The chromosomes of Nycticebus coucang (Boddaert, 1785) (Primates: Prosimii)

Stanyon, Roscoe; Masters, Judith; Romagno, Daniela

doi:10.1007/bf00055258

Cited by 11 publications

(20 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 ). Since the DAPI-band pattern is similar to those of G-bands, we were able to compare our results with G-banded karyotypes previously described for the closely related species N. coucang [Stanyon et al, 1987;Nie et al, 2006]. The karyotypes of these species were very similar to each other ( fig.…”

Section: Resultsmentioning

confidence: 50%

“…1 , 2 ). The results suggest that N. bengalensis experienced a higher genomic dispersion of rDNA in its evolutionary history than N. coucang , N. pygmaeus, or N. intermedius [Stanyon et al, 1987;Cheng et al, 1993;Warter and Rumpler, 1998]. Multiple manifestations of rDNA loci, in turn, suggest a mechanism of ectopic recombination through association of rDNA and other chromosomal segments during cell division ( fig.…”

Section: Discussionmentioning

confidence: 91%

“…A comparison with previous studies [Stanyon et al, 1987;Chen et al, 1993;Water and Rumpler, 1997;Nie et al, 2006] reveals similarities and differences between N. coucang and N. bengalensis . The most remarkable differences were the localizations of NORs.…”

Section: Discussionmentioning

confidence: 97%

“…Though the Indochinese forms were long classified as a single taxon (N. coucang) [Hill, 1952], 3 species have recently been identified (N. coucang , N. pygmaeus , and N. bengalensis) by variations in their skull characteristics [Groves, 1998[Groves, , 2001. Chromosome analyses of N. coucang , N. pygmaeus, and N. intermedius (included in N. pygmaeus ) [Brandon-Jones et al, 2004] have revealed a largely standard karyotype (2n = 50); however, the number of nucleolus organizer regions (NORs) were found to be variable through silver nitrate staining (Ag-NOR stain-ing), which revealed 6 or 10 loci among the 3 species [Stanyon et al, 1987;Chen et al, 1993;Warter and Rumpler, 1998]. However, the precise number and chromosomal localizations of the NORs remain unclear, because Ag-NORs techniques show wide variance in stainability.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Hypervariability of Nucleolus Organizer Regions in Bengal Slow Lorises, Nycticebus bengalensis (Primates, Lorisidae)

Baicharoen

Hirai²,

Srikulnath³

et al. 2016

Cytogenet Genome Res

View full text Add to dashboard Cite

Slow lorises are a cryptic species complex, and thus genetic markers are needed to identify distinct evolutionary lineages or species. We examined the nucleolus organizer regions (NORs) of Bengal slow lorises (Nycticebus bengalensis) using FISH with 18S rDNA (rDNA-FISH) and silver nitrate staining (Ag-NOR stain). Ten individuals of the putatively single species N. bengalensis showed higher variability in localization than 3 other congeners, though their overall karyotypes were similar. The rDNA-FISH analysis detected a total of 18 loci, in contrast to previous studies of other slow loris species that revealed far fewer (6-10) loci. Eight of the 18 loci detected in the present analysis were found to be semi-stable localizations at 4 different chromosomes, while 10 were found to be unstable localizations at 5 other chromosomes. The semi-stable locations showed occasional presence/absence of variations for rDNA-FISH, and unstable locations were polymorphic among individuals, contributing to the higher variability of NORs in this taxon. We hypothesize that the larger numbers of rDNA loci found in N. bengalensis were introduced by genomic dispersion through ectopic recombination in association with terminal regions including rDNA. Such differences are potentially very powerful chromosomal markers to be used in species identification and conservation.

show abstract

Section: Resultsmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 99%

See 2 more Smart Citations

Hypervariability of Nucleolus Organizer Regions in Bengal Slow Lorises, Nycticebus bengalensis (Primates, Lorisidae)

