices for cell separation because the sharp corners reduced the required horizontal distance needed for separation and also contributed to an asymmetric DEP electric field. A perfect linear relationship was observed between the separation distance and the corner angles. A quadratic relationship (R2 = 0.99) was observed between the driving voltage and the width and the lengths of the channel, allowing for optimization of these properties. In addition, the voltage was inversely proportional to the channel width and proportional to the channel length. An optimal velocity ratio of 1:4 was identified for the velocities of the two device inlets. The proposed device was fabricated using laser engraving and lithography with optimized structures including a 0.5 mm channel width, a 120° corner angle, a 0.3 mm channel depth, and a 17 mm channel length. A separation efficiency of 99.4% was achieved using a voltage of 20 V and a velocity ratio of 1:4. The easy fabrication, lower required voltage, label-free detection, high efficiency, and environmental friendliness of this device make it suitable for point-of-care medicine and biological applications. Moreover, it can be used for the separation of other types of compounds including lipids.

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Tapered Microfluidic for Continuous Micro-Object Separation Based on Hydrodynamic Principle

Cited by 5 publications

References 48 publications

Microfluidics for Peptidomics, Proteomics, and Cell Analysis

Microfluidics for Peptidomics, Proteomics, and Cell Analysis

Volume and concentration dosing in picolitres using a two-channel microfluidic AFM cantilever

Dielectrophoresis Separation of Platelets Using a Novel Zigzag Microchannel

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