The Cellular Bioeffects of Low Intensity Ultrasound

Martin, Eleanor

doi:10.1179/174227109x12500735818025

Cited by 7 publications

(6 citation statements)

References 58 publications

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“…Although the therapeutic potential of LIPUS and sonoporation is well recognized, the biological grounding in our current scientific understanding of these two phenomena has remained to be critically weak. In the case of LIPUS, its stimulatory benefits are sometimes controversial [10]- [12], and a stronger knowledge of the underlying biological mechanisms is necessary to corroborate the surmised therapeutic value [13], [14]. In the case of sonoporation, its practical efficiency is deemed to be mediocre [15], [16], and the pursuit of detailed knowledge on the pertinent cellular bioeffects is seemingly essential to devise strategies for improving the overall efficiency of this membrane perforation approach [17], [18].…”

mentioning

confidence: 99%

Cellular Bioeffect Investigations on Low-Intensity Pulsed Ultrasound and Sonoporation: Platform Design and Flow Cytometry Protocol

Duan

Wan

2019

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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At low-intensity levels, ultrasound can potentially generate therapeutic effects on living cells, and it can trigger sonoporation when microbubbles (MBs) are present to facilitate drug delivery. Yet, our foundational knowledge of low-intensity pulsed ultrasound (LIPUS) and sonoporation remains to be critically weak because the pertinent cellular bioeffects have not been rigorously studied. In this article, we present a populationbased experimental protocol that can effectively foster investigations on the mechanistic bioeffects of LIPUS and sonoporation over a cell population. Walkthroughs of different methodological details are presented, including the fabrication of the ultrasound exposure platform and its calibration, as well as the design of a bioassay procedure that uses fluorescent tracers and flow cytometry to isolate sonicated cells with similar characteristics. An application example is also presented to illustrate how our protocol can be used to investigate the downstream cellular bioeffects of leukemia cells. We show that, with 1-MHz LIPUS exposure (with 29.1 J/cm 2 delivered acoustic energy density), variations in viability and morphology would be found among different types of sonicated leukemia cells (HL-60, Molt-4) in the absence and presence of MBs. Taken altogether, this article provides a reference on how cellular bioeffect experiments on LIPUS and sonoporation can be planned meticulously to acquire strong observations that are critical to establish the biological foundations for therapeutic applications.

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mentioning

confidence: 99%

Cellular Bioeffect Investigations on Low-Intensity Pulsed Ultrasound and Sonoporation: Platform Design and Flow Cytometry Protocol

Duan

Wan

2019

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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“…Low-intensity pulsed ultrasound (LIPUS), as a physical stimulus, has the advantages of being non-invasive, portable, and targeted. Research shows that LIPUS can promote cell proliferation and differentiation in fibroblasts, Schwann cells, and other cell types [ 13 ], inhibit bone loss in ovariectomized rats or induced by weightlessness [ 14 , 15 ], and accelerate the fracture repair process [ 15 , 16 ]. Muscle atrophy is often accompanied by disorders of protein metabolism in muscle tissue, the increased decomposition of muscle protein, and the thinning or even disappearance of muscle fibers [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Integrated Application of Low-Intensity Pulsed Ultrasound in Diagnosis and Treatment of Atrophied Skeletal Muscle Induced in Tail-Suspended Rats

Yang

Lei

et al. 2022

IJMS

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Long-term exposure to microgravity leads to muscle atrophy, which is primarily characterized by a loss of muscle mass and strength and reduces one′s functional capability. A weightlessness-induced muscle atrophy model was established using the tail suspension test to evaluate the intervention or therapeutic effect of low-intensity pulsed ultrasound (LIPUS) on muscle atrophy. The rats were divided into five groups at random: the model group (B), the normal control group (NC), the sham-ultrasound control group (SUC), the LIPUS of 50 mW/cm2 radiation group (50 UR), and the LIPUS of 150 mW/cm2 radiation group (150 UR). Body weight, gastrocnemius weight, muscle force, and B-ultrasound images were used to evaluate muscle atrophy status. Results showed that the body weight, gastrocnemius weight, and image entropy of the tail suspension group were significantly lower than those of the control group (p < 0.01), confirming the presence of muscle atrophy. Although the results show that the muscle force and two weights of the rats stimulated by LIPUS are still much smaller than those of the NC group, they are significantly different from those of the pure tail suspension B group (p < 0.01). On day 14, the gastrocnemius forces of the rats exposed to 50 mW/cm2 and 150 mW/cm2 LIPUS were 150% and 165% of those in the B group. The gastrocnemius weights were both 135% of those in the B group. This suggests that ultrasound can, to a certain extent, prevent muscular atrophy.

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“…LIPUS is used for stimulating healing in different tissues for a variety of applications, especially in rehabilitation medicine, bone-fracture healing, soft-tissue regeneration, and inflammatory responses inhibition. At a cellular level, LIPUS has also been observed to increase migration and proliferation of aortic endothelial cells, proliferation of fibroblasts, Schwann cells, and other cell types [5].…”

Section: Introductionmentioning

confidence: 99%

Biophysical and Biomechanical Effect of Low Intensity US Treatments on Pancreatic Adenocarcinoma 3D Cultures

et al. 2022

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Current developments in medical technology have focused on therapeutic treatments that selectively and effectively address specific pathological areas, minimizing side effects on healthy tissues. In this regard, many procedures have been developed to provide non-invasive therapy, for example therapeutic ultrasound (US). In the medical field, in particular in cancer research, it has been observed how ultrasounds can cause cell death and inhibit cell proliferation of cancer cells, while preserving healthy ones with almost negligible side effects. Various studies have shown that low intensity pulse ultrasound (LIPUS) and low intensity continuous ultrasound (LICUS) regulate the proliferation, cell differentiation and cavitation phenomena. Nowadays, there are poorly known aspects of low intensity US treatment, in terms of biophysical and biomechanical effects on target cells. The aim of this study is to set up an innovative apparatus for US treatment of pancreatic ductal adenocarcinoma (PDAC) cells, monitoring parameters such as acoustic intensity, acoustic pressure, stimulation frequency and treatment protocol. To this purpose, we have developed a custom-made set up for the US stimulation at 1.2 and 3 MHz of tridimensional (3D) cultures of PDAC cells (PANC-1, Mia Paca-2 and BxPc3 cells). Images of the 3D cultures were acquired, and the Calcein/PI assay was applied to detect US-induced cell death. Overall, the setup we have presented paves the way to an innovative protocol for tumor treatment. The system can be used either alone or in combination with small molecules or recombinant antibodies in order to propose a novel combined therapeutic approach.

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The Cellular Bioeffects of Low Intensity Ultrasound

Cited by 7 publications

References 58 publications

Cellular Bioeffect Investigations on Low-Intensity Pulsed Ultrasound and Sonoporation: Platform Design and Flow Cytometry Protocol

Cellular Bioeffect Investigations on Low-Intensity Pulsed Ultrasound and Sonoporation: Platform Design and Flow Cytometry Protocol

Integrated Application of Low-Intensity Pulsed Ultrasound in Diagnosis and Treatment of Atrophied Skeletal Muscle Induced in Tail-Suspended Rats

Biophysical and Biomechanical Effect of Low Intensity US Treatments on Pancreatic Adenocarcinoma 3D Cultures

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