Tetraploid induction of Mitracarpus hirtus L. by colchicine and its characterization including antibacterial activity

Pansuksan, Kanoktip; Sangthong, Ratchada; Nakamura, Ikuo; Mii, Masahiro; Supaibulwatana, Kanyaratt

doi:10.1007/s11240-014-0447-y

Cited by 35 publications

(12 citation statements)

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“…One of the immediate and obvious consequences of polyploidy in plants is an increase in cell size which in turn leads to enlarged plant organs, a phenomenon termed gigas effect (Acquaah, 2007;Levin, 2002). For example, the volume of tetraploid cells usually is about twice that of their diploid progenitors (Acquaah, 2007;Emsweller and Ruttle, 1941;Levi et al,2002;Schepper et al, 2001).As the enlargement of the stomata in this observation point out the most of the cholchicine treated polyploid plant production compare with the diploids, the length and width of guard cells increased by 48.25% and 52.62%, respectively in previous studies (Przywara et al, 1988;Cohen and Yao 1996;Pansuksan et al, 2014;Quin et al, 2016).…”

Section: Acclimatization and Transferring Of Plantssupporting

confidence: 55%

In vitro induction of tetraploids from Exacum ritigalensis (Gentianaceae)

Perera

Dahanayake

Senanayake

et al. 2016

Trop Agric Res & Ext

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Exacum ritigalensis (Gentianaceae) (Sinhalese: Binara or Ginihiriya) has a potential to develop as a potted plant by improving the size of the flowers and inflorescence. The aim of the study was to establish an efficient and effective system for the production of tetraploid plants through colchicine treatment. Single nodal cuttings (0.5 cm) from aseptic plantlets were established on Murashige and Skoog basal medium (MS) supplemented with 2 mgL -1 BAP and different colchicine concentrations (0, 10, 20, 30, 40 and 50 mg -1 ). They were kept in different durations (0, 7, 14 21 and 28 days) and transferred into 10ml of MS basal medium containing 2 mgL -1 BAP. After one month, they were transferred to MS medium containing 2mgL -1 Indole-3-butyric acid (IBA) for rooting. Then root tips were collected from treated plants to observe chromosome number. Afterwards the selected diploid and tetraploid plants were acclimatized and transferred to field to observe the morphological characters. Factorial Complete Randomized Design (FCRD) with 10 replicates was used for each treatment and statistical analysis was carried out using Duncan's Multiple Range Test of SAS program (9.1.3).The highest percentage of tetraploid plantlets (26.6%) was observed from nodal cuttings treated with MS basal medium with 2mgL -1 BAP + 30mgL -1 colchicine for seven days. The chromosome number of diploid and tetraploid E. ritigalensis determined as 28 and 56 respectively. Cells of the tetraploid plants were comparatively larger cells than diploids plants. The stomata size of the tetraploid (2.96*2.27 μm 2 ) were larger than those of the diploids (1.94*1.48μm 2 ). Leaf shape of diploids was lanceolate while it was ovate or narrowly elliptic in tetraploids. Flower petals of diploid plants were overlapping along their entire length while in tetraploids flower petals were not overlapping. The plants height was significantly higher in diploid (67.00±9 cm) than in tetraploid (38.00±4 cm). Newly produced polyploids of E. ritigalensis have a potential to popularize as a potted plant in the floriculture trade.

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Section: Acclimatization and Transferring Of Plantssupporting

confidence: 55%

In vitro induction of tetraploids from Exacum ritigalensis (Gentianaceae)

Perera

Dahanayake

Senanayake

et al. 2016

Trop Agric Res & Ext

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“…Colchicine has been found to have a significant effect on polyploid induction and is widely used for inducing polyploidy in plants (Tang et al 2010) because it can effectively arrests mitosis at the anaphase stage (Kunitake et al 1998; Kermani et al 2003). Until now, numerous researchers reported that germplasm resources have been innovated using polyploidization techniques in many crop or medicinal plants (Ahloowalia 1967; Kermani et al 2003; Tang et al 2010; Ye et al 2010; Kanoktip et al 2014) including F. esculentum (Zhu and Gao 1988; Lian and Chen 2013). Limited works regarding tartary buckwheat, polyploidy induction works have been reported (Hou et al 2002; Zhou et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Fagopyrum tataricum (L.) Gaertn

