Técnica para o estudo da anatomia da epiderme foliar de batata

Segatto, Fernanda Bastos; Bisognin, Dílson Antônio; Benedetti, Marlova; Costa, Liege Camargo da; Rampelotto, Marcos Vicente; Nicoloso, Fernando Teixeira

doi:10.1590/s0103-84782004000500042

Cited by 85 publications

(43 citation statements)

References 4 publications

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“…The number of glandular and non-glandular trichomes on these three apical leafl ets was also registered in order to correlate whitefl y oviposition with trichome density. Trichome numbers were determined using the epidermal fi ngerprint technique (Segatto et al 2004), and trichome density was calculated according to McAuslane (1996).…”

Section: Methodsmentioning

confidence: 99%

Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes

Oriani¹,

Vendramim²

2010

Neotrop. entomol.

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-Brazil is one of the world's largest tomato producer, but considerable part of the production is lost due to the attack of Bemisia tabaci (Genn.) B biotype. Resistant germoplasm plants can be an important method for B. tabaci control in integrated pest management approaches. The attractiveness and ovipositional preference of B. tabaci for 17 tomato genotypes were evaluated in a free-choice test. Trials were set up in a randomized block design with ten replicates. Each replicate (one tomato plant per pot) was placed in a cage (80 x 50 x 50 cm) and infested with 1,000 adults during four days. Linear correlation tests were applied between the number of insects and eggs and number of trichomes in each tomato genotype. LA716, LA444-1 and PI134418 genotypes were the least attractive, while the 'Santa Clara' was the most attractive; PI134417 trapped the largest number of adults. LA716 genotype (4.1 eggs/leafl et and 2.1 cm 2 /eggs per leafl et) was the least preferred for whitefl y oviposition; NAV1062, 'Fanny', LA1335, 'Santa Clara' and IAC294 were the most preferred genotypes. The glandular trichomes density was negatively correlated with whitefl y's attractiveness and oviposition per leafl et and per leaf, and positively with the number of trapped insects. The non-glandular trichomes density was negatively correlated with the number of trapped insects and positively with whitefl y's oviposition per cm 2 /leafl et and per cm 2 /leaf. LA716 had high antixenosis level (ovipositional nonpreference) toward B. tabaci B biotype related with type IV glandular trichome.

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Section: Methodsmentioning

confidence: 99%

Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes

Oriani¹,

Vendramim²

2010

Neotrop. entomol.

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“…Despite wide use, anatomical studies apply laborious techniques (Macedo, 1997) that make work hard and long time-consuming. Segatto et al (2004) demonstrated a study of leaf epidermis anatomy in potato clones (Solanum tuberosum L.) by an alternative technique, the printing out of leaf epidermis with cianoacrilate ester universal instantaneous adhesive. The aim of this work was to identify some Lauraceae tree species from remnants of Seasonal Forests at Serra Geral formation, Rio Grande do Sul state, applying the epidermical printing technique with cianocrilate ester as an alternative anatomic tool.…”

Section: Introductionmentioning

confidence: 99%

Leaf surface characters applied to Lauraceae taxonomy in a Seasonal Forest of Southern Brazil

Ceolin

Rosito²,

Canto-Dorow³

2009

Braz. arch. biol. technol.

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The goal of this work was to test if the macro and micro morphological analysis of the leaf surface could provide vegetative diagnostic characters for some of the most common Lauraceae species in Southern Brazilian Seasonal Forests. The leaf printing technique with universal instantaneous adhesive was used for the epidermical microscopical analysis and external macroscospical analyses of leave were made. Microscopic evaluation revealed the visibility and contours of anticlinal walls of epidermical cell and stomata and shape of guard-cells. Macroscopic evaluation showed the absence or presence of characters such as hairiness, domatia and scents.The results showed that analyzed characters, together with other diagnostic characteristics, could contribute in taxonomic delimitation of some common Lauraceae species in the Southern Brazil.

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“…Para ilustração da superfície epidérmica, foram retiradas amostras das folhas e realizadas impressões paradérmicas do material (Segatto et al, 2004).…”

Section: Methodsunclassified

Caracterização anatômica de folha, colmo e rizoma de Digitaria insularis

Machado

Meira²,

Ferreira³

et al. 2008

Planta daninha

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RESUMO -A anatomia da folha, de colmo e do rizoma de Digitaria insularis foi investigada, visando identificar características que possam estar relacionadas à sua tolerância ao glyphosate. Sementes e rizomas de plantas adultas foram coletadas em campo, em área de plantio direto, onde o herbicida glyphosate vem sendo utilizado repetidamente há vários anos. As plantas provenientes dessas sementes e desses rizomas foram cultivadas em vasos com capacidade de 3 L, contendo solo de textura média, em casa de vegetação. Quando as plantas atingiram o estádio fenológico de pré-florescimento, foram coletadas três folhas totalmente expandidas por planta, entre o terceiro e o quinto nó. Simultaneamente, coletaram-se fragmentos dos rizomas e os entrenós recobertos pelas bainhas das folhas amostradas. As amostras foram fixadas em FAA 50 e estocadas em etanol, para obtenção de cortes histológicos e montagem das lâminas, conforme metodologia usual. Para obtenção do índice estomático e da densidade estomática nas superfícies da epiderme, amostras das folhas foram diafanizadas, conforme método utilizado usualmente. Verificou-se que plantas provenientes de rizomas apresentavam maior índice estomático e maior número de estômatos por mm 2 , maior espessura na epiderme das faces adaxial e abaxial e maior espessura da lâmina foliar. Foi observada coloração intensa nos rizomas submetidos ao Lugol, indicando presença de grande quantidade de amido, independentemente da origem do material.Palavras-chave: capim-amargoso, anatomia, glyphosate, amido.ABSTRACT -The anatomy of the leaf, stem and rhizome of Digitaria insularis was studied, evaluating characteristics that could be related to its tolerance to glyphosate. Seeds and rhizomes of adult plants were collected in the field, area of no-till system, where the herbicide glyphosate had been repeatedly used for several years. The plants derived from these seeds and rhizomes were cultivated in 0.003 m 3 vases, containing soil, under a greenhouse conditions. When the plants reached the preflowering stage, three totally expanded leaves were collected per plant, between the third and fifth nodes. Fragments of the internodes covered by the sheaths of the sampled leaves were collected together with the rhizomes. The samples were fixed in FAA 50 and embedded in ethanol to obtain histological sections and mount the slider, according to the methodology. Leaf samples were diaphanized, following the usual methodology, to obtain the stomatal index and stomatal density at the epidermal surfaces. Plants derived from rhizomes had higher stomatal index and larger number of stomata per mm 2 , thicker adaxial and abaxial epidermis faces and thicker leaf lamina. The intense coloration in the rhizomes treated with lugol indicated the presence of a great amount of starch, regardless of the origin of the material.

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Técnica para o estudo da anatomia da epiderme foliar de batata

Cited by 85 publications

References 4 publications

Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes

Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes

Leaf surface characters applied to Lauraceae taxonomy in a Seasonal Forest of Southern Brazil

Caracterização anatômica de folha, colmo e rizoma de Digitaria insularis

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