The Comparison of Doxycycline Residue in the Meat of Broiler Chickens Administered in Feed and Water

Wijayanti, Agustina Dwi; Wihandoyo, .; Rosetyadewi, Antasiswa Windraningtyas

doi:10.5398/medpet.2011.34.3.175

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…The value of feed intake obtained in this experiment was directly proportional to body weight gain for each treatment, so the feed conversion rate was also not different. According to Wijayanti (2011), the high and low feed conversion rate is caused by the feed consumed and the body weight gain achieved. Thus, the high or low feed conversion rate is influenced by the feed consumption and the body weight gain obtained.…”

Section: Discussionmentioning

confidence: 99%

Impact of Utilization of Brown Seaweed Sargassum crassifolium in Broilers Diet as a Substitution of Traditional Feed Ingredients

Endo Mahata,

Rizal,

Zurmiati

et al. 2023

JAHP

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Due to increasing trend in prices of traditional feed ingredients, researchers are exploring marine resources as a novel cheap substitutions for poultry industry. This study aims to determine the effect of partial replacement of traditional feed ingredients like corn and rice bran with brown seaweed Sargassum crassifolium on broiler performance. The study employed a fully randomized design, incorporating four replacement treatments (0%, 6%, 12%, and 18%) of brown seaweed Sargassum crassifolium within the broiler diet. Each treatment was replicated five times. A total of 100-day-old chicks were used in the experiment and reared under standard conditions. The results showed that inclusion of brown seaweed Sargassum crassifolium up to 18% in broiler diet did not affect (p>0.05) daily feed intake, daily weight gain, feed conversion ratio, live weight, carcass percentage with skin and without skin; however, it significantly (p<0.05) reduced the abdominal fat pad percentage of broiler. In conclusion, the inclusion of brown seaweed Sargassum crassifolium in the broiler diet up to 18% reduces corn utilization by 24.49% and rice bran by 54.45% at the starter period and reduces corn up to 21% and rice bran by 50% at the grower period without interfering with their performance.

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Section: Discussionmentioning

confidence: 99%

Impact of Utilization of Brown Seaweed Sargassum crassifolium in Broilers Diet as a Substitution of Traditional Feed Ingredients

Endo Mahata,

Rizal,

Zurmiati

et al. 2023

JAHP

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“…Для вивчення брали чисту плазму курей (попередньо перевірену методом УЕРХ-МС/МС на вміст доксицикліну), збагачували стандартним розчином доксицикліну на рівні 1 і 5 мкг/мл, і послідовно розводили в 5000 разів буфером для розведення зразків, що надається у наборі, для того, щоб отримані розчини, які за вмістом аналіту потрапляли в концентраційний інтервал калібрувальної кривої, і далі проводили аналіз імуноферментним методом згідно з інструкцією. Для навантаження обрали такі концентрації доксицикліну, порядок яких близький до вмісту його в плазмі крові курей-бройлерів при лікуванні їх препаратами на основі доксицикліну (Anadon et al, 1994;Laczay et al, 2001;Wijayanti et al, 2011;Hsiao et al, 2016;Pai-Feng at al., 2016;Yang et al, 2018). Для перевірки правильності та ефективності такого підходу, ці ж зразки було проаналізовано методом УЕРХ-МС/МС, використовуючи калібрувальний графік в інтервалі 0 -10,0 мкг/мл, побудований на матриці чистого зразка плазми крові курчат-бройлерів.…”

Section: рис 1 хімічна структура доксициклінуunclassified

Doxycycline Determination in Animal Tissues and Blood Plasma Samples Using Screening and Confirmatory Methods