Wang

Sheng

Wen³

et al. 2017

Bot Stud

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BackgroundTartary buckwheat are very popular as an important functional food material and its cultivation is very widespread in our whole world, but there obviously lack works in the researches of genetic breeding for agricultural and medicinal utilization. The aim of this study is to obtain good germplasm resources for agricultural and medicinal use of tartary buckwheat (Fagopyrum tataricum) by inducing the tetraploid plants.ResultsFour cultivars of F. tataricum, that is, Qianwei 2#, Jinku 2#, Chuanqiao 1#, and Liuqiao 1# were selected to experiment. The tips of seedlings with two true leaves were treated by 0.25% (w/v) colchicine solution for 48, 72, and 96 h, respectively. The chromosome number of treated plants was determined by metaphase chromosome counting of root tip cells and PMCs (pollen mother cells) meiosis observation. Tetraploid induction successfully occurred in all three treatments with an efficiency ranging from 12.13 to 54.55%. The chromosome number of the diploid plants was 2n = 2x = 16, and that of the induced tetraploid plants was 2n = 4x = 32. The typical morphological and physiological qualities were compared between the control diploid and corresponding induced tetraploid plants. Results showed that the induced tetraploid plants had obviously larger leaves, flowers, and seeds. Moreover, the content of seed protein and flavonoid were also increased in the tetraploid plants. The pollen diameter and capsule size of diploid plants were significantly smaller than those of tetraploid plants.Conclusion Fagopyrum tataricum can be effectively induced into tetraploids by colchicines. The tetraploid induction can produce valuable germplasm resources for breeding and is a practicable breeding way in F. tataricum.

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“…These findings suggest that it is possibly feasible to extend the planting area of Trollius chinensis by induction of polyploid plants to improve tolerance to environmental stresses and enhance its resistance to a warmer climate. Colchicine, as one of the doi: 10.17221/89/2015-CJGPB efficient chemical agents of chromosome doubling, has been successfully used to modify the chromosome numbers in diverse plant species (Takamura & Miyajina 1996;Kermani et al 2004;Liu et al 2007Liu et al , 2013Pansuksan et al 2014).…”

mentioning

confidence: 99%

“…These findings revealed that the genome modifications of Trollius chinensis can largely alter the stomata properties. In previous studies, many researchers (Przywara et al 1988;Cohen & Yao 1996;Pansuksan et al 2014) also examined bigger stomatal cells in polyploids than in diploids. Thus, the size of stomatal guard cells seems to be an important determination marker to distinguish polyploid plants of Trollius chinensis.…”

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confidence: 99%

Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine

Zhang¹,

Zhang²,

Li³

et al. 2016

Czech J. Genet. Plant Breed.

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Zhang Q., Zhang F., Li B., Zhang L., Shi H. (2016): Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine. Czech J. Genet. Plant Breed., 52: 34-38.Trollius chinensis Bunge is one of the important ornamental and medicinal plants cultivated in China. In this study, the tetraploid plants of Trollius chinensis were successfully induced by colchicine and their morphological, cytological and fertility characteristics were investigated. The results indicated that the tetraploid species of Trollius chinensis (2n = 4x = 32) was efficiently induced from the diploid species (2n = 2x = 16) by colchicine under 0.05% to 0.2% concentrations, with the best induction under treatment combinations of 0.1%, 24h duration and 0.2%, 12h duration. In comparison with the diploid plants, the tetraploid plants exhibited dramatically varied phenotypic features, showing increased leaf length and width, petiole, flower and pollen diameters. In addition, they also exhibited increased stomatal size and chloroplast numbers in guard cells. Tetraploidy reduced the pollen fertility. Nevertheless, the maintenance of female fertility makes the tetraploid a potential germplasm for further polyploid breeding. Our investigation provides a potential design for an effective pathway to generate varieties of this plant species with chromosome doubling.

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Tetraploid induction of Mitracarpus hirtus L. by colchicine and its characterization including antibacterial activity

Cited by 35 publications

References 37 publications

<i>In vitro</i> induction of tetraploids from <i>Exacum ritigalensis</i> (Gentianaceae)

<i>In vitro</i> induction of tetraploids from <i>Exacum ritigalensis</i> (Gentianaceae)

Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Fagopyrum tataricum (L.) Gaertn

Production of tetraploid plants of Trollius chinensis Bunge induced by colchicine

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