The antimicrobial peptide aureocin A53 as an alternative agent for biopreservation of dairy products

Fagundes, Patrícia Carlin; Farias, Felipe Miceli de; Santos, O.C.S.; Oliveira, Naira Elane Moreira de; Paz, Juliana Aparecida Souza da; Ceotto-Vigoder, Hilana; Alviano, Daniela Sales; Romanos, Marcel; Bastos, M. C. F.

doi:10.1111/jam.13189

Cited by 21 publications

(10 citation statements)

References 44 publications

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“…Lacticin Q displays strong bioactivity even at nanomolar concentrations whereas many other non-leaderless bacteriocins are active at a relatively higher concentration ( Fujita et al, 2007 ). Although some leaderless bacteriocins are produced by a number of strains that are often associated with pathogenicity and having structural similarity with hemolytic peptides and toxins (i.e., aureocin A53 and A70 are produced by S. aureus ), these leaderless bacteriocins have been demonstrated to have no cytotoxic and hemolytic activities, thus underlining the safety of their application ( Fagundes et al, 2016a , b ). Similar observation was manifested by enterocin DD14 (identical to enterocins 7 and MR10) when it was evaluated for its cytotoxicity against intestinal epithelial cell line IPEC-1 ( Caly et al, 2017 ).…”

Section: Applications Challenges and Prospectsmentioning

confidence: 99%

Circular and Leaderless Bacteriocins: Biosynthesis, Mode of Action, Applications, and Prospects

2018

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Bacteriocins are a huge family of ribosomally synthesized peptides known to exhibit a range of bioactivities, most predominantly antibacterial activities. Bacteriocins from lactic acid bacteria are of particular interest due to the latter’s association to food fermentation and the general notion of them to be safe. Among the family of bacteriocins, the groups known as circular bacteriocins and leaderless bacteriocins are gaining more attention due to their enormous potential for industrial application. Circular bacteriocins and leaderless bacteriocins, arguably the least understood groups of bacteriocins, possess distinctively peculiar characteristics in their structures and biosynthetic mechanisms. Circular bacteriocins have N-to-C- terminal covalent linkage forming a structurally distinct circular peptide backbone. The circular nature of their structures provides them superior stability against various stresses compared to most linear bacteriocins. The molecular mechanism of their biosynthesis, albeit has remained poorly understood, is believed to possesses huge application prospect as it can serve as scaffold in bioengineering other biologically important peptides. On the other hand, while most bacteriocins are synthesized as inactive precursor peptides, which possess an N-terminal leader peptide attached to a C-terminal propeptide, leaderless bacteriocins are atypical as they do not have an N-terminal leader peptide, hence the name. Leaderless bacteriocins are active right after translation as they do not undergo any post-translational processing common to other groups of bacteriocins. This “simplicity” in the biosynthesis of leaderless bacteriocins offers a huge commercial potential as scale-up production systems are considerably easier to assemble. In this review, we summarize the current studies of both circular and leaderless bacteriocins, highlighting the progress in understanding their biosynthesis, mode of action, application and their prospects.

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Section: Applications Challenges and Prospectsmentioning

confidence: 99%

Circular and Leaderless Bacteriocins: Biosynthesis, Mode of Action, Applications, and Prospects

2018

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“…As a result, thucin A1 exhibited no hemolytic activity and cytotoxicity, even at concentrations much higher than its MIC values against a variety of indicator bacteria. In addition, recent studies have suggested that many leaderless bacteriocins, such as aureocin A53, aureocinA70 and toyoncin, can effectively inhibit or eliminate foodborne pathogens in skim milk, and possess desirable features to be used as food preservatives [ 1 , 42 , 50 ]. In the present study, we also observed that thucin A1 effectively inhibited or eliminated foodborne pathogens B. cereus and L. monocytogenes in skim milk.…”

Section: Discussionmentioning

confidence: 99%

“…Food pathogenic or spoilage bacteria pose serious threats to food quality and human health [ 1 – 3 ]. Chemical preservatives generally have certain detrimental effects, and therefore consumers show increasing demand for safer, healthier and natural biopreservatives [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Three novel leaderless bacteriocins have antimicrobial activity against gram-positive bacteria to serve as promising food biopreservative

et al. 2022

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Background Due to the detrimental effects of chemical preservatives, there has been an increasing demand for safer, healthier and natural bio-preservatives. Bacteriocins have attracted increasing interest because of their potential as natural bio-preservatives. Results We screened a large number of Bacillus thuringiensis strains and isolated one strain (B. thuringiensis P86) with antimicrobial activity against several foodborne pathogens. Three novel leaderless bacteriocins, including thucin A1, thucin A2 and thucin A3, were purified and identified from the culture supernatant of B. thuringiensis P86, whose molecular masses were 5552.02, 5578.07 and 5609.06 Da, respectively. Thucin A1 was then selected as a representative to be tested, and it exhibited potent inhibitory activity against all tested gram-positive bacteria. More importantly, thucin A1 showed stronger antimicrobial activity than nisin A against two important foodborne pathogens Bacillus cereus and Listeria monocytogenes. In addition, thucin A1 exhibited strong acid–base adaptability (pH 2–11), high endurance to heat, good stability to trypsin and pepsin, no hemolysis activity and cytotoxicity, and could effectively inhibit or eliminate Bacillus cereus and Listeria monocytogenes in skim milk. Conclusions Our findings indicate that these novel leaderless bacteriocins are potentially promising food biopreservatives.

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“…Despite this, our results serve as a proof of principle. There are different types of LLBs, such as aureocin A53 ( 41 , 42 ) and weissellicin YM ( 43 ), and mature peptides from other nonmodified bacteriocins can also be the candidates for LLB-phage design. In addition to many choices of LLBs, there are an almost countless number of phages, meaning that in the future, it may be possible to produce any number of LLB-phages which can be designed to efficiently control different types of bacterial contamination in different settings.…”

Section: Discussionmentioning

confidence: 99%

Construction of Leaderless-Bacteriocin-Producing Bacteriophage Targeting E. coli and Neighboring Gram-Positive Pathogens

et al. 2021

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The antimicrobial peptide aureocin A53 as an alternative agent for biopreservation of dairy products

Abstract: Taken together, the results confirmed that aureocin A53 has potential to be used as a food preservative, representing an alternative to the use of nisin in biopreservation of dairy products.

Cited by 21 publications

References 44 publications

Circular and Leaderless Bacteriocins: Biosynthesis, Mode of Action, Applications, and Prospects

Circular and Leaderless Bacteriocins: Biosynthesis, Mode of Action, Applications, and Prospects

Three novel leaderless bacteriocins have antimicrobial activity against gram-positive bacteria to serve as promising food biopreservative

Construction of Leaderless-Bacteriocin-Producing Bacteriophage Targeting E. coli and Neighboring Gram-Positive Pathogens

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