Yanovych

Zasadna²,

Rydchuk

et al. 2021

SCIVP

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The article presents the results of validation of sample preparation method for muscle and parenchymal tissues of animals for further determination of doxycycline residual amounts by enzyme-linked immunosorbent assay using Oxytetracycline ELISA test kits from EuroProxima (Netherlands), initially developed for the analysis of other target matrices, viz. honey and shrimp. The results of modification of shrimp sample preparation method for the analysis of muscle and parenchymal tissue samples are showed. In accordance with the European Commission Decision 2002/657/EC, the proposed method was validated taking into account the maximum residue levels (MRLs) of doxycycline in tissues of productive animals: 100 μg/kg for muscle tissue and 300 μg/kg for liver. The suitability of the technique was confirmed on the basis of the established main validation parameters for screening methods (technical threshold and cut-off factor) using control (pure) and spiked with standard doxycycline solution at the level of ½MRL samples of muscle and parenchymal tissues of broiler chickens by means of “added – found” criterion. It was discovered that this approach to sample preparation makes it possible to determine doxycycline residues in poultry tissues at the level of 5-10 μg/kg with the absolute recovery about 54 %. The main advantages of the developed technique are simplicity of performance, rapidity and cost-effectiveness. The reliability of the results obtained by the screening method was verified using confirmatory UPLC-MS/MS method, viz. a comparative study of blank and spiked samples of muscle and parenchymal tissues of broiler chickens. The method of sample preparation of the studied specimens, the conditions of analysis carrying out, and the main validation parameters of the confirmatory method are also presented. Based on the obtained results, it was concluded that the developed screening method of enzyme-linked immunosorbent assay is suitable for the determination of doxycycline residual amounts in muscle and parenchymal tissues samples, and it is possible to use it for routine laboratory tests, as well as for the establishment of withdrawal periods of veterinary drugs based on doxycycline from the tissues of productive animals. Also using the same test kit, the sample preparation method for blood plasma samples for doxycycline content assay (at the levels of μg/ml) was suggested to study the bioequivalence of veterinary drugs containing doxycycline as an active pharmaceutical ingredient, which was as well confirmed by UPLC-MS/MS.

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“…Though research related with antibiotic residue is limited in Bangladesh but many countries have detected residue in food chain. (Hussain et al, 2013;Wijayanti et al, 2012;Zhao et al, 2009;Pent et al, 2010;Sattar et al, 2014). This study assures the presence of oxytetracycline and doxycycline residue in broiler meat.…”

Section: Assessment Of Antibiotic Residuementioning

confidence: 99%

Detection of oxytetracycline and doxycycline residue in different growth stages of commercial broiler

2019

BJVM

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Background: Indiscriminant use of antibiotic in broiler farms is responsible for residual deposition in broiler’s edible tissue that can possess health hazard including antibiotic resistance to human being by entering in food chain. This study was designed to detect and compare two common antibiotics residue in broiler at three different growth stages. Methods: A questionnaire survey was conducted to understand the farmers’ perspective regarding antibiotic residue in four upazila of Mymensingh district. Samples were collected from eight poultry farms of each upazila namely Fulbaria, Trishal, Nakla and Mymensingh Sadar. From each farm, nine samples were collected at the three different ages of 9-17 days, 19-22 days and 29-32 days, respectively. Thin layer chromatography (TLC) was used for the qualitative detection of oxytetracycline and doxycycline residues in broiler meat. Data was analyzed by SPSS IBM 20. Results: Most of the farmers are educated up to secondary level. Only 9.37% farmers have residual knowledge but all are unaware about withdrawal period. No one is practicing good management system for their flock. A total of 288 meat samples were analyzed from four upazila. Oxytetracycline residue was detected in >50% samples: in Fulbaria (55.56%), Trisal (66.66%) and in Nakla (55.55%) upazila and slightly lower in Mymensingh Sadar (44.44%). Similar observation was obtained for doxycycline: Fulbaria (44.44%), Mymensingh Sadar (66.67%), in Nakla (55.55%) and in Trishal (44.44%). The residual level detected positive in first, second and third collections were 66.67%, 25% and 58.33% for oxytetracycline and doxycycline, respectively. Conclusions: These result indicates that doxycycline and oxytetracycline are being misused in poultry industry especially in first and last quarter of broiler life and withdrawal periods are not maintained when broiler are marketed. Measures are needed to ensure human safety regarding antibiotics use in poultry industry.

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The Comparison of Doxycycline Residue in the Meat of Broiler Chickens Administered in Feed and Water

Cited by 3 publications

References 11 publications

Impact of Utilization of Brown Seaweed Sargassum crassifolium in Broilers Diet as a Substitution of Traditional Feed Ingredients

Impact of Utilization of Brown Seaweed Sargassum crassifolium in Broilers Diet as a Substitution of Traditional Feed Ingredients

Doxycycline Determination in Animal Tissues and Blood Plasma Samples Using Screening and Confirmatory Methods

Detection of oxytetracycline and doxycycline residue in different growth stages of commercial broiler

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