The complete mitochondrial genome of the emperor dragonflyAnax imperatorLEACH, 1815 (Odonata : Aeshnidae) via NGS sequencing

Abstract

Here we report the complete mitochondrial genome of the emperor dragonfly, Anax imperator (Odonata: Aeshnidae) as the first of its genus. Data were generated via next generation sequencing (NGS) and assembled using an iterative approach. The typical metazoan set of 37 genes (13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes) was detected in the same gene order as in other odonate mitogenomes. However, only three intergenic spacer regions are present in A. imperator lacking the distinct s5 spacer, which was regarded as informative feature of the odonate suborder Anisoptera (dragonflies) but absent in Zygoptera (damselflies). With 16,087 bp, it is the longest anisopteran mitogenome to date, mainly due to the long A + T-rich control region of 1291 bp.

The complete mitochondrial genome of the neotropical helicopter damselflyMegaloprepus caerulatus(Odonata: Zygoptera) assembled from next generation sequencing data

“…In all observed parameters, the presented mitochondrial genome shows a strong similarity to all other already published odonate mitochondrial genomes (e.g. Lorenzo-Carballa et al 2014;Tang et al 2014;Yu et al 2014;Chen et al 2015;Feindt et al 2016;Herzog et al 2016). It contains the common arrangement of 37 genes including 13 protein-coding genes, 2 rRNA (16S and 12S rRNA) genes, 22 tRNA genes, and an A þ T rich (control) region of 1376 bp in length.…”

The mitochondrial genome of Atrocalopteryx melli Ris, 1912 (Zygoptera: Calopterygidae) via Ion Torrent PGM NGS sequencing

“…Moreover, there are three intergenic spacers with sequence lengths from 9 bp to 16 bp. The S5 spacer is absent in A. melli, supporting the earlier finding of a lack of S5 spacer in damselflies (Lin et al 2010), although this finding could not be universal for that group (Herzog et al 2016). Atrocalopteryx melli and A. atrata (NC_027181.1) have the same mitochondrial genes order and composition.…”

“…tRNA genes have lengths from 64 bp to 72 bp, and they can be folded in CONTACT Qiuqi Lin tlinqq@jnu.edu.cn Institute of Hydrobiology, Jinan University, Guangzhou, China the typical cloverleaf structure. The control region (A þ T rich: 79%) has a length of 928 bp, shorter than most other Odonata (Lee et al 2009;Herzog et al 2016). Moreover, there are three intergenic spacers with sequence lengths from 9 bp to 16 bp.…”

Complete mitochondrial genome sequence of Bekko Tombo Libellula angelina Selys, 1883 (Odonata: Libellulidae)

“…Two species belonging to the order Ephemeroptera were used as outgroups. GenBank accession numbers are as follows: Euphaea ornata, KF718295 (Cheng et al 2018); Euphae decorata, KF718294 (Cheng et al 2018); Euphae formosa, HM126547 (Lin et al 2010); Euphae yayeyamana, KF718293 (Cheng et al 2018); Pseudolestes mirabilis, FJ606784 (unpublished); Mnais costalis, KU871065 (Lorenzo-Carballa et al 2016); Vestalis melania, JX050224 (Chen et al 2015); Atrocalopteryx atrata, KP233805 (unpublished); Ischnura elegans, KU958378 (Feindt et al 2016a); Ischnura pumilio, KC878732 (Lorenzo-Carballa et al 2014); Megaloprepus caerulatus, KU958377 (Feindt et al 2016b); Platycnemis foliacea, KP233804 (unpublished); Epiophlebia superstes, JX050223 (Wang et al 2015); Orthetrum chrysis, KU361233 (Yong et al 2016); Orthetrum testaceum, KU361235 (Yong et al 2016); Orthetrum glaucum, KU361232 (Yong et al 2016); Orthetrum sabina, KU361234 (Yong et al 2016); Nannophya pygmaea, KY402222 (Jeong et al 2018); Hydrobasileus croceus, KM244659 (Tang et al 2014); Brachythemis contaminata, KM658172 (Yu et al 2016); Macromia daimoji MF990748 (Kim et al 2018); Anax imperator, KX161841 (Herzog et al 2016); Davidius lunatus, EU591677 (Lee et al 2009); Ictinogomphus sp., KM244673 (Tang et al 2014); Isonychia ignota, HM143892 (unpublished); and Ephemera orientalis, EU591678 (Lee et al 2009).…”