Baicharoen

Hirai²,

Srikulnath³

et al. 2016

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…Although lorisiform primates are distributed in both Asia and Africa, they are considered a monophyletic group and share a common ancestry based on fossil records, morphology, behavior, and molecular studies (Rasmussen and Nekaris, 1998). Previous conventional karyotypic analyses indicated that lorisiform primates probably evolved from a common ancestor with a diploid number of 2n ¼ 62, and that its karyotype was similar to that of the greater bushbaby (Otolemur crassicaudatus, OCR, 2n ¼ 62) (de Boer, 1973a-c;Dutrillaux, 1979;Dutrillaux et al, 1982;Rumpler et al, 1983Rumpler et al, , 1987Master et al, 1987;Stanyon et al, 1987). An earlier chromosomal phylogeny, based on comparative banding analysis, suggests that the 2n ¼ 50 karyotype of slow loris (Nycticebus coucang, NCO) evolved from the 2n ¼ 62 ancestral lorisiform karyotype via nine pericentric inversions, seven interchromosomal rearrangements, and the acquisition of heterochromatin on nine chromosomes (Rumpler et al, 1987).…”

mentioning

confidence: 96%

Chromosome painting between human and lorisiform prosimians: Evidence for the HSA 7/16 synteny in the primate ancestral karyotype

Nie

O’Brien

et al. 2005

American J Phys Anthropol

View full text Add to dashboard Cite

Multidirectional chromosome painting with probes derived from flow-sorted chromosomes of humans (Homo sapiens, HSA, 2n = 46) and galagos (Galago moholi, GMO, 2n = 38) allowed us to map evolutionarily conserved chromosomal segments among humans, galagos, and slow lorises (Nycticebus coucang, NCO, 2n = 50). In total, the 22 human autosomal painting probes detected 40 homologous chromosomal segments in the slow loris genome. The genome of the slow loris contains 16 sytenic associations of human homologues. The ancient syntenic associations of human chromosomes such as HSA 3/21, 7/16, 12/22 (twice), and 14/15, reported in most mammalian species, were also present in the slow loris genome. Six associations (HSA 1a/19a, 2a/12a, 6a/14b, 7a/12c, 9/15b, and 10a/19b) were shared by the slow loris and galago. Five associations (HSA 1b/6b, 4a/5a, 11b/15a, 12b/19b, and 15b/16b) were unique to the slow loris. In contrast, 30 homologous chromosome segments were identified in the slow loris genome when using galago chromosome painting probes. The data showed that the karyotypic differences between these two species were mainly due to Robertsonian translocations. Reverse painting, using galago painting probes onto human chromosomes, confirmed most of the chromosome homologies between humans and galagos established previously, and documented the HSA 7/16 association in galagos, which was not reported previously. The presence of the HSA 7/16 association in the slow loris and galago suggests that the 7/16 association is an ancestral synteny for primates. Based on our results and the published homology maps between humans and other primate species, we propose an ancestral karyotype (2n = 60) for lorisiform primates.

show abstract

Multidirectional chromosome painting reveals a remarkable syntenic homology between the greater galagos and the slow loris

Stanyon

Dumas

Stone

et al. 2006

American J Primatol

Self Cite

View full text Add to dashboard Cite

We report on the first reciprocal chromosome painting of lorisoids and humans. The chromosome painting showed a remarkable syntenic homology between Otolemur and Nycticebus. Eight derived syntenic associations of human segments are common to both Otolemur and Nycticebus, indicative of a considerable period of common evolution between the greater galago and the slow loris. Five additional Robertsonian translocations form the slow loris karyotype, while the remaining chromosomes are syntenically equivalent, although some differ in terms of centromere position and heterochromatin additions. Strikingly, the breakpoints of the human chromosomes found fragmented in these two species are apparently identical. Only fissions of homologs to human chromosomes 1 and 15 provide significant evidence of a cytogenetic link between Lemuriformes and Lorisiformes. The association of human chromosomes 7/16 in both lorisoids strongly suggests that this chromosome was present in the ancestral primate genome.

show abstract

The chromosomes of Nycticebus coucang (Boddaert, 1785) (Primates: Prosimii)

Cited by 11 publications

References 20 publications

Hypervariability of Nucleolus Organizer Regions in Bengal Slow Lorises, <b><i>Nycticebus bengalensis</i></b> (Primates, Lorisidae)

Hypervariability of Nucleolus Organizer Regions in Bengal Slow Lorises, <b><i>Nycticebus bengalensis</i></b> (Primates, Lorisidae)

Chromosome painting between human and lorisiform prosimians: Evidence for the HSA 7/16 synteny in the primate ancestral karyotype

Multidirectional chromosome painting reveals a remarkable syntenic homology between the greater galagos and the slow loris

Contact Info

Product

Resources

About